Results 91 to 100 of about 817,746 (315)

COMP–PMEPA1 axis promotes epithelial‐to‐mesenchymal transition in breast cancer cells

Molecular Oncology, EarlyView.
This study reveals that cartilage oligomeric matrix protein (COMP) promotes epithelial‐to‐mesenchymal transition (EMT) in breast cancer. We identify PMEPA1 (protein TMEPAI) as a novel COMP‐binding partner that mediates EMT via binding to the TSP domains of COMP, establishing the COMP–PMEPA1 axis as a key EMT driver in breast cancer.
Konstantinos S. Papadakos, Gilar Gorji‐bahri, Lejla Gradjan, Julia Zajac, Kacper Gil, Yvonne Thokozile Nduku, Anna M. Blom   +6 more
wiley   +1 more source

The extreme C terminus of herpes simplex virus DNA polymerase is crucial for functional interaction with processivity factor UL42 and for viral replication

, 1993
The herpes simplex virus DNA polymerase is composed of two subunits, a large catalytic subunit (Pol) and a smaller subunit (UL42) that increases the processivity of the holoenzyme.
Bebrin, William R, Paul Digard, M. Coen, Digard, Paul, Weisshart, Klaus, Klaus Weisshart, Coen, Donald M, William R. Bebrin, Donald   +8 more
core  

Comparison of Different DNA Isolation Methods from Grapevine (Vitis vinifera) Leaves

Separations
The extraction of high-quality DNA is essential for molecular analyses in grapevine, yet differences among commonly used protocols remain underexplored.
Nina Buljević, Darko Preiner, Iva Šikuten, Ivana Tomaz   +3 more
doaj   +1 more source

Rapid Isolation of PCR-Ready DNA from Blood, Bone Marrow and Cultured Cells, Based on Paramagnetic Beads

BioTechniques, 1997
PCR-based methods for the analysis of genomic DNA are becoming increasingly common both in research and for routine purposes. A rapid, small-scale DNA isolation method is needed to take full advantage of the speed and automation potential of the PCR ...
Arne Deggerdal, Frank Larsen
doaj   +1 more source

Pre‐analytical optimization of cell‐free DNA and extracellular vesicle‐derived DNA for mutation detection in liquid biopsies

Molecular Oncology, EarlyView.
Pre‐analytical handling critically determines liquid biopsy performance. This study defines practical best‐practice conditions for cell‐free DNA (cfDNA) and extracellular vesicle–derived DNA (evDNA), showing how processing time, storage conditions, tube type, and plasma input volume affect DNA integrity and mutation detection.
Jonas Dohmen, Lucrezia De Santis, Bret M. Stephens, Vincent Bernard, Jörg C. Kalff, Lara Braun, Phillipp Leyendecker, Johannes Röttgen, Daniel Weissinger, Anirban Maitra, Paola A. Guerrero, Alexander Semaan   +11 more
wiley   +1 more source

Application of genetic and evolutionary approaches in marine conservation and management (Case study: PANGAS project)

Scientific Annals of the Danube Delta Institute, 2016
Contemporary studies of population structure and genetic diersity increasingly employ new analytical and technical tools that proide high-resolution genetic information. These tools, that include procedures such as polymerase chain reaction amplification
TOIC Katarina
doaj   +1 more source

EDNRB‐dependent endothelin signaling reduces proliferation and promotes proneural‐to‐mesenchymal transition in gliomas

Molecular Oncology, EarlyView.
Glioma cells mainly express the endothelin receptor EDNRB, while EDNRA is restricted to a perivascular tumor subpopulation. Endothelin signaling reduces glioma cell proliferation while promoting migration and a proneural‐to‐mesenchymal transition associated with poor prognosis. This pathway activates Ca2+, K+, ERK, and STAT3 signalings and is regulated
Donovan Pineau, Leonor Garcia, Hugo Arnold, Antonija Hanžek, Maialen Arrieta, Laurent R Gauthier, Christine Granotier‐Beckers, François D Boussin, Amaury Herbet, Marie Hautière, Valentin Asei‐Ceschino, Clémentin Jacques, Laura Brard, Thomas Harnois, Valérie Coronas, Bruno Constantin, Aurélien Chatelier, Jean Chemin, Serge Urbach, Martial Seveno, Szimonetta Hideg, Chantal Ripoll, Kasandra Aguilar‐Cázarez, Min Zheng, Guo‐Hao Huang, Sheng‐Qing Lv, Lei Zhang, Philippe Rondard, Laurent Prezeau, Jean‐Philippe Pin, Hugues Duffau, Luc Bauchet, Valérie Rigau, Franck Denat, Charles Truillet, Didier Boquet, Jean‐Philippe Hugnot   +36 more
wiley   +1 more source

Desenvolvimento de um protocolo de isolamento de cloroplastos e DNA plastidial em coníferas e sequenciamento do genoma plastidial de Podocarpus lambertii Klotzch ex Endl. [PDF]

, 2014
Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Agrárias, Programa de Pós-Graduação em Recursos Genéticos Vegetais, Florianópolis, 2014.O sequenciamento do genoma plastidial de coníferas vem sendo realizado por meio do
Vieira, Leila do Nascimento
core  

Genome-Wide Mapping of DNA Methylation 5mC by Methylated DNA Immunoprecipitation (MeDIP)-Sequencing

, 2020
Methylated DNA immunoprecipitation is a large scale purification technique. It enables the isolation of methylated DNA fragments for subsequent locus-specific or genome-wide analysis.
Ben Maamar, Millissia, Millissia Ben Maamar, Michael K. Skinner, Daniel Beck, Ingrid Sadler-Riggleman, Beck, Daniel, Skinner, Michael K., Sadler-Riggleman, Ingrid   +7 more
core   +1 more source

Pathological replication in cells lacking RecG DNA translocase [PDF]

, 2009
This is an open access article, shared under a Creative Commons licence. Copyright © 2009 The Authors.Little is known about what happens when forks meet to complete DNA replication in any organism.
Lloyd, RG, Robert G. Lloyd, Lynda Harris, Harris, L, Christian J. Rudolph, Amy L. Upton, Rudolph, CJ, Upton, AL   +7 more
core   +1 more source