Results 31 to 40 of about 12,345 (165)

A role for human homologous recombination factors in suppressing microhomology-mediated end joining. [PDF]

Nucleic Acids Res, 2016
DNA double-strand breaks (DSBs) are toxic lesions, which if improperly repaired can result in cell death or genomic instability. DSB repair is usually facilitated by the classical non-homologous end joining (C-NHEJ), or homologous recombination (HR ...
Ahrabi S, Sarkar S, Pfister SX, Pirovano G, Higgins GS, Porter AC, Humphrey TC.   +6 more
europepmc   +7 more sources

DNA large fragment deleting by compact, sequence-motif-free and specific TaqTth-hpRNA assisted with the microhomology-mediated end joining pathway. [PDF]

Nucleic Acids Res
A DNA editing tool TaqTth-hpRNA was developed in this study, composed of a compact recombinant TaqTth nuclease (832 aa) and a simple hairpin-RNA guiding probe (hpRNA).
Liu Y, Weng Z, Zhai Z, Xu Z, Liu X, Qiang H, Tian K, Rao B, Zhou G, Guo Y, Xu S.   +10 more
europepmc   +2 more sources

Disruption of Microhomology-mediated End-joining in Ewing Sarcoma

bioRxiv
Ewing sarcoma (EwS) is a group of bone and soft tissue cancers in children and young adults. Since EwS cells have pronounced sensitivity to radiation and chemotherapy-induced DNA damage, the role of the oncoprotein, EWS-FLI1, in DNA repair is likely ...
S. Asada, G. Zhu, J. Abeykoon, Yutaro Tanaka, Huy Nguyen, Y. Hirohashi, Divya R Iyer, Nicholas W. Ashton, S. Mukkavalli, Martha Velázquez, Lige Jiang, M. Busso, J. Thangaiah, S. Robinson, K. Parmar, E. V. Van Allen, R. Gillani, G. I. Shapiro, Alan D. D’Andrea   +18 more
semanticscholar   +3 more sources

In vivo and in vitro knockout system labelled using fluorescent protein via microhomology-mediated end joining [PDF]

Life Science Alliance, 2020
This study provides a new method that enables the labelling of knockout cells with fluorescent protein through microhomology-mediated end joining (MMEJ)–based knock-in. Gene knockout is important for understanding gene function and genetic disorders. The
Shota Katayama, Kota Sato, Toru Nakazawa
doaj   +2 more sources

Microhomology-mediated end joining drives complex rearrangements and overexpression of MYC and PVT1 in multiple myeloma. [PDF]

Haematologica, 2020
MYC is a widely acting transcription factor and its deregulation is a crucial event in many human cancers. MYC is important biologically and clinically in multiple myeloma, but the mechanisms underlying its dysregulation are poorly understood.
Mikulasova A, Ashby C, Tytarenko RG, Qu P, Rosenthal A, Dent JA, Ryan KR, Bauer MA, Wardell CP, Hoering A, Mavrommatis K, Trotter M, Deshpande S, Yaccoby S, Tian E, Keats J, Auclair D, Jackson GH, Davies FE, Thakurta A, Morgan GJ, Walker BA.   +21 more
europepmc   +6 more sources

Publisher Correction: Molecular basis of microhomology-mediated end-joining by purified full-length Polθ [PDF]

Nature Communications, 2020
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Samuel J. Black, Ahmet Y. Ozdemir, Ekaterina Kashkina, Tatiana Kent, Timur Rusanov, Dejan Ristic, Yeonoh Shin, Antonio Suma, Trung Hoang, Gurushankar Chandramouly, Labiba A. Siddique, Nikita Borisonnik, Katherine Sullivan-Reed, Joseph S. Mallon, Tomasz Skorski, Vincenzo Carnevale, Katsuhiko S. Murakami, Claire Wyman, Richard T. Pomerantz   +18 more
doaj   +2 more sources

Particulate hexavalent chromium inhibits global transcription of genes in DNA repair pathways, particularly targeting homologous recombination repair, base excision repair, mismatch repair and microhomology-mediated end-joining. [PDF]

J Hazard Mater
Hexavalent chromium [Cr(VI)] is a human lung carcinogen with widespread exposure. How Cr(VI) causes cancer is poorly understood, but chromosome instability plays a central role.
Meaza I, Cahill CR, Speer RM, Kouokam JC, Wise JP.   +4 more
europepmc   +2 more sources

The APE2 nuclease is essential for DNA double strand break repair by microhomology-mediated end-joining

bioRxiv, 2022
Microhomology-mediated end-joining (MMEJ) is an intrinsically mutagenic pathway of DNA double strand break repair essential for proliferation of homologous recombination (HR) deficient tumors.
Fleury H, MacEachern MK, Stiefel CM, Anand R, Sempeck C, Nebenfuehr B, Dodd B, Taylor E, Dansoko D, Ortega R, Leung JW, Boulton SJ, Arnoult N.   +12 more
europepmc   +2 more sources

Microhomology-mediated end joining is the predominant form of DNA repair in the mosquito Aedes aegypti with implications for gene editing, gene drive, and transgene removal. [PDF]

Nucleic Acids Res
Programmable site-specific nucleases have revolutionized the field of genetics, and in the field of mosquito vector control, gene editing by these tools has inspired a new wave of population control approaches that aim to prevent disease transmission ...
Romanowski JS, Myles KM, Adelman ZN.
europepmc   +2 more sources

ATM regulates Mre11-dependent DNA end-degradation and microhomology- mediated end joining [PDF]

Cell Cycle, 2010
The human disorder ataxia telangiectasia (AT), which is characterized by genetic instability and neurodegeneration, results from mutation of the ataxia telangiectasia mutated (ATM) kinase.
Dixon K., Henricksen L.A., Tainer J.A., Li Y., Rahal E.A., Williams R.S.   +5 more
core   +3 more sources