Results 11 to 20 of about 371,482 (299)
Asian Journal of Andrology, 2022 We question whether, in men with an abnormal rate of sperm DNA fragmentation, the magnetic-activated cell sorting (MACS) could select spermatozoa with lower rates of DNA fragmentation as well as spermatozoa with unbalanced chromosome content ...
Sahar El Fekih +9 more
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Electroeluting DNA Fragments [PDF]
Journal of Visualized Experiments, 2010 Purified DNA fragments are used for different purposes in Molecular Biology and they can be prepared by several procedures. Most of them require a previous electrophoresis of the DNA fragments in order to separate the band of interest. Then, this band is excised out from an agarose or acrylamide gel and purified by using either: binding and elution ...
Zarzosa-Álvarez, Ana L. +3 more
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Translational Andrology and Urology, 2017 I agree with Agarwal et al. (1) that sperm DNA fragmentation (SDF) should be included in the evaluation of male factor infertility, along with semen analysis.
openaire +5 more sources
Comparison between density gradient centrifugation method, an extended version of the horizontal swim up method and the combination of both for sperm selection [PDF]
Obstetrics & Gynecology Science, 2023 Objective To compare the degree of efficiency between density gradient centrifugation (DGC) method and an extended horizontal swim-up (SU) method. Methods A total of 97 couples undergoing in vitro fertilization were enrolled in the study.
Malak Jamil +12 more
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PLoS ONE, 2021 Poor sperm morphology and an elevated DNA fragmentation level are considered to be related to spermiogenesis malfunctions as a result of genetic mutations and effects of environmental factors, including industrial pollution.
Maxim Kleshchev +2 more
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Understanding sperm DNA fragmentation [PDF]
Translational Andrology and Urology, 2017 We greatly value the commentary by Dr. Gosalvez on the “Clinical utility of sperm DNA fragmentation testing: practice recommendations based on clinical scenarios” by Agarwal et al. (1). The author initiated his well-written note with an overview of the structural and physiologic significance of sperm DNA on male reproduction addressing its ...
Majzoub, Ahmad +2 more
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DNA fragmentation in apoptosis [PDF]
Cell Research, 2000 Cleavage of chromosomal DNA into oligonucleosomal size fragments is an integral part of apoptosis. Elegant biochemical work identified the DNA fragmentation factor (DFF) as a major apoptotic endonuclease for DNA fragmentation in vitro. Genetic studies in mice support the importance of DFF in DNA fragmentation and possibly in apoptosis in vivo.
J H, Zhang, M, Xu
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DNA Fragment Conformations [PDF]
European Journal of Biochemistry, 1982 The helix‐coil transition, conformations and dynamic structures of d(A‐C‐A‐T‐G‐T) we re investigated as a function of temperature by 1H‐NMR spectroscopy at various frequencies (400, 276, 250 arid 90 MHz). Imino exchangeable protons of the d(A‐C‐A‐T‐G‐T) duplex were observed between 5°C and 25°C at 276 MHz.
Jean‐Michel NEUMANN +5 more
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Effects of Sperm DNA Fragmentation on Semen Parameters and ICSI Outcome Determined by an Improved SCD Test, Halosperm [PDF]
International Journal of Fertility and Sterility, 2010 Background Sperm DNA fragmentation is known as an important cause of male infertility. The influence of sperm DNA damage on reproductive potential has been subject of many studies indicating various results and remaining the subject controversial.
Seda Yılmaz +5 more
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Apoptosis in human unfertilized oocytes after Intracytoplasmic Sperm Injection [PDF]
, 2005 Objective To investigate the presence of programmed cell death in unfertilized oocytes after intracytoplasmic sperm injection (ICSI), assuming that previous apoptotic events could be correlated with the fertilization failure.
BOSCO, Liana +5 more
core +1 more source