Results 11 to 20 of about 1,562,024 (293)
Modulation of MCM Helicase activity [PDF]
, 2011 The minichromosome maintenance (MCM) proteins are essential for the initiation and progression of DNA replication. MCMs are believed to function as the replicative helicase in archaea and eukaryotes.
Satti, Rabab
core +6 more sources
The RecQ DNA Helicases in DNA Repair [PDF]
Annual Review of Genetics, 2010 The RecQ helicases are conserved from bacteria to humans and play a critical role in genome stability. In humans, loss of RecQ gene function is associated with cancer predisposition and/or premature aging. Recent experiments have shown that the RecQ helicases function during distinct steps during DNA repair; DNA end resection, displacement-loop (D-loop)
Bernstein, Kara +2 more
openaire +3 more sources
Unwinding of Nucleosomal DNA by a DNA Helicase [PDF]
Journal of Biological Chemistry, 1995 We have asked whether a DNA helicase can unwind DNA contained within both isolated native chromatin and reconstituted chromatin containing regularly spaced arrays of nucleosome cores on a linear tandem repeat sequence. We find that Escherichia coli recBCD enzyme is capable of unwinding these DNA substrates and displacing the nucleosomes, although both ...
A K, Eggleston +3 more
openaire +2 more sources
Mutations altering the interplay between GkDnaC helicase and DNA reveal an insight into helicase unwinding. [PDF]
PLoS ONE, 2011 Replicative helicases are essential molecular machines that utilize energy derived from NTP hydrolysis to move along nucleic acids and to unwind double-stranded DNA (dsDNA).
Yu-Hua Lo +4 more
doaj +1 more source
Bio-Protocol, 2021 In bacteria, the restart of stalled DNA replication forks requires the DNA helicase PriA. PriA can recognize and remodel abandoned DNA replication forks, unwind DNA in the 3'-to-5' direction, and facilitate the loading of the helicase DnaB onto ...
Yaqing Wang +3 more
doaj +1 more source
Life Science Alliance, 2021 WRN is a key drug target for cancers with microsatellite instability. A crystal structure of the helicase domain of WRN identifies its unique features and provides a basis for structure-guided design of inhibitors. Loss of WRN, a DNA repair helicase, was
Joseph A Newman +12 more
doaj +1 more source
ATPase cycle and DNA unwinding kinetics of RecG helicase [PDF]
, 2012 The superfamily 2 bacterial helicase, RecG, is a monomeric enzyme with a role in DNA repair by reversing stalled replication forks. The helicase must act specifically and rapidly to prevent replication fork collapse. We have shown that RecG binds tightly
Webb, Martin R +11 more
core +1 more source
Cell Reports, 2014 By simultaneously measuring DNA synthesis and dNTP hydrolysis, we show that T7 DNA polymerase and T7 gp4 helicase move in sync during leading-strand synthesis, taking one-nucleotide steps and hydrolyzing one dNTP per base-pair unwound/copied.
Manjula Pandey, Smita S. Patel
doaj +1 more source
Cold Spring Harbor Symposia on Quantitative Biology, 1979 In summary, we postulate that DNA unwinding and ATP dephosphorylation are coupled in different ways, depending on whether the fibrous ATPase or one of the globular ATPases provides the catalytic agent. Unanswered is the question of whether there is stoichiometry of ATP utilization during the unwinding of a duplex, and unsolved is the role of the ...
Kuhn, B. +2 more
openaire +3 more sources
DNA Synthesis at a Fork in the Presence of DNA Helicases [PDF]
European Journal of Biochemistry, 1982 In a mixture of Escherichia coli DNA polymerase III holoenzyme, single‐strand‐binding protein, artificially forked λ bacteriophage DNA with primer annealed to the leading side of the fork, dNTPs and ATP, DNA synthesis is enhanced by helicaseII, less so by helicases I, III or rep protein of E. coli or T4 phage helicase. The effect of helicase II depends
Kuhn, B., Abdel‐Monem, M.
openaire +3 more sources