Results 11 to 20 of about 373,621 (277)

Evaluation of Sperm DNA Fragmentation Using Two Different Methods: TUNEL via Fluorescence Microscopy, and Flow Cytometry

open access: yesMedicina, 2023
Background and Objectives: Sperm DNA fragmentation refers to any break in one or both of the strands of DNA in the head of a sperm. The most widely used methodologies for assessing sperm DNA fragmentation are the sperm chromatin structure assay (SCSA ...
Katerina Chatzimeletiou   +8 more
doaj   +1 more source

Sperm DNA fragmentation [PDF]

open access: yesTranslational Andrology and Urology, 2017
I agree with Agarwal et al. (1) that sperm DNA fragmentation (SDF) should be included in the evaluation of male factor infertility, along with semen analysis.
openaire   +5 more sources

MACS-annexin V cell sorting of semen samples with high TUNEL values decreases the concentration of cells with abnormal chromosomal content: a pilot study

open access: yesAsian Journal of Andrology, 2022
We question whether, in men with an abnormal rate of sperm DNA fragmentation, the magnetic-activated cell sorting (MACS) could select spermatozoa with lower rates of DNA fragmentation as well as spermatozoa with unbalanced chromosome content ...
Sahar El Fekih   +9 more
doaj   +1 more source

Comparison between density gradient centrifugation method, an extended version of the horizontal swim up method and the combination of both for sperm selection [PDF]

open access: yesObstetrics & Gynecology Science, 2023
Objective To compare the degree of efficiency between density gradient centrifugation (DGC) method and an extended horizontal swim-up (SU) method. Methods A total of 97 couples undergoing in vitro fertilization were enrolled in the study.
Malak Jamil   +12 more
doaj   +1 more source

DNA fragmentation in spermatozoa and its relationship with impaired spermatogenesis

open access: yesАндрология и генитальная хирургия, 2015
Sperm cells DNA fragmentation is one of the factors of male sub-/infertility discovered recently. At present, pathophysiological mechanisms that cause DNA fragmentation have not been studied completely.
S. A. Rudneva   +7 more
doaj   +3 more sources

Understanding sperm DNA fragmentation [PDF]

open access: yesTranslational Andrology and Urology, 2017
We greatly value the commentary by Dr. Gosalvez on the “Clinical utility of sperm DNA fragmentation testing: practice recommendations based on clinical scenarios” by Agarwal et al. (1). The author initiated his well-written note with an overview of the structural and physiologic significance of sperm DNA on male reproduction addressing its ...
Majzoub, Ahmad   +2 more
openaire   +2 more sources

DNA fragmentation in apoptosis [PDF]

open access: yesCell Research, 2000
Cleavage of chromosomal DNA into oligonucleosomal size fragments is an integral part of apoptosis. Elegant biochemical work identified the DNA fragmentation factor (DFF) as a major apoptotic endonuclease for DNA fragmentation in vitro. Genetic studies in mice support the importance of DFF in DNA fragmentation and possibly in apoptosis in vivo.
J H, Zhang, M, Xu
openaire   +2 more sources

DNA Fragment Conformations [PDF]

open access: yesEuropean Journal of Biochemistry, 1982
The helix‐coil transition, conformations and dynamic structures of d(A‐C‐A‐T‐G‐T) we re investigated as a function of temperature by 1H‐NMR spectroscopy at various frequencies (400, 276, 250 arid 90 MHz). Imino exchangeable protons of the d(A‐C‐A‐T‐G‐T) duplex were observed between 5°C and 25°C at 276 MHz.
Jean‐Michel NEUMANN   +5 more
openaire   +2 more sources

Impaired semen quality, an increase of sperm morphological defects and DNA fragmentation associated with environmental pollution in urban population of young men from Western Siberia, Russia.

open access: yesPLoS ONE, 2021
Poor sperm morphology and an elevated DNA fragmentation level are considered to be related to spermiogenesis malfunctions as a result of genetic mutations and effects of environmental factors, including industrial pollution.
Maxim Kleshchev   +2 more
doaj   +2 more sources

Apoptosis in human unfertilized oocytes after Intracytoplasmic Sperm Injection [PDF]

open access: yes, 2005
Objective To investigate the presence of programmed cell death in unfertilized oocytes after intracytoplasmic sperm injection (ICSI), assuming that previous apoptotic events could be correlated with the fertilization failure.
BOSCO, Liana   +5 more
core   +1 more source

Home - About - Disclaimer - Privacy