Results 31 to 40 of about 350,926 (202)

Label-free electrochemical monitoring of DNA ligase activity [PDF]

, 2008
This study presents a simple, label-free electrochemical technique for the monitoring of DNA ligase activity. DNA ligases are enzymes that catalyze joining of breaks in the backbone of DNA and are of significant scientific interest due to their essential
Ahel I., Bellamy S. R., Borodina T. A., Bullard D. R., Cahova-Kucharikova K., Cao W. G., Doherty A. J., Fadrna R., Fojta M., Fojta M., Fojta M., Fojta M., Fojta M., Fojta M., Friedberg E. C., Gowers D. M., Heiter D. F., Kucharikova K., Liu L. F., Palecek E., Pascal J. M., Rass U., Schrock R. D., Scott B. O. S., Shuman S., Tomkinson A. E., Vacek J., Wilkinson A., Wilkinson A., Zauner G.   +29 more
core   +1 more source

PARP-3 and APLF function together to accelerate nonhomologous end joining [PDF]

, 2010
PARP-3 is a member of the ADP-ribosyl transferase superfamily of unknown function. We show that PARP-3 is stimulated by DNA double-strand breaks (DSBs) in vitro and functions in the same pathway as the poly (ADP-ribose)-binding protein APLF to accelerate
Ahel, Ame, Anna E.O. Fisher, Audebert, Bekker-Jensen, Benjamin, Bernardo Reina-San-Martin, Boulton, Brown, Bryant, Bryant, Caldecott, Caldecott, Critchlow, Ding, Drouet, El-Khamisy, Farmer, Fisher, Grawunder, Guy Poirier, Hochegger, Hottiger, Iles, Isabelle Robert, Kanno, Keith W. Caldecott, Kickhoefer, Kleine, Le Page, Lehtio, Limei Ju, Lobrich, Loseva, Macrae, Mahaney, Maria C. Zuma, Mehrotra, Michele Rouleau, Morrison, Okano, Otto, Poirier, Robert, Roberts, Rogakou, Rogakou, Rouleau, Rulten, Smith, Soulas-Sprauel, Stevnsner, Stuart L. Rulten, Sugimura, Trucco, Wang, Yan, Yang, Yano, Yano   +59 more
core   +1 more source

DNA ligase 1 deficient plants display severe growth defects and delayed repair of both DNA single and double strand breaks

BMC Plant Biology, 2009
Background DNA ligase enzymes catalyse the joining of adjacent polynucleotides and as such play important roles in DNA replication and repair pathways. Eukaryotes possess multiple DNA ligases with distinct roles in DNA metabolism, with clear differences ...
Bray Clifford M, Provost Claire M, Kozak Jaroslav, Waterworth Wanda M, Angelis Karel J, West Christopher E   +5 more
doaj   +1 more source

A case report of an adolescent with ligase-4 deficiency and the potential dangers of ionizing radiation in this rare patient population

Radiology Case Reports, 2021
DNA ligase IV deficiency is a rare disorder characterized by mutations in the LIG4 gene. Mutations in this gene cause a wide array of phenotypes, many of which are fatal early in life. We present an adolescent patient with heterozygous LIG4 mutations and
Priyal Chadha, BA, Ryan Thibodeau, MD, MPH, Abtin Jafroodifar, MD, Anand Majmudar, MD   +3 more
doaj   +1 more source

NAD+-dependent DNA ligases of Mycobacterium tuberculosis and Streptomyces coelicolor [PDF]

, 2003
Sequencing of the genomes of Mycobacterium tuberculosis H37Rv and Streptomyces coelicolor A3(2) identified putative genes for an NAD+-dependent DNA ligase. We have cloned both open reading frames and overexpressed the protein products in Escherichia coli.
Bowater, Richard, Bullard, Desmond, Day, Jonathan, Kieser, Tobias, Sayer, Heather, Smith, Andrew, Wilkinson, Adam   +6 more
core   +1 more source

