DNA Helicase–Polymerase Coupling in Bacteriophage DNA Replication [PDF]
Viruses, 2021 Bacteriophages have long been model systems to study the molecular mechanisms of DNA replication. During DNA replication, a DNA helicase and a DNA polymerase cooperatively unwind the parental DNA.
Chen-Yu Lo, Yang Gao
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Inhibitory effects of anthracyclines on partially purified 5′–3′ DNA helicase of Plasmodium falciparum [PDF]
Malaria Journal, 2022 Background Plasmodium falciparum has been becoming resistant to the currently used anti-malarial drugs. Searching for new drug targets is urgently needed for anti-malarial development. DNA helicases separating double-stranded DNA into single-stranded DNA
Pongruj Rattaprasert +4 more
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The Vaccinia Virus DNA Helicase Structure from Combined Single-Particle Cryo-Electron Microscopy and AlphaFold2 Prediction [PDF]
Viruses, 2022 Poxviruses are large DNA viruses with a linear double-stranded DNA genome circularized at the extremities. The helicase-primase D5, composed of six identical 90 kDa subunits, is required for DNA replication.
Stephanie Hutin +6 more
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Implications of Membrane Binding by the Fe-S Cluster-Containing N-Terminal Domain in the Drosophila Mitochondrial Replicative DNA Helicase [PDF]
Frontiers in Genetics, 2021 Recent evidence suggests that iron-sulfur clusters (ISCs) in DNA replicative proteins sense DNA-mediated charge transfer to modulate nuclear DNA replication.
Minyoung So +8 more
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History of DNA Helicases [PDF]
Genes, 2020 Since the discovery of the DNA double helix, there has been a fascination in understanding the molecular mechanisms and cellular processes that account for: (i) the transmission of genetic information from one generation to the next and (ii) the remarkable stability of the genome.
Brosh, Robert M., Matson, Steven W.
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Deinococcus radiodurans PriA is a Pseudohelicase. [PDF]
PLoS ONE, 2015 Reactivation of repaired DNA replication forks in bacteria is catalyzed by PriA helicase. This broadly-conserved bacterial enzyme can remodel the structure of DNA at a repaired DNA replication fork by unwinding small portions of duplex DNA to prepare the
Matthew E Lopper +2 more
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Primase is required for helicase activity and helicase alters the specificity of primase in the enteropathogen Clostridium difficile [PDF]
Open Biology, 2016 DNA replication is an essential and conserved process in all domains of life and may serve as a target for the development of new antimicrobials. However, such developments are hindered by subtle mechanistic differences and limited understanding of DNA ...
Erika van Eijk +8 more
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Microbiology Spectrum, 2023 DNA replication is a standard and essential function among DNA viruses; however, this functional domain's common ancestor, origin, and evolutionary path in invertebrate- and vertebrate-infecting viruses are not yet fully understood.
Perumal Arumugam Desingu +2 more
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Eukaryotic DNA helicases [PDF]
FEBS Letters, 1990 DNA is very stable in its double‐stranded form. For many processes of DNA metabolism, such as replication, repair, recombination and transcription, the DNA has to be brought transiently into a single‐stranded form. DNA helicases are enzymes capable of melting the hydrogen bonds of base pairs by using the energy of nucleoside‐5'‐triphosphate hydrolysis.
Thömmes P, Hübscher U
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Structural and mechanistic insight into DNA unwinding by Deinococcus radiodurans UvrD. [PDF]
PLoS ONE, 2013 DNA helicases are responsible for unwinding the duplex DNA, a key step in many biological processes. UvrD is a DNA helicase involved in several DNA repair pathways.
Meike Stelter +3 more
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