Results 21 to 30 of about 1,562,024 (293)

Helicase binding to DnaI exposes a cryptic DNA-binding site during helicase loading in Bacillus subtilis [PDF]

open access: yes, 1981
The Bacillus subtilis DnaI, DnaB and DnaD proteins load the replicative ring helicase DnaC onto DNA during priming of DNA replication. Here we show that DnaI consists of a C-terminal domain (Cd) with ATPase and DNA-binding activities and an N-terminal ...
Shi, Wei   +999 more
core   +49 more sources

DNA Helicases [PDF]

open access: yesEcoSal Plus, 2010
DNA and RNA helicases are organized into six superfamilies of enzymes on the basis of sequence alignments, biochemical data, and available crystal structures. DNA helicases, members of which are found in each of the superfamilies, are an essential group of motor proteins that unwind DNA duplexes into their component single strands in a ...
openaire   +2 more sources

Monomeric PcrA helicase processively unwinds plasmid lengths of DNA in the presence of the initiator protein RepD [PDF]

open access: yes, 2013
The helicase PcrA unwinds DNA during asymmetric replication of plasmids, acting with an initiator protein, in our case RepD. Detailed kinetics of PcrA activity were measured using bulk solution and a single-molecule imaging technique to investigate the ...
Fili, Natalia   +7 more
core   +1 more source

Cooperative base pair melting by helicase and polymerase positioned one nucleotide from each other

open access: yeseLife, 2015
Leading strand DNA synthesis requires functional coupling between replicative helicase and DNA polymerase (DNAP) enzymes, but the structural and mechanistic basis of coupling is poorly understood.
Divya Nandakumar   +2 more
doaj   +1 more source

Branching out with DNA helicases [PDF]

open access: yesCurrent Opinion in Genetics & Development, 2011
The proper resolution of branched DNA molecules, which arise during processes such as DNA replication, DNA repair, and transcription, is critical for the maintenance of the genome. Disruption of this process can lead to genome instability and cancer progression.
Timur, Yusufzai, James T, Kadonaga
openaire   +2 more sources

Structure of the Helicase Domain of DNA Polymerase Theta Reveals a Possible Role in the Microhomology-Mediated End-Joining Pathway [PDF]

open access: yes, 2015
DNA polymerase theta (Polθ) has been identified as a crucial alternative non-homologous end-joining factor in mammalian cells. Polθ is upregulated in a range of cancer cell types defective in homologous recombination, and knockdown has been shown to ...
Aitkenhead, H   +8 more
core   +1 more source

Characterisation of XPD from Sulfolobus acidocaldarius : an iron-sulphur cluster containing DNA repair helicase [PDF]

open access: yes, 2007
DNA is constantly damaged by a variety of exogenous and endogenous sources. To maintain the integrity of the genome, different DNA repair mechanisms have evolved, which deal with different kinds of DNA damage.
Rudolf, Jana
core   +2 more sources

Mammalian DNA helicase

open access: yesNucleic Acids Research, 1985
A forked DNA was constructed to serve as a substrate for DNA helicases. It contains features closely resembling a natural replication fork. The DNA was prepared in large amounts and was used to assay displacement activity during isolation from calf thymus DNA polymerases alpha holoenzyme. One form of DNA polymerase alpha holoenzyme is possibly involved
U, Hübscher, H P, Stalder
openaire   +3 more sources

Helicase Assays

open access: yesBio-Protocol, 2014
Helicases are a class of enzymes which are motor proteins using energy derived from ATP hydrolysis to move directionally along a nucliec acid phosphodiester backbone (such as DNA, RNA and DNA-RNA hybrids) and separate two annealed nucleic acid strands ...
Xin Wang   +3 more
doaj   +1 more source

Site-specific DNA Mapping of Protein Binding Orientation Using Azidophenacyl Bromide (APB)

open access: yesBio-Protocol, 2020
The orientation of a DNA-binding protein bound on DNA is determinative in directing the assembly of other associated proteins in the complex for enzymatic action.
Himasha Perera, Michael Trakselis
doaj   +1 more source

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