Results 41 to 50 of about 12,345 (165)
Drosophila DNA polymerase theta utilizes both helicase-like and polymerase domains during microhomology-mediated end joining and interstrand crosslink repair [PDF]
PLoS Genetics, 2017 Double strand breaks (DSBs) and interstrand crosslinks (ICLs) are toxic DNA lesions that can be repaired through multiple pathways, some of which involve shared proteins.
Robin L Armstrong +2 more
exaly +4 more sources
Beyond Short Microhomologies: Mismatch-Compatible Pol θ-Mediated DNA Damage Repair. [PDF]
BioessaysPol θ is traditionally thought to use a short, perfectly matched microhomology (MH) to initiate end‐joining repair of a DNA double‐strand break. Emerging evidence indicates that Pol θ generally utilizes mismatch‐compatible longer microhomologies. This revised definition of MH is applicable across species and broadens the definition of Pol θ‐associated ...
Li Y, Wood RD.
europepmc +2 more sources
Mitotic microhomology-mediated break-induced replication promotes chromoanasynthesis [PDF]
Nature CommunicationsChromoanasynthesis is a form of complex chromosomal rearrangement (CCR) commonly detected in cancers and congenital disorders, but the mechanism underlying its generation remain elusive. Here we develop a single-molecule long-read DNA sequencing approach
Greg H. P. Ngo +10 more
doaj +2 more sources
PLK1 targets CtIP to promote microhomology-mediated end joining. [PDF]
Nucleic Acids Res, 2018 Proper DNA double-strand break (DSB) repair is essential for maintaining genome integrity. Microhomology-mediated end joining (MMEJ) is an error-prone repair mechanism, which introduces mutations at break sites and contributes to chromosomal translocations and telomere fusions, thus driving carcinogenesis.
Wang H +11 more
europepmc +3 more sources
CRISPR/Cas-based precision genome editing via microhomology-mediated end joining. [PDF]
Plant Biotechnol J, 2021 SummaryGene editing and/or allele introgression with absolute precision and control appear to be the ultimate goals of genetic engineering. Precision genome editing in plants has been developed through various approaches, including oligonucleotide‐directed mutagenesis (ODM), base editing, prime editing and especially homologous recombination (HR)‐based
Van Vu T +7 more
europepmc +4 more sources
Microhomology-mediated end joining: new players join the team. [PDF]
Cell Biosci, 2017 DNA double-strand breaks (DSBs) are the most deleterious type of DNA damage in cells arising from endogenous and exogenous attacks on the genomic DNA. Timely and properly repair of DSBs is important for genomic integrity and survival. MMEJ is an error-prone repair mechanism for DSBs, which relies on exposed microhomologous sequence flanking broken ...
Wang H, Xu X.
europepmc +4 more sources
Molecular Therapy: Nucleic Acids, 2022 Gene editing utilizing homology-directed repair has advanced significantly for many monogenic diseases of the hematopoietic system in recent years but has also been hindered by decreases between in vitro and in vivo gene integration rates.
David H. Gray +7 more
doaj +1 more source
Frontiers in Genetics, 2022 DNA double-strand breaks (DSBs) can be repaired through various pathways. Understanding how these pathways are regulated is of great interest for cancer research and optimization of gene editing.
Ruben Schep +5 more
doaj +1 more source
, 2023 Source data for "The APE2 nuclease is essential for DNA double strand break repair by microhomology-mediated end-joining." Fleury et al ...
Arnoult, N
core +2 more sources
Helicase Q promotes homology-driven DNA double-strand break repair and prevents tandem duplications
Nature Communications, 2021 Microhomology-mediated end-joining (MMEJ) is a poorly defined mutagenic DNA break repair pathway. Here the authors show that the helicase HELQ is essential for polymerase theta-independent MMEJ, single-strand annealing and homologous recombination ...
J. A. Kamp +5 more
doaj +1 more source