Results 51 to 60 of about 1,562,024 (293)
Monitoring helicase activity with molecular beacons
BioTechniques, 2008 A high-throughput, fluorescence-based helicase assay using molecular beacons is described. The assay is tested using the NS3 helicase encoded by the hepatitis C virus (HCV) and is shown to accurately monitor helicase action on both DNA and RNA.
Craig A. Belon, David N. Frick
doaj +1 more source
Identification of Escherichia coli DNA helicase IV with the use of a DNA helicase activity gel [PDF]
Journal of Bacteriology, 1989 A DNA helicase activity gel was developed based on the assumption that DNA helicases could unwind double-stranded DNA in a polyacrylamide matrix. The production of single-stranded DNA was detected by staining the activity gel with acridine orange and visualizing the gel under long-wave UV light.
V N, Trieu, D, McCarthy
openaire +2 more sources
Molecular Oncology, EarlyView.The kinase SRPK1 directly interacts with the protein TOPBP1 and regulates the pre‐mRNA splicing of WIZ thereby contributing to the activation of the ATR/CHK1 replicative checkpoint in response to replicative stress. This allows cancer cells' genomic stability and survival.
Amani Shreim +17 more
wiley +1 more source
eLife, 2016 Human DNA2 (hDNA2) contains both a helicase and a nuclease domain within the same polypeptide. The nuclease of hDNA2 is involved in a variety of DNA metabolic processes. Little is known about the role of the hDNA2 helicase. Using bulk and single-molecule
Cosimo Pinto +3 more
doaj +1 more source
Chronobiology of Cancer: How Aging Fuels Oncogenesis at the Molecular Level
Aging and Cancer, EarlyView.This graphical abstract illustrates the key biological pathways linking aging with cancer development and progression. In the upper left, cumulative exposure to ultraviolet radiation, toxins, and reactive oxygen species (ROS) causes DNA damage and genomic instability, whereas age‐related decline in repair mechanisms, such as ATM/ATR, BER, and NER ...
Anu Singh, Aroonima Misra, Sufian Zaheer
wiley +1 more source
BMC Cancer, 2019 Background The high expression of BLM (Bloom syndrome) helicase in tumors involves its strong association with cell expansion. Bisbenzylisoquinoline alkaloids own an antitumor property and have developed as candidates for anticancer drugs.
Wangming Zhang +9 more
doaj +1 more source
The mini-chromosome maintenance (Mcm) complexes interact with DNA polymerase α-primase and stimulate its ability to synthesize RNA primers. [PDF]
PLoS ONE, 2013 The Mini-chromosome maintenance (Mcm) proteins are essential as central components for the DNA unwinding machinery during eukaryotic DNA replication. DNA primase activity is required at the DNA replication fork to synthesize short RNA primers for DNA ...
Zhiying You +4 more
doaj +1 more source
Advanced Science, EarlyView.This study uncovers an unusual RNAi pathway in the rhizarian pathogen Plasmodiophora brassicae. In the absence of Dicer, a Drosha‐like RNase III protein supports the biogenesis of predominant 21‐nt small RNAs, and two Argonaute proteins mediate small RNA‐guided silencing.
Xiong Zhang +13 more
wiley +1 more source
The human Suv3 helicase interacts with replication protein A and flap endonuclease 1 in the nucleus [PDF]
, 2011 The human Suv3 helicase has been shown to be a major player in mitochondrial RNA surveillance and decay. In the present study we identify two new interaction partners of hSUV3: the RPA (replication protein A0 and FEN1 (flap endonuclease).
Stępień, Piotr P. +2 more
core +1 more source
Helicases and DNA dependent ATPases of Sulfolobus solfataricus [PDF]
, 2008 Electronic version does not contain associated previously published materialDNA is susceptible to various types of damage as a result of normal cellular metabolism or from environmental sources.
Richards, Jodi D.
core +2 more sources