Results 41 to 50 of about 2,395,071 (348)

Visualizing helicases unwinding DNA at the single molecule level. [PDF]

, 2010
DNA helicases are motor proteins that catalyze the unwinding of double-stranded DNA into single-stranded DNA using the free energy from ATP hydrolysis. Single molecule approaches enable us to address detailed mechanistic questions about how such enzymes ...
Fili, Natali, Mashanov, Gregory I, Batters, C, Dillingham, MS, Yeeles, Joseph T P, Toseland, Christopher P, Mashanov, GI, Toseland, CP, Wallace, Mark I, Webb, Martin R, Molloy, JE, Batters, Christopher, Webb, MR, Wallace, Mark, Fili, N, Dillingham, Mark S, Yeeles, JT, Molloy, Justin E   +17 more
core   +1 more source

A high-throughput screen to identify novel small molecule inhibitors of the Werner Syndrome Helicase-Nuclease (WRN).

PLoS ONE, 2019
Werner syndrome (WS), an autosomal recessive genetic disorder, displays accelerated clinical symptoms of aging leading to a mean lifespan less than 50 years. The WS helicase-nuclease (WRN) is involved in many important pathways including DNA replication,
Joshua A Sommers, Tomasz Kulikowicz, Deborah L Croteau, Thomas Dexheimer, Dorjbal Dorjsuren, Ajit Jadhav, David J Maloney, Anton Simeonov, Vilhelm A Bohr, Robert M Brosh   +9 more
doaj   +1 more source

Sequence-dependent base pair stepping dynamics in XPD helicase unwinding

eLife, 2013
Helicases couple the chemical energy of ATP hydrolysis to directional translocation along nucleic acids and transient duplex separation. Understanding helicase mechanism requires that the basic physicochemical process of base pair separation be ...
Zhi Qi, Robert A Pugh, Maria Spies, Yann R Chemla   +3 more
doaj   +1 more source

Human DNA helicase HELQ participates in DNA interstrand crosslink tolerance with ATR and RAD51 paralogs

Nature Communications, 2013
Mammalian HELQ is a 3′–5′ DNA helicase with strand displacement activity. Here we show that HELQ participates in a pathway of resistance to DNA interstrand crosslinks (ICLs).
K. Takata, Shelley Reh, Junya Tomida, M. Person, R. Wood   +4 more
semanticscholar   +1 more source

Genetically engineered synthetic miniaturized versions of Plasmodium falciparum UvrD helicase are catalytically active. [PDF]

PLoS ONE, 2014
Helicases catalyze unwinding of double stranded nucleic acids in an energy-dependent manner. We have reported characterization of UvrD helicase from Plasmodium falciparum.
Abulaish Ansari, Mohammed Tarique, Renu Tuteja   +2 more
doaj   +1 more source

The Arabidopsis At1g30680 gene encodes a homologue to the phage T7 gp4 protein that has both DNA primase and DNA helicase activities

BMC Plant Biology, 2013
BackgroundThe Arabidopsis thaliana genome encodes a homologue of the full-length bacteriophage T7 gp4 protein, which is also homologous to the eukaryotic Twinkle protein. While the phage protein has both DNA primase and DNA helicase activities, in animal
J. Diray-Arce, B. Liu, J. Cupp, T. Hunt, B. Nielsen   +4 more
semanticscholar   +1 more source

Localization of an accessory helicase at the replisome is critical in sustaining efficient genome duplication [PDF]

, 2010
© The Author(s) 2010. Published by Oxford University PressGenome duplication requires accessory helicases to displace proteins ahead of advancing replication forks.
Lloyd, RG, McGlynn, P, Hazel Bell, Robert G. Lloyd, John Atkinson, Peter McGlynn, Atkinson, J, Bell, H, Christian J. Rudolph, Gupta, MK, Rudolph, CJ, Milind K. Gupta   +11 more
core   +1 more source

The Main Role of Srs2 in DNA Repair Depends on Its Helicase Activity, Rather than on Its Interactions with PCNA or Rad51

mBio, 2018
Homologous recombination (HR) is a mechanism that repairs a variety of DNA lesions. Under certain circumstances, however, HR can generate intermediates that can interfere with other cellular processes such as DNA transcription or replication.
Alex Bronstein, Lihi Gershon, Gilad Grinberg, Elisa Alonso-Perez, Martin Kupiec   +4 more
doaj   +1 more source

The molecular coupling between substrate recognition and ATP turnover in a AAA+ hexameric helicase loader

eLife, 2021
In many bacteria and eukaryotes, replication fork establishment requires the controlled loading of hexameric, ring-shaped helicases around DNA by AAA+(ATPases Associated with various cellular Activities) ATPases.
Neha Puri, Amy J Fernandez, Valerie L O'Shea Murray, Sarah McMillan, James L Keck, James M Berger   +5 more
doaj   +1 more source

Selective bypass of a lagging strand roadblock by the eukaryotic replicative DNA helicase

Cell, 2011
Summary The eukaryotic replicative DNA helicase, CMG, unwinds DNA by an unknown mechanism. In some models, CMG encircles and translocates along one strand of DNA while excluding the other strand. In others, CMG encircles and translocates along duplex DNA.
Yu V. Fu, H. Yardimci, David T. Long, T. V. Ho, Angelo Guainazzi, V. Bermudez, J. Hurwitz, Antoine M. van Oijen, O. Schärer, J. Walter   +9 more
semanticscholar   +1 more source