Analysis of ligation and DNA binding by Escherichia coli DNA ligase (LigA). [PDF]

, 2005
NAD+-dependent DNA ligases are essential enzymes in bacteria, with the most widely studied of this class of enzymes being LigA from Escherichia coli. NAD+-dependent DNA ligases comprise several discrete structural domains, including a BRCT domain at the ...
Adam Wilkinson, Alexandra Bonner, Altschul, Andrew Hemmings, Andrew Smith, Baer, Bork, Brotz-Oesterhelt, Callebaut, Derbyshire, Dermody, Desmond Bullard, Doherty, Doherty, Doherty, Feng, Gajiwala, Georlette, Georlette, Glover, Gong, Grawunder, Guex, Gunther, Halligan, Heather Sayer, Herrmann, Huyton, Jeon, Joo, Kaczmarek, Kodama, Krishnan, Lavesa-Curto, Lee, Legrain, Lehman, Manuel Lavesa-Curto, Masson, Odell, Pascal, Richard Bowater, Sambrook, Shuman, Singleton, Sriskanda, Sriskanda, Sriskanda, Sriskanda, Subramanya, Timson, Timson, Wieland, Wilkinson, Wilkinson, Williams, Yamane, Yamane, Zhang, Zimmerman   +59 more
core   +1 more source

Ribonucleolytic resection is required for repair of strand displaced nonhomologous end-joining intermediates [PDF]

, 2013
Nonhomologous end-joining (NHEJ) pathways repair DNA double-strand breaks (DSBs) in eukaryotes and many prokaryotes, although it is not reported to operate in the third domain of life, archaea. Here, we describe a complete NHEJ complex, consisting of DNA
Bartlett, Edward, Brissett, Nigel C, Doherty, Aidan J   +2 more
core   +1 more source

Detection of ligation products of DNA linkers with 5'-OH ends by denaturing PAGE silver stain. [PDF]

PLoS ONE, 2012
To explore if DNA linkers with 5'-hydroxyl (OH) ends could be joined by commercial T4 and E. coli DNA ligase, these linkers were synthesized by using the solid-phase phosphoramidite method and joined by using commercial T4 and E. coli DNA ligases.
Feng Gao, Huafu Zhou, Wei Li, Xuerong Zhang   +3 more
doaj   +1 more source

Structure and Function of a Mycobacterial NHEJ DNA Repair Polymerase [PDF]

, 2006
Non homologous end-joining (NHEJ)-mediated repair of DNA double-strand breaks in prokaryotes requires Ku and a specific multidomain DNA ligase (LigD).
Aidan J. Doherty, Angel J. Picher, Arezi, Augustin, Bowater, Chen, Chen, Critchlow, Daley, Darren Thompson, DeFazio, Della, Dominguez, Emsley, Garcia-Diaz, Gavin C. Fox, Gong, Gong, Ito, Iyer, Jones, Krejci, La Fortelle, Lao-Sirieix, Lao-Sirieix, Lipps, Luis Blanco, Ma, Ma, Mahajan, Matsumoto, McCoy, Murshudov, Nick McElhinney, Nick McElhinny, Nick McElhinny, Nigel C. Brissett, Paula Andrade, Paull, Picher, Pitcher, Pitcher, Potterton, Potterton, Powell, Prasad, Ramsden, Raquel Juarez, Robert S. Pitcher, Ruiz, Rydberg, Singhal, Steitz, Tseng, Weller, Wilson, Winn, Wu, Zhang, Zhu   +59 more
core   +1 more source

The phage T4 DNA ligase in vivo improves the survival-coupled bacterial mutagenesis

Microbial Cell Factories, 2019
Background Microbial mutagenesis is an important avenue to acquire microbial strains with desirable traits for industry application. However, mutagens either chemical or physical used often leads narrow library pool due to high lethal rate.
Junshu Wang, Fapeng Liu, Tianyuan Su, Yizhao Chang, Qi Guo, Qian Wang, Quanfeng Liang, Qingsheng Qi   +7 more
doaj   +1 more source