Mcm10 promotes rapid isomerization of CMG-DNA for replisome bypass of lagging strand DNA blocks
eLife, 2017 Replicative helicases in all cell types are hexameric rings that unwind DNA by steric exclusion in which the helicase encircles the tracking strand only and excludes the other strand from the ring.
Lance D Langston +7 more
doaj +1 more source
Structural Basis of DEAH/RHA Helicase Activity
Crystals, 2017 DEAH/RHA helicases are members of a large group of proteins collectively termed DExH-box, which also include Ski2-like and NS3/NPH-II helicases. By binding and remodeling DNA and RNA, DEAH/RHA helicases play critical roles in many cellular processes ...
Michael C. Chen +1 more
doaj +1 more source
Machines on Genes: Enzymes that Make, Break and Move DNA and RNA [PDF]
, 2010 As the vital information repositories of the cell, the nucleic acids DNA and RNA pose many challenges as enzyme substrates. To produce, maintain and repair DNA and RNA, and to extract the genetic information that they encode, a battery of remarkable ...
Bates +13 more
core +1 more source
Unwinding of Nucleosomal DNA by a DNA Helicase [PDF]
Journal of Biological Chemistry, 1995 We have asked whether a DNA helicase can unwind DNA contained within both isolated native chromatin and reconstituted chromatin containing regularly spaced arrays of nucleosome cores on a linear tandem repeat sequence. We find that Escherichia coli recBCD enzyme is capable of unwinding these DNA substrates and displacing the nucleosomes, although both ...
A K, Eggleston +3 more
openaire +2 more sources
Molecular characteristics of reiterative DNA unwinding by the Caenorhabditis elegans RecQ helicase [PDF]
, 2019 The RecQ family of helicases is highly conserved both structurally and functionally from bacteria to humans. Defects in human RecQ helicases are associated with genetic diseases that are characterized by cancer predisposition and/or premature aging. RecQ
Ahn, Byungchan +3 more
core +1 more source
Homology sensing via non-linear amplification of sequence-dependent pausing by RecQ helicase
eLife, 2019 RecQ helicases promote genomic stability through their unique ability to suppress illegitimate recombination and resolve recombination intermediates. These DNA structure-specific activities of RecQ helicases are mediated by the helicase-and-RNAseD like C-
Yeonee Seol +3 more
doaj +1 more source
Arabidopsis RecQl4A suppresses homologous recombination and modulates DNA damage responses [PDF]
, 2005 The DNA damage response and DNA recombination are two interrelated mechanisms involved in maintaining the integrity of the genome, but in plants they are poorly understood.
Bachrati +64 more
core +3 more sources
RPA alleviates the inhibitory effect of vinylphosphonate internucleotide linkages on DNA unwinding by BLM and WRN helicases [PDF]
, 2017 Bloom (BLM) and Werner (WRN) syndrome proteins are members of the RecQ family of SF2 DNA helicases. In this paper, we show that restricting the rotational DNA backbone flexibility, by introducing vinylphosphonate internucleotide linkages in the ...
Bradley, G. +5 more
core
Structural biology of ferritin nanocages
FEBS Letters, EarlyView.Ferritin is a conserved iron‐storage protein that sequesters iron as a ferric mineral core within a nanocage, protecting cells from oxidative damage and maintaining iron homeostasis. This review discusses ferritin biology, structure, and function, and highlights recent cryo‐EM studies revealing mechanisms of ferritinophagy, cellular iron uptake, and ...
Eloise Mastrangelo, Flavio Di Pisa
wiley +1 more source
Structure and primase-mediated activation of a bacterial dodecameric replicative helicase [PDF]
, 2015 International audienceReplicative helicases are essential ATPases that unwind DNA to initiate chromosomal replication. While bacterial replicative DnaB helicases are hexameric, Helicobacter pylori DnaB (HpDnaB) was found to form double hexamers, similar ...
Bazin, Alexandre +4 more
core +4 more sources