Results 201 to 210 of about 817,746 (315)

Real-time PCR quantitation of hepatitis B virus DNA using automated sample preparation and murine cytomegalovirus internal control

, 2005
Quantitation of circulating hepatitis B virus (HBV) DNA is important for monitoring disease progression and for assessing the response to antiviral therapy. Several commercial and 'in house' assays for HBV DNA quantitation have been described but many of
Tedder, RS, Ayliffe, U, Grant, PR, Ferns, RB, Garson, JA   +4 more
core  

Protocol for mitochondrial DNA isolation from Macrobrachium rosenbergii and Penaeus monodon using differential centrifugation. [PDF]

STAR Protoc
Pal DC, Karim MM.
europepmc   +1 more source

An optimized high-quality DNA isolation protocol for <i>spodoptera frugiperda</i> J. E. smith (Lepidoptera: Noctuidae). [PDF]

MethodsX, 2021
Marín DV, Castillo DK, López-Lavalle LAB, Chalarca JR, Pérez CR.   +4 more
europepmc   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

Standardized Culture of Skin Fibroblasts From Punch Biopsies for Germline DNA Isolation in Myeloid Malignancies: A Practical Bedside-to-Laboratory Approach. [PDF]

Bio Protoc
Kour P, Kumari N, Kaushal N, Sharma T, Naseem S, Binota J, Sachdeva MS, Jain A, Rohilla M, Das R, Malhotra P, Rastogi P.   +11 more
europepmc   +1 more source

Blood‐based proteomic profiling reveals context‐dependent changes in BCL2‐associated signaling during taxane therapy in breast cancer patients

FEBS Open Bio, EarlyView.
Chemotherapy side effects significantly impact cancer survivors' quality of life. Using protein levels in blood samples from breast cancer patients before and after 12 weeks of taxane treatment, we detected treatment‐dependent changes in calcium signaling and aging pathways associated with cancer recurrence.
Saira Munshani, Eiman Y. Ibrahim, Rozalyn L. Rodwin, Leah M. Ferrucci, Kim Blenman, Maryam Lustberg, Barbara E. Ehrlich   +6 more
wiley   +1 more source

DNA repair in bacteroides fragilis

, 2008
Includes abstract.Includes bibliographical leaves (leaves 89-101).Bacteroides fragilis is a gut commensal in both humans and animals where it benefits the host through metabolizing indigestible compounds, stimulating the immune system and protecting ...
Steffens, Laura Sione
core  

Comparison of Three DNA Isolation Methods and Two Sequencing Techniques for the Study of the Human Microbiota. [PDF]

Life (Basel)
Plaza-Díaz J, Fernández MF, García F, Chueca N, Fontana L, Álvarez-Mercado AI.   +5 more
europepmc   +1 more source

Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis

FEBS Open Bio, EarlyView.
Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho, Rebecca Schmitt, Aneesha Dasgupta, Alexandra Ducharme, Jason Doles   +4 more
wiley   +1 more source

A highly cost-effective, eco-friendly tissue lysis and extraction method for faster DNA isolation from fish fin. [PDF]

PLoS One
Lenka P, Singh N, Ghosh D, Mahato V, Ghosh S.   +4 more
europepmc   +1 more source