Results 21 to 30 of about 12,345 (165)

PARP1 and PARP2 are dispensable for DNA repair by microhomology-mediated end-joining at double-ended DSBs. [PDF]

Nucleic Acids Res
Poly ADP-ribose polymerase (PARP) inhibitors are standard of care treatment for cancers with homologous-recombination deficiencies. Yet, as tumours develop resistance, complementary strategies are emerging, including targeting microhomology-mediated end ...
Ortega R, Taylor ER, Whitehead SM, Danhorn T, Bitler BG, Arnoult N.   +5 more
europepmc   +4 more sources

Osteogenesis Imperfecta with a gross deletion including the COL1A1 gene, induced by Alu-driven microhomology-mediated end joining [PDF]

Bone Reports
Osteogenesis Imperfecta (OI) is a rare hereditary brittle bone disorder typically caused by COL1A1 and COL1A2 variants impairing type I collagen. However, gross deletions involving COL1A1 are uncommon.
Kenichi Yamamoto, Hirofumi Nakayama, Yusaku Ito, Masaya Hattori, Takaaki Shimada, Ikumi Ueda, Takeshi Ishimi, Chieko Yamada, Yukako Nakano, Makoto Fujiwara, Takuo Kubota, Yasuhisa Ohata, Yasuji Kitabatake   +12 more
doaj   +3 more sources

Chromosomal Translocations in the Parasite Leishmania by a MRE11/RAD50-Independent Microhomology-Mediated End Joining Mechanism [PDF]

PLoS Genetics, 2016
The parasite Leishmania often relies on gene rearrangements to survive stressful environments. However, safeguarding a minimum level of genome integrity is important for cell survival.
Marie-Claude N Laffitte, Philippe Leprohon, Danielle Legare   +2 more
exaly   +7 more sources

Microhomology-mediated end-joining Knock-In approaches to delete the allergenic domain of trout parvalbumin beta-1. Preliminary results in F0 animals and feedback [version 2; peer review: 1 approved, 2 approved with reservations] [PDF]

Open Research Europe
Background Gene editing techniques offer new opportunities to improve important traits in aquaculture. The allergenicity of fish flesh is a major problem in aquaculture. Parvalbumin (Parv) is the most prevalent fish allergen.
Amaury Herpin, Cecile Duret, Pierre-yves Rescan, Veronique Lebret   +3 more
doaj   +3 more sources

Microhomology Selection for Microhomology Mediated End Joining in Saccharomyces cerevisiae. [PDF]

Genes (Basel), 2019
Microhomology-mediated end joining (MMEJ) anneals short, imperfect microhomologies flanking DNA breaks, producing repair products with deletions in a Ku- and RAD52-independent fashion.
Lee K, Ji JH, Yoon K, Che J, Seol JH, Lee SE, Shim EY.   +6 more
europepmc   +5 more sources

Microhomology-mediated end joining: Good, bad and ugly [PDF]

Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 2018
DNA double-strand breaks (DSBs) are induced by a variety of genotoxic agents, including ionizing radiation and chemotherapy drugs for treating cancers. The elimination of DSBs proceeds via distinctive error-free and error-prone pathways. Repair by homologous recombination (HR) is largely error-free and mediated by RAD51/BRCA2 gene products.
Eun Yong Shim, Sang Eun Lee
exaly   +5 more sources

Risky business: Microhomology-mediated end joining [PDF]

Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 2016
Prevalence of microhomology (MH) at the breakpoint junctions in somatic and germ-line chromosomal rearrangements and in the programmed immune receptor rearrangements from cells deficient in classical end joining reveals an enigmatic process called MH-mediated end joining (MMEJ).
Supriya Sinha, Eun Yong Shim, Sang Eun Lee   +2 more
exaly   +5 more sources

Mechanism of microhomology-mediated end-joining promoted by human DNA polymerase θ [PDF]

Nature Structural and Molecular Biology, 2015
Microhomology-mediated end-joining (MMEJ) is an error-prone alternative double-strand break-repair pathway that uses sequence microhomology to recombine broken DNA. Although MMEJ has been implicated in cancer development, the mechanism of this pathway is unknown. We demonstrate that purified human DNA polymerase θ (Polθ) performs MMEJ of DNA containing
Tatiana Kent, Gurushankar Chandramouly, Shane McDevitt   +2 more
exaly   +5 more sources

Novel allogeneic CAR T-cell platform involving microhomology-mediated end joining repair and low off-targeting potential. [PDF]

Mol Ther Nucleic Acids
Several allogeneic chimeric antigen receptor (CAR) T-cell therapies in clinical trials rely on CRISPR-Cas genome editing, but the enzyme’s random repair mechanism increases the risk of undesired off-target effects, challenging safe CAR T-cell generation.
Hundal T, Luo Y, Qie Y, Gadd ME, Brim AD, Vazquez-Rosario I, Guo S, Kharfan-Dabaja MA, Qin H.   +8 more
europepmc   +2 more sources

Modulation of the microhomology-mediated end joining pathway suppresses large deletions and enhances homology-directed repair following CRISPR-Cas9-induced DNA breaks. [PDF]

BMC Biol, 2022
CRISPR-Cas9, an efficient genome editing tool, has been widely used in research and holds great promise in the clinic. However, large unintended rearrangements of the genome occur frequently after CRISPR-Cas9 editing and their potential risk cannot be ...
Yuan B, Bi C, Tian Y, Wang J, Jin Y, Alsayegh K, Tehseen M, Yi G, Zhou X, Shao Y, Romero FV, Fischle W, Izpisua Belmonte JC, Hamdan S, Huang Y, Li M.   +15 more
europepmc   +2 more sources