Results 41 to 50 of about 2,162,763 (370)
Randomness and optimality in enhanced DNA ligation with crowding effects [PDF]
Phys. Rev. Research 2, 013360 (2020), 2019 Enzymatic ligation is essential for the synthesis of long DNA. However, the number of ligated products exponentially decays as the DNA synthesis proceeds in a random manner. The controlling of ligation randomness is of importance to suppress exponential decay and demonstrate an efficient synthesis of long DNA. Here, we report the analysis of randomness
arxiv +1 more source
DNA Nanotechnology Meets Nanophotonics [PDF]
Nano Lett. 20 8430 (2020), 2021 In the past decades, DNA has been intensely studied and exploited in different research areas of nanoscience and nanotechnology. At first glance, DNA-based nanophotonics seems to deviate quite far from the original goal of Nadrian Seeman, the founder of DNA nanotechnology, who hoped to organize biological entities using DNA in high-resolution crystals.
arxiv +1 more source
Biallelic mutations in DNA ligase 1 underlie a spectrum of immune deficiencies
Journal of Clinical Investigation, 2018 We report the molecular, cellular, and clinical features of 5 patients from 3 kindreds with biallelic mutations in the autosomal LIG1 gene encoding DNA ligase 1.
Patrick Maffucci +18 more
semanticscholar +1 more source
IMG-DNA: Approximate DNA Storage for Images [PDF]
, 2021 Deoxyribonucleic Acid (DNA) as a storage medium with high density and long-term preservation properties can satisfy the requirement of archival storage for rapidly increased digital volume. The read and write processes of DNA storage are error-prone. Images widely used in social media have the properties of fault tolerance which are well fitted to the ...
arxiv +1 more source
Characterization of a temperature-sensitive DNA ligase from Escherichia coli [PDF]
, 2004 DNA ligases are essential enzymes in cells due to their ability to join DNA strand breaks formed during DNA replication. Several temperature-sensitive mutant strains of Escherichia coli, including strain GR501, have been described which can be ...
Bowater, Laura +8 more
core +1 more source
Nucleic Acids Research, 2017 As Cpf1 cleaves double-stranded DNA in a staggered way, it can be used in DNA assembly. However, the Cpf1 cleavage was found to be inaccurate, which may cause errors in DNA assembly.
Chao Lei +5 more
semanticscholar +1 more source
The phage T4 DNA ligase in vivo improves the survival-coupled bacterial mutagenesis
Microbial Cell Factories, 2019 Background Microbial mutagenesis is an important avenue to acquire microbial strains with desirable traits for industry application. However, mutagens either chemical or physical used often leads narrow library pool due to high lethal rate.
Junshu Wang +7 more
doaj +1 more source
Poly(dA:dT)-rich DNAs are highly flexible in the context of DNA looping [PDF]
PLOS ONE 8(10): e75799, 2013 Large-scale DNA deformation is ubiquitous in transcriptional regulation in prokaryotes and eukaryotes alike. Though much is known about how transcription factors and constellations of binding sites dictate where and how gene regulation will occur, less is known about the role played by the intervening DNA. In this work we explore the effect of sequence
arxiv +1 more source
Differential recruitment of DNA Ligase I and III to DNA repair sites [PDF]
, 2006 DNA ligation is an essential step in DNA replication, repair and recombination. Mammalian cells contain three DNA Ligases that are not interchangeable although they use the same catalytic reaction mechanism.
Cardoso, M. Cristina +3 more
core +3 more sources
Human DNA ligase IV is able to use NAD+ as an alternative adenylation donor for DNA ends ligation
Nucleic Acids Research, 2018 All the eukaryotic DNA ligases are known to use adenosine triphosphate (ATP) for DNA ligation. Here, we report that human DNA ligase IV, a key enzyme in DNA double-strand break (DSB) repair, is able to use NAD+ as a substrate for double-stranded DNA ...
Shih-Hsun Chen, Xiaochun Yu
semanticscholar +1 more source