Results 241 to 250 of about 47,217 (278)
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Biochemistry, 1988
The DNA helicase activity of DNA-dependent ATPase B purified from mouse FM3A cells [Seki, M., Enomoto, T., Hanaoka, F., & Yamada, M. (1987) Biochemistry 26, 2924-2928] has been further characterized. The helicase activity was assayed with partially duplex DNA substrates in which oligonucleotides to be released by the enzyme were radiolabeled ...
M, Seki +4 more
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The DNA helicase activity of DNA-dependent ATPase B purified from mouse FM3A cells [Seki, M., Enomoto, T., Hanaoka, F., & Yamada, M. (1987) Biochemistry 26, 2924-2928] has been further characterized. The helicase activity was assayed with partially duplex DNA substrates in which oligonucleotides to be released by the enzyme were radiolabeled ...
M, Seki +4 more
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Helicases, G4‐DNAs, and Drug Design
ChemMedChem, 2014AbstractNew helicase assays that recognise therapeutically important G4‐DNA structures will lead to the discovery of novel molecular entities that bind not only to G4‐tetrads, but also to grooves and loops of G4‐DNA. Such assays can also provide inhibitors of G4‐specific helicases that will shed light on the emerging involvement of helicases in cancer ...
Tracy K, Hale +3 more
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XPB: An unconventional SF2 DNA helicase
Progress in Biophysics and Molecular Biology, 2015XPB is a 3'-5' DNA helicase belonging to the superfamily 2 (SF2) of helicases. XPB is an essential core subunit of the eukaryotic basal transcription factor complex TFIIH which plays a dual role in transcription and DNA repair: 1) to facilitate the melting of the promoter during the initiation of RNA polymerase II transcription; 2) to unwind double ...
Li, Fan, Kevin T, DuPrez
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Fluorometric assays for characterizing DNA helicases
Methods, 2010DNA helicases belong to an important class of motor proteins and are involved in almost all aspects of DNA metabolism. They hydrolyze NTP to translocate along ssDNA and unwind dsDNA by relying on chemical to physical energy transfer processes that are achieved via nucleotide-state-dependent conformational changes.
Dou, Shuo-Xing, Xi, Xu Guang
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MECHANISMS OF HELICASE-CATALYZED DNA UNWINDING
Annual Review of Biochemistry, 1996DNA helicases are essential motor proteins that function to unwind duplex DNA to yield the transient single-stranded DNA intermediates required for replication, recombination, and repair. These enzymes unwind duplex DNA and translocate along DNA in reactions that are coupled to the binding and hydrolysis of 5′-nucleoside triphosphates (NTP).
T M, Lohman, K P, Bjornson
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[DNA helicases and human diseases].
Medecine sciences : M/S, 2006DNA helicases are molecular motors that catalyse the unwinding of energetically unstable structures into single strands and have therefore an essential role in nearly all metabolism transactions. Defects in helicase function can result in human syndromes in which predisposition to cancer and genomic instability are common features.
Uhring, Muriel, Poterszman, Arnaud
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DNA Helicases of Escherichia coli
1991A great deal has been learned in the last 15 years with regard to how helicase enzymes participate in DNA metabolism and how they interact with their DNA substrates. However, many questions remain unanswered. Of critical importance is an understanding of how NTP hydrolysis and hydrogen-bond disruption are coupled.
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DNA Helicases and DNA Motor Proteins
2012Preface.- Overview: What are DNA helicases?.- Structure and mechanisms of SF1 DNA helicases.- Structure and mechanisms of SF2 DNA helicases.- Structure and mechanisms of hexameric helicases.- Helicases at the replication fork.- DNA helicases associated with genetic instability, cancer and aging.- The Helicase-primase complex as a target for effective ...
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DNA helicases in inherited human disorders
Current Opinion in Genetics & Development, 1997Six known or predicted helicases that are mutated in human syndromes are now recognized. These syndromes include xeroderma pigmentosum, Cockayne's syndrome, trichothiodystrophy, Bloom's syndrome, Werner's syndrome, and alpha-thalassemia mental retardation on the X chromosome.
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