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2003
Blood samples for most coagulation tests are collected into 3.8% trisodium citrate in a ratio of 1 part anticoagulant to 9 parts blood. Whole-blood samples for DNA isolation can be stored at -50°C and the DNA prepared at a later stage. A more convenient method requiring less freezer space is to store buffy coats-the interface between the red cells and ...
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Blood samples for most coagulation tests are collected into 3.8% trisodium citrate in a ratio of 1 part anticoagulant to 9 parts blood. Whole-blood samples for DNA isolation can be stored at -50°C and the DNA prepared at a later stage. A more convenient method requiring less freezer space is to store buffy coats-the interface between the red cells and ...
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Isolation of DNA Fragments for Microinjection
2003The purification of a DNA fragment for microinjection is extremely important. This chapter describes a rapid and efficient technique for isolating specific DNA fragments from agarose gels run in Tris-acetate buffer, and was first described by Vogelstein and Gillespie.
Smith, D.R., Murphy, D.
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Analytical Biochemistry, 1991
A rapid, economical method of DNA isolation from blood was developed that yields DNA suitable for Southern analysis and polymerase chain reactions without organic solvent extractions. Bovine DNA was prepared from peripheral leukocytes and nuclei using pronase E digestion and ethanol precipitation.
T L, Kendall, D J, Byerley, R, Dean
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A rapid, economical method of DNA isolation from blood was developed that yields DNA suitable for Southern analysis and polymerase chain reactions without organic solvent extractions. Bovine DNA was prepared from peripheral leukocytes and nuclei using pronase E digestion and ethanol precipitation.
T L, Kendall, D J, Byerley, R, Dean
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The Isolation of Mitochondria and Mitochondrial DNA
1986Publisher Summary This chapter describes the isolation procedures for obtaining mitochondrial DNA (mtDNA) from three tissue types of several genera of dicot plants. It discusses the isolation of suspension culture mitochondria capable of active in organello protein synthesis.
Maureen R. Hanson +4 more
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Caulimovirus Isolation and DNA Extraction
1998Members of the cauhmovn-us group (1) each have a circular double-stranded DNA genome of approx 8 kbp that is encapsidated m a spherical, naked nucleocapsid of approx 50 nm diameter (Fig. 1). Caulimovnuses charactertsttcally produce subcellular mclusron bodies m infected tissues that contam most of the virions found in cells, embedded m an apparently ...
S N, Covey +3 more
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Analytical Biochemistry, 1989
Methods are described that allow DNA to be prepared from widely different yeasts (Candida utilis, Saccharomyces cerevisiae, and Schizosaccharomyces pombe). The methods are reliably reproducible, and the DNA obtained is of appropriate quality for the construction of gene libraries (upper limit of size range consistently 50-150 kbp).
W, Mann, J, Jeffery
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Methods are described that allow DNA to be prepared from widely different yeasts (Candida utilis, Saccharomyces cerevisiae, and Schizosaccharomyces pombe). The methods are reliably reproducible, and the DNA obtained is of appropriate quality for the construction of gene libraries (upper limit of size range consistently 50-150 kbp).
W, Mann, J, Jeffery
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Dodecylamine in the isolation of bacterial DNA
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1971Abstract Lysis of enterobacteria by lysozyme and detergent was greatly accelerated by n-dodecylamine. Nicking of supercoiled DNA was not detected.
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Cold Spring Harbor Protocols, 2018
This method is used to isolate yeast genomic DNA or shuttle plasmids that replicated both in Saccharomyces cerevisiae and in E. coli.
Michael R, Green, Joseph, Sambrook
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This method is used to isolate yeast genomic DNA or shuttle plasmids that replicated both in Saccharomyces cerevisiae and in E. coli.
Michael R, Green, Joseph, Sambrook
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Cold Spring Harbor Protocols, 2009
INTRODUCTIONMany different DNA isolation methods have been employed successfully in ants. Parameters such as the size and developmental stage of the specimen (egg, larvae, or adult) and the subsequent use of the DNA will mostly determine which method should be used. Ant body sizes range from minute (1-2 mm in length) to large (30 mm), and the volume of
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INTRODUCTIONMany different DNA isolation methods have been employed successfully in ants. Parameters such as the size and developmental stage of the specimen (egg, larvae, or adult) and the subsequent use of the DNA will mostly determine which method should be used. Ant body sizes range from minute (1-2 mm in length) to large (30 mm), and the volume of
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Isolating Microsatellite DNA Loci
2005A series of techniques are presented to construct genomic DNA libraries highly enriched for microsatellite DNA loci. The individual techniques used here derive from several published protocols but have been optimized and tested in our research laboratories as well as in classroom settings at the University of South Carolina and University of Georgia ...
Travis C, Glenn, Nancy A, Schable
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