Results 21 to 30 of about 77,074 (235)
Biology Direct, 2009 Background Eukaryotic Nucleo-Cytoplasmic Large DNA Viruses (NCLDV) encode most if not all of the enzymes involved in their DNA replication. It has been inferred that genes for these enzymes were already present in the last common ancestor of the NCLDV ...
Koonin Eugene V, Yutin Natalya
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Characterization of an ATP-dependent DNA ligase from the acidophilic archaeon “Ferroplasma acidarmanus” Fer1 [PDF]
Extremophiles, 2006 Analysis of the genome of "Ferroplasma acidarmanus" Fer1, an archaeon that is an extreme acidophile, identified an open reading frame encoding a putative ATP-dependent DNA ligase, which we termed FaLig. The deduced amino acid sequence of FaLig contains 595 amino acids, with a predicted molecular mass of 67.8 kDa. "F.
Jackson, Brian R. +4 more
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Characterization of a Thermophilic ATP-Dependent DNA Ligase from the Euryarchaeon Pyrococcus horikoshii [PDF]
Journal of Bacteriology, 2005 ABSTRACT Archaea encode a DNA ligase composed of a C-terminal catalytic domain typical of ATP-dependent ligases plus an N-terminal domain similar to that found in eukaryotic cellular and poxvirus DNA ligases. All archaeal DNA ligases characterized to date have ATP-dependent adenylyltransferase and nick-joining activities. However,
Niroshika, Keppetipola, Stewart, Shuman
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ATPase-dependent control of the Mms21 SUMO ligase during DNA repair.
PLoS Biology, 2015 Modification of proteins by SUMO is essential for the maintenance of genome integrity. During DNA replication, the Mms21-branch of the SUMO pathway counteracts recombination intermediates at damaged replication forks, thus facilitating sister chromatid ...
Marcelino Bermúdez-López +11 more
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Bioinformatic analysis of Tetrahymena thermophila ATP dependent DNA ligases
New Biotechnology, 2009 …
Akdamar, H. Acelya +2 more
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Molecules, 2017 The DNA ligases, enzymes that seal breaks in the backbones of DNA, are essential for all organisms, however bacterial ligases essential for DNA replication use β-nicotinamide adenine dinucleotide as their co-factor, whereas those that are essential in ...
Malgorzata Korycka-Machala +6 more
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Design and Synthesis of Ag Nanocluster Molecular Beacon for Adenosine Triphosphate Detection
Journal of Analytical Methods in Chemistry, 2019 This study presents a fluorescence method for detecting adenosine triphosphate (ATP) based on a label-free Ag nanocluster molecular beacon (MB) with high sensitivity.
Xiaoshuang Li +7 more
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Biochemical Pharmacology, 2008 T4 DNA ligase and the ubiquitin activating enzyme (E1), catalyze the synthesis of ATP beta,gamma-bisphosphonate derivatives. Concerning T4 DNA ligase: (i) etidronate (pC(OH)(CH(3))p) displaced the AMP moiety of the complex E-AMP in a concentration dependent manner; (ii) the K(m) values and the rate of synthesis k(cat) (s(-1)), determined for the ...
Günther Sillero, María A. +3 more
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A Primer-dependent Polymerase Function of Pseudomonas aeruginosa ATP-dependent DNA Ligase (LigD) [PDF]
Journal of Biological Chemistry, 2005 Pseudomonas aeruginosa encodes two putative DNA ligases: a classical NAD(+)-dependent DNA ligase (LigA) plus an ATP-dependent DNA ligase (LigD). LigD exemplifies a family of bacterial proteins that consist of a ligase domain fused to flanking domains that resemble nucleases and/or polymerases. Here we purify LigD and show that it possesses an intrinsic
Hui, Zhu, Stewart, Shuman
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The eukaryotic Pso2/Snm1/Artemis proteins and their function as genomic and cellular caretakers
Brazilian Journal of Medical and Biological Research, 2005 DNA double-strand breaks (DSBs) represent a major threat to the genomic stability of eukaryotic cells. DNA repair mechanisms such as non-homologous end joining (NHEJ) are responsible for the maintenance of eukaryotic genomes.
D. Bonatto +3 more
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