Rad52 mediates class-switch DNA recombination to IgD
Nature Communications, 2022 IgD is expressed, predominantly together with IgM, via mRNA alternative splicing, but IgD class switch recombination (IgD CSR) has also been reported. Here the authors show, using Rad52-deficient mouse and human B cells, that IgD CSR is mediated by Rad52
Yijiang Xu +4 more
doaj +1 more source
Complex Breakpoints and Template Switching Associated with Non-canonical Termination of Homologous Recombination in Mammalian Cells. [PDF]
PLoS Genetics, 2016 A proportion of homologous recombination (HR) events in mammalian cells resolve by "long tract" gene conversion, reflecting copying of several kilobases from the donor sister chromatid prior to termination.
Andrea J Hartlerode +4 more
doaj +1 more source
Efficient CRISPR/Cas9-based plant genomic fragment deletions by microhomology-mediated end joining. [PDF]
Plant Biotechnol J, 2020 Tan J +14 more
europepmc +4 more sources
53BP1 promotes microhomology-mediated end-joining in G1-phase cells. [PDF]
Nucleic Acids Res, 2015 Abstract Alternative non-homologous end joining (alt-NHEJ) was originally identified as a backup repair mechanism in the absence of classical NHEJ (c-NHEJ) factors but recent studies have demonstrated that alt-NHEJ is active even when c-NHEJ as well as homologous recombination is available.
Xiong X +7 more
europepmc +4 more sources
Microhomology-mediated mechanisms underlie non-recurrent disease-causing microdeletions of the FOXL2 gene or its regulatory domain. [PDF]
PLoS Genetics, 2013 Genomic disorders are often caused by recurrent copy number variations (CNVs), with nonallelic homologous recombination (NAHR) as the underlying mechanism.
Hannah Verdin +10 more
doaj +1 more source
Biased genome editing using the local accumulation of DSB repair molecules system
Nature Communications, 2018 Genome editing using CRISPR can be enhanced by manipulating DNA double-strand break repair pathways. Here the authors demonstrate LoAD, local accumulation of repair molecules, which shifts repair to microhomology-mediated end-joining.
Shota Nakade +8 more
doaj +1 more source
Nature Communications, 2019 DNA repair by microhomology-mediated end joining creates precise deletions based on flanking microhomologies. Here the authors use CRISPR-Cas9 to recreate pathogenic deletion mutations using existing microhomologies in the human genome identified by ...
Janin Grajcarek +10 more
doaj +1 more source
Essential factors for incompatible DNA end joining at chromosomal DNA double strand breaks in vivo. [PDF]
PLoS ONE, 2011 Non-homologous end joining (NHEJ) is a major pathway for the repair of DNA double strand break (DSBs) with incompatible DNA ends, which are often generated by ionizing irradiation.
Hideaki Ogiwara, Takashi Kohno
doaj +1 more source
BRCA1 Facilitates Microhomology-mediated End Joining of DNA Double Strand Breaks [PDF]
Journal of Biological Chemistry, 2002 BRCA1 is critical for the maintenance of genomic stability, in part through its interaction with the Rad50.Mre11.Nbs1 complex, which occupies a central role in DNA double strand break repair mediated by nonhomologous end joining (NHEJ) and homologous recombination. BRCA1 has been shown to be required for homology-directed recombination repair. However,
Qing, Zhong +3 more
openaire +2 more sources
Microhomology-assisted scarless genome editing in human iPSCs
Nature Communications, 2018 Positive selection for gene targeting is a common and reliable method to generate isogenic disease models in human pluripotent stem cells. Here, the authors present engineered selection markers which achieve scarless excision by CRISPR-Cas9 and ...
Shin-Il Kim +10 more
doaj +1 more source