Results 51 to 60 of about 2,395,071 (348)
A DNA Helicase Activity Is Associated with an MCM4, -6, and -7 Protein Complex*
Journal of Biological Chemistry, 1997 All six minichromosome maintenance (MCM) proteins have DNA-dependent ATPase motifs in the central domain which is conserved from yeast to mammals. Our group purified MCM protein complexes consisting of MCM2, -4 (Cdc21), -6 (Mis5), and -7 (CDC47) proteins
Y. Ishimi
semanticscholar +1 more source
Biomolecules, 2015 Degradation of helicases or helicase-like proteins, often mediated by ubiquitin-proteasomal pathways, plays important regulatory roles in cellular mechanisms that respond to DNA damage or replication stress.
Joshua A. Sommers +2 more
doaj +1 more source
Cell Reports, 2019 Summary: In the eukaryotic replisome, DNA unwinding by the Cdc45-MCM-Go-Ichi-Ni-San (GINS) (CMG) helicase requires a hexameric ring-shaped ATPase named minichromosome maintenance (MCM), which spools single-stranded DNA through its central channel.
Patrik Eickhoff +11 more
doaj +1 more source
Human Mitochondrial DNA Helicase TWINKLE Is Both an Unwinding and Annealing Helicase*
Journal of Biological Chemistry, 2012 Background: TWINKLE is the human mitochondrial DNA helicase associated with heritable neuromuscular diseases. Results: TWINKLE has NTPase-dependent DNA unwinding activity and NTPase-independent DNA annealing activity.
D. Sen +3 more
semanticscholar +1 more source
, 2015 DNA polymerase theta (Polθ) has been identified as a crucial alternative non-homologous end-joining factor in mammalian cells. Polθ is upregulated in a range of cancer cell types defective in homologous recombination, and knockdown has been shown to ...
Aitkenhead, H +8 more
core +1 more source
Identification of Escherichia coli DNA helicase IV with the use of a DNA helicase activity gel [PDF]
Journal of Bacteriology, 1989 A DNA helicase activity gel was developed based on the assumption that DNA helicases could unwind double-stranded DNA in a polyacrylamide matrix. The production of single-stranded DNA was detected by staining the activity gel with acridine orange and visualizing the gel under long-wave UV light.
V N, Trieu, D, McCarthy
openaire +2 more sources
Biological roles of DExH RNA helicase, RHAU [PDF]
, 2007 In this thesis, I have described the work carried out on a single protein called RHAU, dealing with aspects of protein localization in cells, the regulation of global gene expression by different mechanisms, and cellular stress-responses.
Iwamoto, Fumiko
core +1 more source
Escherichia coli DNA repair helicase Lhr is also a uracil-DNA glycosylase
, 2023 DNA glycosylases protect genetic fidelity during DNA replication by removing potentially mutagenic chemically damaged DNA bases. Bacterial Lhr proteins are well-characterized DNA repair helicases that are fused to additional 600–700 amino acids of ...
Lou‐Hing, Anna +13 more
core +1 more source
Monitoring helicase activity with molecular beacons
BioTechniques, 2008 A high-throughput, fluorescence-based helicase assay using molecular beacons is described. The assay is tested using the NS3 helicase encoded by the hepatitis C virus (HCV) and is shown to accurately monitor helicase action on both DNA and RNA.
Craig A. Belon, David N. Frick
doaj +1 more source
Subtype‐specific enhancer RNAs define transcriptional regulators and prognosis in breast cancers
Molecular Oncology, EarlyView.This study employed machine learning methodologies to perform the subtype‐specific classification of RNA‐seq data sets, which are mapped on enhancers from TCGA‐derived breast cancer patients. Their integration with gene expression (referred to as ProxCReAM eRNAs) and chromatin accessibility profiles has the potential to identify lineage‐specific and ...
Aamena Y. Patel +6 more
wiley +1 more source