Genome biology, 2002 By catalyzing the joining of breaks in the phosphodiester backbone of duplex DNA, DNA ligases play a vital role in the diverse processes of DNA replication, recombination and repair. Three related classes of ATP-dependent DNA ligase are readily apparent in eukaryotic cells.
Ina V, Martin, Stuart A, MacNeill
core +4 more sources
Characterization of a Baculovirus-Encoded ATP-Dependent DNA Ligase [PDF]
Journal of Virology, 1998 ABSTRACTSequence analysis of theLymantria disparmulticapsid nucleopolyhedrovirus (LdMNPV) genome identified an open reading frame (ORF) encoding a 548-amino-acid (62-kDa) protein that showed 35% amino acid sequence identity with vaccinia virus ATP-dependent DNA ligase. Ligase homologs have not been reported from other baculoviruses.
M N, Pearson, G F, Rohrmann
openaire +2 more sources
The minimal Bacillus subtilis nonhomologous end joining repair machinery. [PDF]
PLoS ONE, 2013 It is widely accepted that repair of double-strand breaks in bacteria that either sporulate or that undergo extended periods of stationary phase relies not only on homologous recombination but also on a minimal nonhomologous end joining (NHEJ) system ...
Miguel de Vega
doaj +1 more source
Identification of Novel Inhibitors of Escherichia coli DNA Ligase (LigA)
Molecules, 2021 Present in all organisms, DNA ligases catalyse the formation of a phosphodiester bond between a 3′ hydroxyl and a 5′ phosphate, a reaction that is essential for maintaining genome integrity during replication and repair.
Arqam Alomari +10 more
doaj +1 more source
The Transcriptional Response to DNA-Double-Strand Breaks in Physcomitrella patens. [PDF]
PLoS ONE, 2016 The model bryophyte Physcomitrella patens is unique among plants in supporting the generation of mutant alleles by facile homologous recombination-mediated gene targeting (GT).
Yasuko Kamisugi +2 more
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Use of ATP, dATP and their α-thio derivatives to study DNA ligase adenylation [PDF]
Biochemical Journal, 1990 Bacteriophage-T4 and human type I DNA ligases were found capable of self-adenylating upon exposure to both ribo- and deoxyribo-[alpha-35S]thio-ATP. However, the joining reaction does not take place in the presence of the deoxyribotriphosphates. Enzyme adenylation is reversed in all cases by an excess of PPi, but the rate of reversion is lower with thio
A Montecucco +4 more
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Attomolar Detection of HIV-1 With Label-Free RCA-rCRISPR on Smartphone. [PDF]
Adv Sci (Weinh)A new diagnostic strategy integrating rolling circle amplification (RCA) with a plasmid reporter–based ratiometric CRISPR (rCRISPR) system was developed to detect unfragmented HIV genomes with single‐digit attomolar sensitivity. The method quantifies targets using the plasmid relaxation ratio and employs a smartphone‐based mini‐gel device to enable ...
Mohammad N +12 more
europepmc +2 more sources
Template-directed ligation of tethered mononucleotides by t4 DNA ligase for kinase ribozyme selection. [PDF]
PLoS ONE, 2010 BACKGROUND: In vitro selection of kinase ribozymes for small molecule metabolites, such as free nucleosides, will require partition systems that discriminate active from inactive RNA species.
David G Nickens +3 more
doaj +1 more source
Characterization of an ATP-dependent DNA ligase from the thermophilic archaeon Methanobacterium thermoautotrophicum [PDF]
Nucleic Acids Research, 2000 We report the production, purification and characterization of a DNA ligase encoded by the thermophilic archaeon Methanobacterium thermoautotrophicum. The 561 amino acid MTH: ligase catalyzed strand-joining on a singly nicked DNA in the presence of a divalent cation (magnesium, manganese or cobalt) and ATP (K(m) 1.1 microM). dATP can substitute for ATP,
V, Sriskanda +3 more
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PprA contributes to Deinococcus radiodurans resistance to nalidixic acid, genome maintenance after DNA damage and interacts with deinococcal topoisomerases. [PDF]
PLoS ONE, 2014 PprA is known to contribute to Deinococcus radiodurans' remarkable capacity to survive a variety of genotoxic assaults. The molecular bases for PprA's role(s) in the maintenance of the damaged D.
Swathi Kota +4 more
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