Results 31 to 40 of about 1,562,024 (293)

The helicase XPD unwinds bubble structures and is not stalled by DNA lesions removed by the nucleotide excision repair pathway [PDF]

, 2010
Xeroderma pigmentosum factor D (XPD) is a 5'-3' superfamily 2 helicase and the founding member of a family of DNA helicases with iron-sulphur cluster domains.
Rouillon, Christophe, Schwarz-Linek, Ulrich, Rudolf, Jana, White, Malcolm F   +3 more
core   +1 more source

A high-throughput screen to identify novel small molecule inhibitors of the Werner Syndrome Helicase-Nuclease (WRN).

PLoS ONE, 2019
Werner syndrome (WS), an autosomal recessive genetic disorder, displays accelerated clinical symptoms of aging leading to a mean lifespan less than 50 years. The WS helicase-nuclease (WRN) is involved in many important pathways including DNA replication,
Joshua A Sommers, Tomasz Kulikowicz, Deborah L Croteau, Thomas Dexheimer, Dorjbal Dorjsuren, Ajit Jadhav, David J Maloney, Anton Simeonov, Vilhelm A Bohr, Robert M Brosh   +9 more
doaj   +1 more source

Multiple kinases inhibit origin licensing and helicase activation to ensure reductive cell division during meiosis

eLife, 2018
Meiotic cells undergo a single round of DNA replication followed by two rounds of chromosome segregation (the meiotic divisions) to produce haploid gametes.
David V Phizicky, Luke E Berchowitz, Stephen P Bell   +2 more
doaj   +1 more source

DNA helicases and their roles in cancer

DNA Repair, 2020
DNA helicases, known for their fundamentally important roles in genomic stability, are high profile players in cancer. Not only are there monogenic helicase disorders with a strong disposition to cancer, it is well appreciated that helicase variants are associated with specific cancers (e.g., breast cancer).
Srijita, Dhar, Arindam, Datta, Robert M, Brosh   +2 more
openaire   +2 more sources

ATPase mechanism of the 5'-3' DNA helicase, RecD2: evidence for a pre-hydrolysis conformation change [PDF]

, 2013
The superfamily 1 helicase, RecD2, is a monomeric, bacterial enzyme with a role in DNA repair, but with 5'-3' activity unlike most enzymes from this superfamily. Rate constants were determined for steps within the ATPase cycle of RecD2 in the presence of
Webb, Martin R, Toseland, Christopher P.
core   +1 more source

Dynamics of the Eukaryotic Replicative Helicase at Lagging-Strand Protein Barriers Support the Steric Exclusion Model

Cell Reports, 2019
Summary: Progression of DNA replication depends on the ability of the replisome complex to overcome nucleoprotein barriers. During eukaryotic replication, the CMG helicase translocates along the leading-strand template and unwinds the DNA double helix ...
Hazal B. Kose, Nicolai B. Larsen, Julien P. Duxin, Hasan Yardimci   +3 more
doaj   +1 more source

The DNA helicase Pfh1 promotes fork merging at replication termination sites to ensure genome stability [PDF]

, 2012
Bidirectionally moving DNA replication forks merge at termination sites composed of accidental or programmed DNA-protein barriers. If merging fails, then regions of unreplicated DNA can result in the breakage of DNA during mitosis, which in turn can give
Steinacher, R., Whitby, M. C., Whitby, Matthew C., Steinacher, R, Dalgaard, JZ, Osman, F., Lorenz, Alexander, Whitby, Matthew, Steinacher, Roland, Osman, F, Lorenz, A, Whitby, MC, Lorenz, A., Osman, Fekret, Dalgaard, Jacob Z.   +14 more
core   +1 more source

Sequence-dependent base pair stepping dynamics in XPD helicase unwinding

eLife, 2013
Helicases couple the chemical energy of ATP hydrolysis to directional translocation along nucleic acids and transient duplex separation. Understanding helicase mechanism requires that the basic physicochemical process of base pair separation be ...
Zhi Qi, Robert A Pugh, Maria Spies, Yann R Chemla   +3 more
doaj   +1 more source

Visualizing helicases unwinding DNA at the single molecule level. [PDF]

, 2010
DNA helicases are motor proteins that catalyze the unwinding of double-stranded DNA into single-stranded DNA using the free energy from ATP hydrolysis. Single molecule approaches enable us to address detailed mechanistic questions about how such enzymes ...
Fili, Natali, Mashanov, Gregory I, Batters, C, Dillingham, MS, Yeeles, Joseph T P, Toseland, Christopher P, Mashanov, GI, Toseland, CP, Wallace, Mark I, Webb, Martin R, Molloy, JE, Batters, Christopher, Webb, MR, Wallace, Mark, Fili, N, Dillingham, Mark S, Yeeles, JT, Molloy, Justin E   +17 more
core   +1 more source

Genetically engineered synthetic miniaturized versions of Plasmodium falciparum UvrD helicase are catalytically active. [PDF]

PLoS ONE, 2014
Helicases catalyze unwinding of double stranded nucleic acids in an energy-dependent manner. We have reported characterization of UvrD helicase from Plasmodium falciparum.
Abulaish Ansari, Mohammed Tarique, Renu Tuteja   +2 more
doaj   +1 more source