Results 11 to 20 of about 337,677 (206)

Structures of LIG1 that engage with mutagenic mismatches inserted by polβ in base excision repair

Nature Communications, 2022
Ligase I seals two ends of DNA during DNA repair and replication. Here — solving structures of Ligase I with imperfect DNA harboring mismatches incorporated by DNA polymerase — the authors reveal how the repair proteins maintain fidelity at final ...
Qun Tang, Mitchell Gulkis, Robert McKenna, Melike Çağlayan   +3 more
doaj   +1 more source

High-fidelity DNA ligation enforces accurate Okazaki fragment maturation during DNA replication

Nature Communications, 2021
DNA ligase 1 (LIG1) finalizes eukaryotic nuclear DNA synthesis by sealing Okazaki fragments using DNA end-joining reactions. Here the authors, by studying an engineered low-fidelity LIG1, reveal that LIG1 is a highly accurate DNA ligase in vivo.
Jessica S. Williams, Percy P. Tumbale, Mercedes E. Arana, Julian A. Rana, R. Scott Williams, Thomas A. Kunkel   +5 more
doaj   +1 more source

Differential requirement for BRCA1-BARD1 E3 ubiquitin ligase activity in DNA damage repair and meiosis in the Caenorhabditis elegans germ line.

PLoS Genetics, 2023
The tumor suppressor BRCA1-BARD1 complex regulates many cellular processes; of critical importance to its tumor suppressor function is its role in genome integrity.
Qianyan Li, Arshdeep Kaur, Kyoko Okada, Richard J McKenney, JoAnne Engebrecht   +4 more
doaj   +1 more source

Thermostable DNA ligases from hyperthermophiles in biotechnology

Frontiers in Microbiology, 2023
DNA ligase is an important enzyme ubiquitous in all three kingdoms of life that can ligate DNA strands, thus playing essential roles in DNA replication, repair and recombination in vivo.
Jingru Shi, Philippe M. Oger, Peng Cao, Likui Zhang   +3 more
doaj   +1 more source

SUMO chain formation is required for response to replication arrest in S. pombe [PDF]

, 2009
SUMO is a ubiquitin-like protein that is post-translationally attached to one or more lysine residues on target proteins. Despite having only 18% sequence identity with ubiquitin, SUMO contains the conserved betabetaalphabetabetaalphabeta fold present in
A Gorg, A Pichler, Andrew Skilton, B Xhemalce, Brenda Mercer, C Hoege, CM Pickart, E Harlow, EA Andrews, Emily Outwin, F al-Khodairy, Felicity Z. Watts, FZ Watts, GR Bylebyl, Gustavo Goldman, H Sun, HD Ulrich, HL Klein, I Matic, J Bahler, J Prudden, JC Ho, JC Ho, Jenny C. Y. Ho, JR Mullen, K Tanaka, KP Bencsath, M Rechsteiner, M Shayeghi, MH Tatham, MH Tatham, O Kerscher, P Bayer, P Stelter, PL Kannouche, RJ Sims 3rd, RT Hay, RT Hay, S Vijay-Kumar, T Caspari, V Lallemand-Breitenbach, Y Takahashi   +41 more
core   +12 more sources

PARP-3 and APLF function together to accelerate nonhomologous end joining [PDF]

, 2010
PARP-3 is a member of the ADP-ribosyl transferase superfamily of unknown function. We show that PARP-3 is stimulated by DNA double-strand breaks (DSBs) in vitro and functions in the same pathway as the poly (ADP-ribose)-binding protein APLF to accelerate
Ahel, Ame, Anna E.O. Fisher, Audebert, Bekker-Jensen, Benjamin, Bernardo Reina-San-Martin, Boulton, Brown, Bryant, Bryant, Caldecott, Caldecott, Critchlow, Ding, Drouet, El-Khamisy, Farmer, Fisher, Grawunder, Guy Poirier, Hochegger, Hottiger, Iles, Isabelle Robert, Kanno, Keith W. Caldecott, Kickhoefer, Kleine, Le Page, Lehtio, Limei Ju, Lobrich, Loseva, Macrae, Mahaney, Maria C. Zuma, Mehrotra, Michele Rouleau, Morrison, Okano, Otto, Poirier, Robert, Roberts, Rogakou, Rogakou, Rouleau, Rulten, Smith, Soulas-Sprauel, Stevnsner, Stuart L. Rulten, Sugimura, Trucco, Wang, Yan, Yang, Yano, Yano   +59 more
core   +1 more source

Identification of the DNA repair defects in a case of Dubowitz syndrome. [PDF]

PLoS ONE, 2013
Dubowitz Syndrome is an autosomal recessive disorder with a unique set of clinical features including microcephaly and susceptibility to tumor formation. Although more than 140 cases of Dubowitz syndrome have been reported since 1965, the genetic defects
Jingyin Yue, Huimei Lu, Shijie Lan, Jingmei Liu, Mark N Stein, Bruce G Haffty, Zhiyuan Shen   +6 more
doaj   +1 more source

Impact of DNA ligase IV on the fidelity of end joining in human cells [PDF]

, 2003
A DNA ligase IV (LIG4)‐null human pre‐B cell line and human cell lines with hypomorphic mutations in LIG4 are significantly impaired in the frequency and fidelity of end joining using an in vivo plasmid assay.
Baldeyron, Celine, Jeggo, Penny, Kysela, B, Lieber, Michael R, Manolis, Kostas, Masson, Christel, Papadopoulo, Dora, Riballo, Enriqueta, Smith, Julianne   +8 more
core   +2 more sources

Evaluation of NAD(+)-dependent DNA ligase of mycobacteria as a potential target for antibiotics [PDF]

, 2007
Mycobacteria contain genes for several DNA ligases, including ligA, which encodes a NAD+-dependent enzyme that has been postulated to be a target for novel antibacterial compounds.
Bowater, Richard P., Brzostek, Anna, Dziadek, Jarosław, Korycka-Machala, Malgorzata, Rumijowska-Galewicz, Anna, Rychta, Ewelina, Sayer, Heather R.   +6 more
core   +2 more sources

DNA ligase 1 deficient plants display severe growth defects and delayed repair of both DNA single and double strand breaks

BMC Plant Biology, 2009
Background DNA ligase enzymes catalyse the joining of adjacent polynucleotides and as such play important roles in DNA replication and repair pathways. Eukaryotes possess multiple DNA ligases with distinct roles in DNA metabolism, with clear differences ...
Bray Clifford M, Provost Claire M, Kozak Jaroslav, Waterworth Wanda M, Angelis Karel J, West Christopher E   +5 more
doaj   +1 more source