Frontiers in Plant Science, 2015 The DNA double-strand break (DSB) is a critical type of damage, and can be induced by both endogenous sources (e.g. errors of oxidative metabolism, transposable elements, programmed meiotic breaks, or perturbation of the DNA replication fork) and ...
Tomoyuki eFurukawa +3 more
doaj +1 more source
Human DNA ligases in replication and repair
DNA Repair, 2020 To ensure genome integrity, the joining of breaks in the phosphodiester backbone of duplex DNA is required during DNA replication and to complete the repair of almost all types of DNA damage. In human cells, this task is accomplished by DNA ligases encoded by three genes, LIG1, LIG3 and LIG4.
Annahita Sallmyr +4 more
openaire +4 more sources
DNA Ligase III is critical for mtDNA integrity but not Xrcc1-mediated nuclear DNA repair
Nature, 2011 DNA replication and repair in mammalian cells involves three distinct DNA ligases: ligase I (Lig1), ligase III (Lig3) and ligase IV (Lig4). Lig3 is considered a key ligase during base excision repair because its stability depends upon its nuclear binding
Yankun Gao +6 more
semanticscholar +1 more source
DNA‑Directed Assembly of Photonic Nanomaterials for Diagnostic and Therapeutic Applications
Advanced Materials, EarlyView.DNA‐directed assembly offers a powerful strategy for constructing structured photonic nanomaterials with precise spatial control. This review provides a comprehensive overview of recent advancements in DNA‐assembled photonic nanomaterials for diagnostics and therapeutics, highlighting key design principles, functionalization strategies, and optical ...
Longjiang Ding +5 more
wiley +1 more source
Efficient assembly of very short oligonucleotides using T4 DNA Ligase
BMC Research Notes, 2010 Background In principle, a pre-constructed library of all possible short oligonucleotides could be used to construct many distinct gene sequences. In order to assess the feasibility of such an approach, we characterized T4 DNA Ligase activity on short ...
Holt Robert A +2 more
doaj +1 more source
FUS Selectively Facilitates circRNAs Packing into Small Extracellular Vesicles within Hypoxia Neuron
Advanced Science, EarlyView.This study aimed to unravel the role of FUS in the loading of functional circRNAs into neuronal sEVs under hypoxic conditions, which involves their aggregations in SGs and specific interaction between the FUS Zf_RanBP domain to regulate circRNA sorting in sEVs.
Jiankun Zang +14 more
wiley +1 more source
The decrease in Rad51 and DNA ligase IV nuclear protein expression in Msh2 knockdown HC11 cells induced the low CRISPR/Cas9-mediated knock-in efficiency at the β-casein gene locus [PDF]
Animal BioscienceObjective Successful gene editing technology is crucial in molecular biology and related fields. An essential part of an efficient knock-in system is increasing homologous recombination (HR) efficiency in the double-strand break (DSB) repair pathways ...
Ga-Yeon Kim, Man-Jong Kang
doaj +1 more source
DNA damage induces Yap5-dependent transcription of ECO1/CTF7 in Saccharomyces cerevisiae.
PLoS ONE, 2020 Yeast Eco1 (ESCO2 in humans) acetyltransferase converts chromatin-bound cohesins to a DNA tethering state, thereby establishing sister chromatid cohesion.
Michael G Mfarej, Robert V Skibbens
doaj +1 more source
Replication of proto-RNAs sustained by ligase-helicase cycle in oligomer world [PDF]
arXiv, 2013 A mechanism of the replication of proto-RNAs in oligomer world is proposed. The replication is carried out by a minimum cycle which is sustained by a ligase and a helicase. We expect that such a cycle actually worked in the primordial soup and can be constructed in vitro.
arxiv
Human DNA ligase III bridges two DNA ends to promote specific intermolecular DNA end joining [PDF]
, 2015 Mammalian DNA ligase III (LigIII) functions in both nuclear and mitochondrial DNA metabolism. In the nucleus, LigIII has functional redundancy with DNA ligase I whereas LigIII is the only mitochondrial DNA ligase and is essential for the survival of ...
Ellenberger, Tom +5 more
core +2 more sources