Results 121 to 130 of about 4,713,939 (389)

The Challenges of DNA Extraction in Different Assorted Food Matrices: A Review.

Journal of Food Science, 2018
High-quality DNA extracts are imperative for downstream applications in molecular identification. Most processed food products undergo heat treatments causing DNA degradation, which hampers application of DNA-based techniques for food authentication ...
N. Sajali, S. Wong, U. K. Hanapi, Suhaili Abu Bakar Jamaluddin, N. A. Tasrip, M. M. Mohd Desa   +5 more
semanticscholar   +1 more source

C‐mannosylation promotes ADAMTS1 activation and secretion in human testicular germ cell tumor NEC8 cells

FEBS Letters, EarlyView.
C‐mannosylation is a unique form of protein glycosylation. In this study, we demonstrated that ADAMTS1 is C‐mannosylated at Trp562 and Trp565 in human testicular germ cell tumor NEC8 cells. We found that C‐mannosylation of ADAMTS1 is essential for its secretion, processing, enzymatic activity, and ability to promote vasculogenic mimicry. These findings
Takato Kobayashi, Takehiro Suzuki, Ryota Kawahara, Natsumi Harai, Naoshi Dohmae, Siro Simizu   +5 more
wiley   +1 more source

Optimization of DNA extraction from seeds and fresh leaf tissues of Soybean (Glycine max) [PDF]

, 2012
The effects of various components on extraction buffer such as SDS-NaCI, PVP, p-mercaptoethanol, extraction with Phenol:Chloroform:lsoamyl acetate, and incubation time on the DNA extraction from seeds and fresh leaves of Soybean (Glycine max)were ...
., Evellyn, Setiawan, Benny, Yunita, Oeke   +2 more
core  

Models and information-theoretic bounds for nanopore sequencing

, 2018
Nanopore sequencing is an emerging new technology for sequencing DNA, which can read long fragments of DNA (~50,000 bases) in contrast to most current short-read sequencing technologies which can only read hundreds of bases. While nanopore sequencers can
Diggavi, Suhas, Kannan, Sreeram, Mao, Wei   +2 more
core   +1 more source

Quantitation of intracellular NAD(P)H can monitor an imbalance of DNA single strand break repair in base excision repair deficient cells in real time [PDF]

, 2003
DNA single strand breaks (SSBs) are one of the most frequent DNA lesions in genomic DNA generated either by oxidative stress or during the base excision repair pathways.
Asakura, Shoji, Caldecott, Keith W, de Murcia, Gilbert, Hester, Susan D, Nakamura, Jun, Swenberg, James A   +5 more
core   +3 more sources

Back to Basics – The Influence of DNA Extraction and Primer Choice on Phylogenetic Analysis of Activated Sludge Communities

PLoS ONE, 2015
DNA extraction and primer choice have a large effect on the observed community structure in all microbial amplicon sequencing analyses. Although the biases are well known, no comprehensive analysis has been conducted in activated sludge communities.
M. Albertsen, S. Karst, A. S. Ziegler, R. Kirkegaard, P. Nielsen   +4 more
semanticscholar   +1 more source

The multidrug and toxin extrusion (MATE) transporter DTX51 antagonizes non‐cell‐autonomous HLS1–AMP1 signaling in a region‐specific manner

FEBS Letters, EarlyView.
The Arabidopsis mutants hls1 hlh1 and amp1 lamp1 exhibit pleiotropic developmental phenotypes. Although the functions of the causative genes remain unclear, they act in the same genetic pathway and are thought to generate non‐cell‐autonomous signals.
Takashi Nobusawa, Makoto Kusaba
wiley   +1 more source

DNA Renaturation at the Water-Phenol Interface [PDF]

, 2003
We study DNA adsorption and renaturation in a water-phenol two-phase system, with or without shaking. In very dilute solutions, single-stranded DNA is adsorbed at the interface in a salt-dependent manner.
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core   +3 more sources

An Improved Method for High Quality Metagenomics DNA Extraction from Human and Environmental Samples

Scientific Reports, 2016
To explore the natural microbial community of any ecosystems by high-resolution molecular approaches including next generation sequencing, it is extremely important to develop a sensitive and reproducible DNA extraction method that facilitate isolation ...
S. Bag, B. Saha, Ojasvi Mehta, D. Anbumani, Naveen Kumar, Mayanka Dayal, Archana Pant, Pawan Kumar, S. Saxena, K. Allin, T. Hansen, Manimozhiyan Arumugam, H. Vestergaard, O. Pedersen, Verima Pereira, P. Abraham, R. Tripathi, N. Wadhwa, S. Bhatnagar, V. G. Prakash, V. Radha, R. Anjana, V. Mohan, K. Takeda, T. Kurakawa, G. Nair, Bhabatosh Das   +26 more
semanticscholar   +1 more source

DNA extraction (BOMB) v1

, 2022
DNA extraction (BOMB)
openaire   +1 more source