Results 21 to 30 of about 2,395,071 (348)

Characterize the Interaction of the DNA Helicase PriA with the Stalled DNA Replication Fork Using Atomic Force Microscopy

open access: yesBio-Protocol, 2021
In bacteria, the restart of stalled DNA replication forks requires the DNA helicase PriA. PriA can recognize and remodel abandoned DNA replication forks, unwind DNA in the 3'-to-5' direction, and facilitate the loading of the helicase DnaB onto ...
Yaqing Wang   +3 more
doaj   +1 more source

DNA Synthesis at a Fork in the Presence of DNA Helicases [PDF]

open access: yesEuropean Journal of Biochemistry, 1982
In a mixture of Escherichia coli DNA polymerase III holoenzyme, single‐strand‐binding protein, artificially forked λ bacteriophage DNA with primer annealed to the leading side of the fork, dNTPs and ATP, DNA synthesis is enhanced by helicaseII, less so by helicases I, III or rep protein of E. coli or T4 phage helicase. The effect of helicase II depends
Kuhn, B., Abdel‐Monem, M.
openaire   +3 more sources

Helicase and Polymerase Move Together Close to the Fork Junction and Copy DNA in One-Nucleotide Steps

open access: yesCell Reports, 2014
By simultaneously measuring DNA synthesis and dNTP hydrolysis, we show that T7 DNA polymerase and T7 gp4 helicase move in sync during leading-strand synthesis, taking one-nucleotide steps and hydrolyzing one dNTP per base-pair unwound/copied.
Manjula Pandey, Smita S. Patel
doaj   +1 more source

DNA Helicases

open access: yesCold Spring Harbor Symposia on Quantitative Biology, 1979
In summary, we postulate that DNA unwinding and ATP dephosphorylation are coupled in different ways, depending on whether the fibrous ATPase or one of the globular ATPases provides the catalytic agent. Unanswered is the question of whether there is stoichiometry of ATP utilization during the unwinding of a duplex, and unsolved is the role of the ...
Kuhn, B.   +2 more
openaire   +3 more sources

ATPase cycle and DNA unwinding kinetics of RecG helicase [PDF]

open access: yes, 2012
The superfamily 2 bacterial helicase, RecG, is a monomeric enzyme with a role in DNA repair by reversing stalled replication forks. The helicase must act specifically and rapidly to prevent replication fork collapse. We have shown that RecG binds tightly
Webb, Martin R   +11 more
core   +1 more source

DNA Helicases [PDF]

open access: yesEcoSal Plus, 2010
DNA and RNA helicases are organized into six superfamilies of enzymes on the basis of sequence alignments, biochemical data, and available crystal structures. DNA helicases, members of which are found in each of the superfamilies, are an essential group of motor proteins that unwind DNA duplexes into their component single strands in a ...
openaire   +2 more sources

Helicase binding to DnaI exposes a cryptic DNA-binding site during helicase loading in Bacillus subtilis [PDF]

open access: yes, 1981
The Bacillus subtilis DnaI, DnaB and DnaD proteins load the replicative ring helicase DnaC onto DNA during priming of DNA replication. Here we show that DnaI consists of a C-terminal domain (Cd) with ATPase and DNA-binding activities and an N-terminal ...
Shi, Wei   +999 more
core   +1 more source

Monomeric PcrA helicase processively unwinds plasmid lengths of DNA in the presence of the initiator protein RepD [PDF]

open access: yes, 2013
The helicase PcrA unwinds DNA during asymmetric replication of plasmids, acting with an initiator protein, in our case RepD. Detailed kinetics of PcrA activity were measured using bulk solution and a single-molecule imaging technique to investigate the ...
Fili, Natalia   +7 more
core   +1 more source

Cooperative base pair melting by helicase and polymerase positioned one nucleotide from each other

open access: yeseLife, 2015
Leading strand DNA synthesis requires functional coupling between replicative helicase and DNA polymerase (DNAP) enzymes, but the structural and mechanistic basis of coupling is poorly understood.
Divya Nandakumar   +2 more
doaj   +1 more source

Branching out with DNA helicases [PDF]

open access: yesCurrent Opinion in Genetics & Development, 2011
The proper resolution of branched DNA molecules, which arise during processes such as DNA replication, DNA repair, and transcription, is critical for the maintenance of the genome. Disruption of this process can lead to genome instability and cancer progression.
Timur, Yusufzai, James T, Kadonaga
openaire   +2 more sources

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