Carboxymethylcytosine is a natural base modification and a handle for bacteriophage DNA hypermodification [PDF]
Nature CommunicationsBacteriophages possess a wide array of DNA modifications, with many acting as molecular camouflage to evade host immune defenses. Sequence databases contain numerous bacteriophage enzymes of unknown function, with some potentially involved in yet to be ...
Qiaoyu Yang +16 more
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BiofilmNeisseria gonorrhoeae, the causative agent of the sexually transmitted infection, gonorrhoea, is known to form biofilms rich in extracellular DNA on human cervical cells. Biofilm formation is conducive to increased antimicrobial resistance and evasion of
Jolyn Pan +4 more
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Characterization of a Baculovirus-Encoded ATP-Dependent DNA Ligase [PDF]
Journal of Virology, 1998 ABSTRACTSequence analysis of theLymantria disparmulticapsid nucleopolyhedrovirus (LdMNPV) genome identified an open reading frame (ORF) encoding a 548-amino-acid (62-kDa) protein that showed 35% amino acid sequence identity with vaccinia virus ATP-dependent DNA ligase. Ligase homologs have not been reported from other baculoviruses.
M N, Pearson, G F, Rohrmann
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The minimal Bacillus subtilis nonhomologous end joining repair machinery. [PDF]
PLoS ONE, 2013 It is widely accepted that repair of double-strand breaks in bacteria that either sporulate or that undergo extended periods of stationary phase relies not only on homologous recombination but also on a minimal nonhomologous end joining (NHEJ) system ...
Miguel de Vega
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Identification of Novel Inhibitors of Escherichia coli DNA Ligase (LigA)
Molecules, 2021 Present in all organisms, DNA ligases catalyse the formation of a phosphodiester bond between a 3′ hydroxyl and a 5′ phosphate, a reaction that is essential for maintaining genome integrity during replication and repair.
Arqam Alomari +10 more
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The Transcriptional Response to DNA-Double-Strand Breaks in Physcomitrella patens. [PDF]
PLoS ONE, 2016 The model bryophyte Physcomitrella patens is unique among plants in supporting the generation of mutant alleles by facile homologous recombination-mediated gene targeting (GT).
Yasuko Kamisugi +2 more
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Use of ATP, dATP and their α-thio derivatives to study DNA ligase adenylation [PDF]
Biochemical Journal, 1990 Bacteriophage-T4 and human type I DNA ligases were found capable of self-adenylating upon exposure to both ribo- and deoxyribo-[alpha-35S]thio-ATP. However, the joining reaction does not take place in the presence of the deoxyribotriphosphates. Enzyme adenylation is reversed in all cases by an excess of PPi, but the rate of reversion is lower with thio
A Montecucco +4 more
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Template-directed ligation of tethered mononucleotides by t4 DNA ligase for kinase ribozyme selection. [PDF]
PLoS ONE, 2010 BACKGROUND: In vitro selection of kinase ribozymes for small molecule metabolites, such as free nucleosides, will require partition systems that discriminate active from inactive RNA species.
David G Nickens +3 more
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Characterization of an ATP-dependent DNA ligase encoded by Chlorella virus PBCV-1 [PDF]
Journal of Virology, 1997 We report that Chlorella virus PBCV-1 encodes a 298-amino-acid ATP-dependent DNA ligase. The PBCV-1 enzyme is the smallest member of the covalent nucleotidyl transferase superfamily, which includes the ATP-dependent polynucleotide ligases and the GTP-dependent RNA capping enzymes.
Ho, C. Kiong +2 more
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PprA contributes to Deinococcus radiodurans resistance to nalidixic acid, genome maintenance after DNA damage and interacts with deinococcal topoisomerases. [PDF]
PLoS ONE, 2014 PprA is known to contribute to Deinococcus radiodurans' remarkable capacity to survive a variety of genotoxic assaults. The molecular bases for PprA's role(s) in the maintenance of the damaged D.
Swathi Kota +4 more
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