Results 121 to 130 of about 4,873,130 (388)

A crude method of DNA extraction and identification from exfoliated human buccal mucosa cells

Indian Journal of Dental Research, 2019
Background: DNA analysis has a key role in forensic dentistry. However, techniques of DNA extraction and analysis are far from the reach of majority of medical professionals owing to its expensive set up. Aim: The present study was aimed at formulating a
Priyanka Kardam, Monica Mehendiratta, Shweta Rehani, Rashi Sharma, Khushboo Sahay   +4 more
doaj   +1 more source

Lipopolysaccharide is a frequent contaminant of plasmid DNA preparations and can be toxic to primary cells in the presence of adenovirus [PDF]

, 1994
Endotoxin (lipopolysaccharide, LPS) is commonly found as a contaminant in plasmid DNA preparations. We demonstrate here that the quantities of LPS typically contaminating DNA preparations can generate a toxicity to primary cells (primary human skin ...
Baker, A., Buschle, M., Cotten, Matt, Saltik, Mediyha, Wagner, Ernst   +4 more
core  

Impacts of DNA extraction and PCR on DNA metabarcoding estimates of soil biodiversity

Methods in Ecology and Evolution, 2018
Soils are ubiquitous and important components of terrestrial ecosystems, richly populated with diverse organisms with important ecological roles. DNA metabarcoding is a promising tool for efficient and taxonomically comprehensive analyses of soil ...
A. Dopheide, Dong Xie, T. Buckley, A. Drummond, R. Newcomb   +4 more
semanticscholar   +1 more source

In situ molecular organization and heterogeneity of the Legionella Dot/Icm T4SS

FEBS Letters, EarlyView.
We present a nearly complete in situ model of the Legionella Dot/Icm type IV secretion system, revealing its central secretion channel and identifying new components. Using cryo‐electron tomography with AI‐based modeling, our work highlights the structure, variability, and mechanism of this complex nanomachine, advancing understanding of bacterial ...
Przemysław Dutka, Yuxi Liu, Stefano Maggi, Debnath Ghosal, Jue Wang, Prashant P. Damke, Stephen D. Carter, Wei Zhao, Sukhithasri Vijayrajratnam, Joseph P. Vogel, Carrie L. Shaffer, Grant J. Jensen   +11 more
wiley   +1 more source

Ancient mitochondrial genomes recovered from small vertebrate bones through minimally destructive DNA extraction: phylogeography of the New Zealand gecko genus Hoplodactylus. [PDF]

, 2021
Lachie Scarsbrook, Alexander J. F. Verry, Kerry Walton, Rodney A. Hitchmough, Nic Rawlence   +4 more
openalex   +1 more source

DNA Renaturation at the Water-Phenol Interface [PDF]

, 2003
We study DNA adsorption and renaturation in a water-phenol two-phase system, with or without shaking. In very dilute solutions, single-stranded DNA is adsorbed at the interface in a salt-dependent manner.
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core   +4 more sources

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

FEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li, Pedro A. Sánchez‐Murcia, Bernd Nidetzky   +2 more
wiley   +1 more source

Optimization of DNA extraction from seeds and fresh leaf tissues of Soybean (Glycine max) [PDF]

, 2012
The effects of various components on extraction buffer such as SDS-NaCI, PVP, p-mercaptoethanol, extraction with Phenol:Chloroform:lsoamyl acetate, and incubation time on the DNA extraction from seeds and fresh leaves of Soybean (Glycine max)were ...
., Evellyn, Setiawan, Benny, Yunita, Oeke   +2 more
core  

Genomic Dna Extraction Method From Mature Leaf Of Lai (Durio Kutejensis Becc.) [PDF]

, 2016
Lai (Durio kutejensis Becc.) is an indigeneous germplasm of Kalimantan which has some superiorities compared to its close-relative, durian (Durio zibethinus Murr.). Genetic exploration of lai is important to support its breeding program.
Handayani, F. (Fitri), Murti, R. H. (Rudi), Wulandari, R. A. (Rani)   +2 more
core   +2 more sources

Can non-destructive DNA extraction of bulk invertebrate samples be used for metabarcoding?

PeerJ, 2018
Background High throughput DNA sequencing of bulk invertebrate samples or metabarcoding is becoming increasingly used to provide profiles of biological communities for environmental monitoring.
M. Carew, R. Coleman, A. Hoffmann
semanticscholar   +1 more source