Results 191 to 200 of about 817,746 (315)

Isolation Of Plasmid Dna

, 2014
Author: Saji Menon ### Reagents and solutions 1. Alkaline Solution I - 50 mM glucose - 25 mM Tris HCl (pH 8.0) - 10 mM EDTA (pH 8.0) - 10 mg/ml lysozyme - Alkaline Solution II - 0.2 N NaOH (2.0 ml of 10 N NaOH in 100ml double distilled water) - 1.0% SDS - Alkaline Solution III - 5 M Potassium acetate - Glacial acetic acid - Autoclaved
openaire   +3 more sources

Decreased cold‐sensing function of the transient receptor potential channel TRPM8 from tailed amphibians

FEBS Open Bio, EarlyView.
Despite frogs avoiding low temperatures, examination of four salamander species revealed that none avoided cold and all possessed cold tolerance. Functional analysis of TRPM8, a cold sensor, showed that all salamander TRPM8s had lost their cold sensitivity.
Tadahiro Sawao, Daiki Kobayashi, Shogo Hori, Osamu Saitoh   +3 more
wiley   +1 more source

Investigation and optimization of DNA isolation efficiency using ferrite-based magnetic nanoparticles. [PDF]

Biotechnol Rep (Amst)
Gerzsenyi TB, Ilosvai ÁM, Kristály F, Daróczi L, Owen MC, Viskolcz B, Vanyorek L, Szőri-Dorogházi E.   +7 more
europepmc   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

‘The Farm Beneath the Sand’ – an archaeological case study on ancient ‘dirt’ DNA

, 2009
It is probable that ‘The Farm Beneath the Sand’ will come to stand for a revolution in archaeological investigation. The authors show that a core of soil from an open field can provide a narrative of grazing animals, human occupation and their departure,
Heyn, P., Gilbert, Thomas, Hebsgaard, M.B., Pedersen, Jette Arneborg, Schweger, C., Allentoft, M.E., Willerslev, E., Heyn, Patricia, Allentoft, M., Allentoft, Morten Erik, Schweger, Charles, Gilbert, Tom, Willerslev, Eske, Hebsgaard, Martin B., Munch, K., Munch, Kasper, Hebsgaard, Martin Bay, Hebsgaard, M., Arneborg, Jette, Gilbert, M. Thomas P., Arneborg, J., Allentoft, Morten E., Bunce, M., Bunce, Michael, Gilbert, T.P.   +24 more
core  

Tissue-specific DNA isolation from dissected millipedes for nanopore sequencing. [PDF]

Biol Methods Protoc
Cruz E, Wittstock W, Snyder BA, Sengupta A.   +3 more
europepmc   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Magnetic Nanoparticle-Based DNA Isolation from Refined Soybean Oil: A Cost-Effective Approach for GM Testing. [PDF]

Foods
Xia Y, Guo M, Liu K, Xin Y, Chen F.
europepmc   +1 more source

Comparative Evaluation of an Easy Laboratory Method for the Concentration of Oocysts and Commercial DNA Isolation Kits for the Molecular Detection of Cyclospora cayetanensis in Silt Loam Soil Samples. [PDF]

Microorganisms, 2022
Shipley A, Arida J, Almeria S.
europepmc   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source