Results 281 to 290 of about 817,746 (315)

Recombinant DNA and gene isolation

Trends in Cardiovascular Medicine, 1993
Although a formidable array of approaches fall under the broad umbrella of molecular genetics, the basic technology is elegant and conceptually simple (Watson et al. 1983, Roberts et al. 1992). It has enabled a reductionist approach to be applied to the study of genes and their sequences, as well as the transcripts that underlie organ development and ...
openaire   +2 more sources

Isolation of Recombinant DNA Elongation Proteins

2009
This chapter summarizes isolation procedures of four recombinant human proteins crucial for DNA replication: (a) the replicative DNA polymerase (pol) delta, (b) proliferating cell nuclear antigen (PCNA), (c) replication protein A (RP-A), and (d) replication factor C (RF-C).
van Loon, B, Ferrari, E, Hübscher, U
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Isolation of Genomic DNA

1987
Within the scope of this laboratory guide it is impossible to give a full review of all the procedures used in the isolation of intact genomic DNA. However, we shall describe a general method we used which gives an excellent quality of both nuclei and DNA suitable for genomic sequencing.
Hanspeter Saluz, Jean-Pierre Jost
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Isolation and characterization of DNA-DNA and DNA-RNA.

Biochemistry, 1977
A simple method for the isolation and characterization of DNA-DNA and DNA-RNA hybrid molecules formed in solution was developed. It was based on the fact that, in appropriate salt concentration, such as 5% Na2HPO4, DNA in either double-stranded (DNA-DNA or DNA-RNA) or single-stranded forms, but not free nucleotides, can bind to ...
S, Salzberg, Z, Levi, M, Aboud, A, Goldberger   +3 more
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Yeast DNA Isolation: Midiprep

Cold Spring Harbor Protocols, 2006
David C, Amberg, Daniel J, Burke, Jeffrey N, Strathern   +2 more
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Isolation and Purification of DNA

2011
Deoxyribonucleic acid (DNA) isolation and purification are essential techniques for molecular biology studies. The quality and integrity of the DNA isolated directly affects the results of all subsequent scientific research.1–4 A number of methods have been developed for extraction and purification of DNA from different tissue types and organisms.
Leland Cseke, Chuanfu An, Chung-Jui Tsai
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Isolation of Kinetoplast DNA

2003
T A, Shapiro, V A, Klein, P T, Englund
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Rapid Isolation of Mammalian DNA

Cold Spring Harbor Protocols, 2006
Joseph, Sambrook, David W, Russell
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Isolation of Mitochondrial DNA

2003
G R, Bignell, A R, Miller, I H, Evans
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Classification of Isolates by DNA-DNA Hybridization

1998
K, Sachse, H, Hotzel
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