Bacteriophage origin of some minimal ATP-dependent DNA ligases: a new structure from Burkholderia pseudomallei with striking similarity to Chlorella virus ligase [PDF]
Scientific Reports, 2021 DNA ligases, the enzymes responsible for joining breaks in the phosphodiester backbone of DNA during replication and repair, vary considerably in size and structure.
Jolyn Pan +4 more
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Crystal Structure of an ATP-Dependent DNA Ligase from Bacteriophage T7 [PDF]
Cell, 1996 The crystal structure of the ATP-dependent DNA ligase from bacteriophage T7 has been solved at 2.6 A resolution. The protein comprises two domains with a deep cleft running between them. The structure of a complex with ATP reveals that the nucleotide binding pocket is situated on the larger N-terminal domain, at the base of the cleft between the two ...
Aidan J Doherty, Dale B Wigley
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Enzyme–adenylate structure of a bacterial ATP-dependent DNA ligase with a minimized DNA-binding surface [PDF]
Acta Crystallographica Section D: Biological Crystallography, 2014 DNA ligases are a structurally diverse class of enzymes which share a common catalytic core and seal breaks in the phosphodiester backbone of double-stranded DNAviaan adenylated intermediate. Here, the structure and activity of a recombinantly produced ATP-dependent DNA ligase from the bacteriumPsychromonassp. strain SP041 is described.
Adele Williamson +2 more
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Characterization of an ATP-dependent DNA ligase encoded by Haemophilus influenzae [PDF]
Nucleic Acids Research, 1997 We report that Haemophilus influenzae encodes a 268 amino acid ATP-dependent DNA ligase. The specificity of Haemophilus DNA ligase was investigated using recombinant protein produced in Escherichia coli. The enzyme catalyzed efficient strand joining on a singly nicked DNA in the presence of magnesium and ATP (Km = 0.2 microM).
Stewart Shuman
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T4 DNA ligase structure reveals a prototypical ATP-dependent ligase with a unique mode of sliding clamp interaction [PDF]
Nucleic Acids Research, 2018 DNA ligases play essential roles in DNA replication and repair. Bacteriophage T4 DNA ligase is the first ATP-dependent ligase enzyme to be discovered and is widely used in molecular biology, but its structure remained unknown. Our crystal structure of T4 DNA ligase bound to DNA shows a compact α-helical DNA-binding domain (DBD), nucleotidyl-transferase
Ke Shi, Heather O Bohl, Surajit Banerjee
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Recombinant expression and purification of an ATP-dependent DNA ligase from Aliivibrio salmonicida [PDF]
Protein Expression and Purification, 2014 The genome of the psychrophilic fish-pathogen Aliivibrio salmonicida encodes a putative ATP-dependent DNA ligase in addition to a housekeeping NAD-dependent enzyme. In order to study the structure and activity of the ATP dependent ligase in vitro we have undertaken its recombinant production and purification from an Escherichia coli based expression ...
Adele Williamson
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Characterization of an ATP-dependent DNA ligase from the thermophilic archaeon Methanobacterium thermoautotrophicum [PDF]
Nucleic Acids Research, 2000 We report the production, purification and characterization of a DNA ligase encoded by the thermophilic archaeon Methanobacterium thermoautotrophicum. The 561 amino acid MTH: ligase catalyzed strand-joining on a singly nicked DNA in the presence of a divalent cation (magnesium, manganese or cobalt) and ATP (K(m) 1.1 microM). dATP can substitute for ATP,
Stewart Shuman
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A role for the ATP-dependent DNA ligase lig E of Neisseria gonorrhoeae in biofilm formation
BMC MicrobiologyBackground The ATP-dependent DNA ligase Lig E is present as an accessory DNA ligase in numerous proteobacterial genomes, including many disease-causing species.
Jolyn Pan +4 more
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Structures of ATP-bound DNA ligase D in a closed domain conformation reveal a network of amino acid and metal contacts to the ATP phosphates [PDF]
Journal of Biological Chemistry, 2019 DNA ligases are the sine qua non of genome integrity and essential for DNA replication and repair in all organisms. DNA ligases join 3'-OH and 5'-PO4 ends via a series of three nucleotidyl transfer steps. In step 1, ligase reacts with ATP or NAD+ to form a covalent ligase-(lysyl-Nζ)-AMP intermediate and release pyrophosphate (PPi) or nicotinamide ...
Stewart Shuman
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Crystal structure of ATP-dependent DNA ligase from Rhizobium phage vB_RleM_P10VF. [PDF]
Acta Crystallogr F Struct Biol CommunDNA ligases are foundational molecular-biological tools used for cloning and sequencing workflows, and are essential replicative enzymes for all cellular life forms as well as many viruses and bacteriophage. There is considerable interest in structurally and functionally characterizing novel DNA ligases and profiling their suitability for molecular ...
Rothweiler U, Leiros HKS, Williamson A.
europepmc +3 more sources