Results 71 to 80 of about 817,746 (315)

Rab14 regulates the transport of human papillomavirus to the trans‐Golgi network for infectious cell entry

open access: yesFEBS Letters, EarlyView.
This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley   +1 more source

Circular DNA's from HeLa cell nuclei and mitochondria

open access: yes, 1970
An electron microscopic observation was made on the DNA's extracted from purified HeLa cell nuclei, mitochondria, and the whole cell, and fractionated by ethidium bromide-cesium chloride density gradient method or sucrose density gradient method. Nuclear
Oda, Takuzo   +4 more
core   +1 more source

Comparison of DNA Extraction Methods Between Conventional, Kit, Alkali and Buffer-Only for PCR Amplification on Raw and Boiled Bovine and Porcine Meat

open access: yesJournal of Experimental Life Science, 2017
Detection of porcine contamination in food material by employing PCR techniques is integral in halal food confirmation. However, PCR is both costly and laborious, particularly in DNA isolation method.
Arif Yahya   +3 more
doaj   +1 more source

The ubiquitin‐proteasome system and autophagy as guardians of the cellular proteome

open access: yesFEBS Letters, EarlyView.
This Perspective covers the three principles governing the crosstalk between the ubiquitin‐proteasome system and autophagy in cellular proteostasis: (1) a shared ubiquitin code routing substrates via shuttle factors or autophagy receptors; (2) spatial compartmentalization into phase‐separated degradation hubs and organelle‐specific modules (exemplified
Ivan Dikic
wiley   +1 more source

First successful DNA isolation and profiling from bone using an approach that is non-destructive toward bone surface

open access: yes, 2016
Commonly used destructive DNA isolation techniques consist of pulverization which leads to the complete destruction of smaller bones or irreversible damage to larger bones through the cutting of extensive fragments.
Tadeusz Dobosz   +3 more
core   +1 more source

Reversal of terminal differentiation and control of DNA replication [PDF]

open access: yes, 1993
DNA replication in mammalian cells occurs in discrete nuclear foci. Here we show that terminally differentiated myotubes can be induced to reenter S phase and show the same pattern of replication foci as cycling cells.
Cardoso, M. Cristina   +2 more
core   +1 more source

An unexpected alternative viologen electron mediator site in tungsten‐containing formate dehydrogenase

open access: yesFEBS Letters, EarlyView.
An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley   +1 more source

Cloning strategies for the isolation of an a-amylase inhibitor/endochitinase gene from the seeds of Coix lachryma-jobi [PDF]

open access: yes, 1993
The aim of this work was to isolate the gene encoding a bifunctional a-amylase inhibitor/endochitinase protein from the seeds of Coix lachryma-jobi, a tropical cereal.
Fairweather, Donna, Fairweather, D
core  

Optimalization of DNA isolation from oral epithelial mucous cell with smear method

open access: yesPadjadjaran Journal of Dentistry, 2009
Deoxyribonucleic acid (DNA) is a genetic material which is found in all living organisms. On the human cell or eukaryotes cell, the DNA is found in the nucleus cell and the mitochondria. The DNA arrangement on each cell in human body is the same, that is
Saskia L. Nasroen   +2 more
doaj   +1 more source

Isolation and Quantification of Extracellular DNA from Biofluids

open access: yesBio-Protocol, 2020
Extracellular DNA is studied as a diagnostic biomarker, but also as a factor involved in the pathophysiology of several diseases due to its pro-inflammatory properties.
Ľubica Janovičová   +3 more
doaj   +1 more source

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