Results 31 to 40 of about 1,489,376 (287)
Branching out with DNA helicases [PDF]
Current Opinion in Genetics & Development, 2011 The proper resolution of branched DNA molecules, which arise during processes such as DNA replication, DNA repair, and transcription, is critical for the maintenance of the genome. Disruption of this process can lead to genome instability and cancer progression.
Timur, Yusufzai, James T, Kadonaga
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Nucleic Acids Research, 1985 A forked DNA was constructed to serve as a substrate for DNA helicases. It contains features closely resembling a natural replication fork. The DNA was prepared in large amounts and was used to assay displacement activity during isolation from calf thymus DNA polymerases alpha holoenzyme. One form of DNA polymerase alpha holoenzyme is possibly involved
U, Hübscher, H P, Stalder
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DNA Helicase–Polymerase Coupling in Bacteriophage DNA Replication [PDF]
Viruses, 2021 Bacteriophages have long been model systems to study the molecular mechanisms of DNA replication. During DNA replication, a DNA helicase and a DNA polymerase cooperatively unwind the parental DNA. By surveying recent data from three bacteriophage replication systems, we summarized the mechanistic basis of DNA replication by helicases and polymerases ...
Chen-Yu Lo, Yang Gao
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ATPase mechanism of the 5'-3' DNA helicase, RecD2: evidence for a pre-hydrolysis conformation change [PDF]
, 2013 The superfamily 1 helicase, RecD2, is a monomeric, bacterial enzyme with a role in DNA repair, but with 5'-3' activity unlike most enzymes from this superfamily. Rate constants were determined for steps within the ATPase cycle of RecD2 in the presence of
Webb, Martin R, Toseland, Christopher P.
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Frontiers in Molecular Biosciences, 2022 Maintaining genomic integrity is critical for sustaining individual animals and passing on the genome to subsequent generations. Several enzymes, such as DNA helicases and DNA polymerases, are involved in maintaining genomic integrity by unwinding and ...
Masaoki Kohzaki
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Localization of an accessory helicase at the replisome is critical in sustaining efficient genome duplication [PDF]
, 2010 © The Author(s) 2010. Published by Oxford University PressGenome duplication requires accessory helicases to displace proteins ahead of advancing replication forks.
Lloyd, RG +11 more
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Quantitative and kinetic single-molecule analysis of DNA unwinding by Escherichia coli UvrD helicase
Biophysics and Physicobiology, 2022 Helicases are nucleic acid-unwinding enzymes involved in the maintenance of genome integrity. Helicases share several “helicase motifs” that are highly conserved amino acid sequences and are classified into six superfamilies (SFs).
Hiroaki Yokota
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Structure of the Helicase Domain of DNA Polymerase Theta Reveals a Possible Role in the Microhomology-Mediated End-Joining Pathway [PDF]
, 2015 DNA polymerase theta (Polθ) has been identified as a crucial alternative non-homologous end-joining factor in mammalian cells. Polθ is upregulated in a range of cancer cell types defective in homologous recombination, and knockdown has been shown to ...
Aitkenhead, H +8 more
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Unwinding and Rewinding: Double Faces of Helicase?
Journal of Nucleic Acids, 2012 Helicases are enzymes that use ATP-driven motor force to unwind double-stranded DNA or RNA. Recently, increasing evidence demonstrates that some helicases also possess rewinding activity—in other words, they can anneal two complementary single-stranded ...
Yuliang Wu
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DNA helicases and their roles in cancer
DNA Repair, 2020 DNA helicases, known for their fundamentally important roles in genomic stability, are high profile players in cancer. Not only are there monogenic helicase disorders with a strong disposition to cancer, it is well appreciated that helicase variants are associated with specific cancers (e.g., breast cancer).
Srijita, Dhar +2 more
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