Results 41 to 50 of about 208,982 (285)

Purification and Characterization of DNA Ligase III from Bovine Testes [PDF]

Journal of Biological Chemistry, 1995
Mammalian cell nuclei contain three biochemically distinct DNA ligases. In the present study we have found high levels of DNA ligase I and DNA ligase III activity in bovine testes and have purified DNA ligase III to near homogeneity. The high level of DNA ligase III suggests a role for this enzyme in meiotic recombination.
Zachary B. Mackey, Intisar Husain, William Ramos, Alan E. Tomkinson, William Burkhart, Mary B. Moyer, Jeffrey M. Besterman, Jingwen Chen   +7 more
openaire   +3 more sources

C9orf72 ALS‐causing mutations lead to mislocalization and aggregation of nucleoporin Nup107 into stress granules

FEBS Letters, EarlyView.
Mutations in the C9orf72 gene represent the most common genetic cause of amyotrophic lateral sclerosis (ALS), a fatal neurodegenerative disease. Using patient‐derived neurons and C. elegans models, we find that the nucleoporin Nup107 is dysregulated in C9orf72‐associated ALS. Conversely, reducing Nup107 levels mitigates disease‐related changes.
Saygın Bilican, Yara Nabawi, William Hongyu Zhang, Dunja Petrovic, Markus Wehrmann, Sara Muñoz‐García, Seda Koyuncu, David Vilchez   +7 more
wiley   +1 more source

Four Parallel Pathways in T4 Ligase‐Catalyzed Repair of Nicked DNA with Diverse Bending Angles

Advanced Science
The structural diversity of biological macromolecules in different environments contributes complexity to enzymological processes vital for cellular functions.
Na Li, Jianbing Ma, Hang Fu, Zhiwei Yang, Chunhua Xu, Haihong Li, Yimin Zhao, Yizhen Zhao, Shuyu Chen, Lu Gou, Xinghua Zhang, Shengli Zhang, Ming Li, Ximiao Hou, Lei Zhang, Ying Lu   +15 more
doaj   +1 more source

USP48 restrains resection by site-specific cleavage of the BRCA1 ubiquitin mark from H2A

Nature Communications, 2018
BRCA1 ligase activity is tightly regulated to maintain genome stability and confer DNA double strand repair. Here the authors identify USP48 as a H2A deubiquitinating enzyme that acts as a BRCA1 E3 ligase antagonist and characterize its role during DNA ...
Michael Uckelmann, Ruth M. Densham, Roy Baas, Herrie H. K. Winterwerp, Alexander Fish, Titia K. Sixma, Joanna R. Morris   +6 more
doaj   +1 more source

DNA Ligases: Progress and Prospects [PDF]

Journal of Biological Chemistry, 2009
DNA ligases seal 5'-PO4 and 3'-OH polynucleotide ends via three nucleotidyl transfer steps involving ligase-adenylate and DNA-adenylate intermediates. DNA ligases are essential guardians of genomic integrity, and ligase dysfunction underlies human genetic disease syndromes.
openaire   +3 more sources

Mapping the evolution of mitochondrial complex I through structural variation

FEBS Letters, EarlyView.
Respiratory complex I (CI) is crucial for bioenergetic metabolism in many prokaryotes and eukaryotes. It is composed of a conserved set of core subunits and additional accessory subunits that vary depending on the organism. Here, we categorize CI subunits from available structures to map the evolution of CI across eukaryotes. Respiratory complex I (CI)
Dong‐Woo Shin, Tingting Chen, James A. Letts   +2 more
wiley   +1 more source

Reciprocal control of viral infection and phosphoinositide dynamics

FEBS Letters, EarlyView.
Phosphoinositides, although scarce, regulate key cellular processes, including membrane dynamics and signaling. Viruses exploit these lipids to support their entry, replication, assembly, and egress. The central role of phosphoinositides in infection highlights phosphoinositide metabolism as a promising antiviral target.
Marie Déborah Bancilhon, Bruno Mesmin
wiley   +1 more source

Ligase-mediated construction of branched DNA strands: a novel DNA joining activity catalyzed by T4 DNA ligase [PDF]

Nucleic Acids Research, 2004
Branched nucleic acid strands exist as intermediates in certain biological reactions, and bifurcating DNA also presents interesting opportunities in biotechnological applications. We describe here how T4 DNA ligase can be used for efficient construction of DNA molecules having one 5' end but two distinct 3' ends that extend from the 2' and 3' carbons ...
Maritha Mendel-Hartvig, Ulf Landegren, Anil Kumar   +2 more
openaire   +3 more sources

CCT4 promotes tunneling nanotube formation

FEBS Letters, EarlyView.
Tunneling nanotubes (TNTs) are membranous tunnel‐like structures that transport molecules and organelles between cells. They vary in thickness, and thick nanotubes often contain microtubules in addition to actin fibers. We found that cells expressing monomeric CCT4 generate many thick TNTs with tubulin.
Miyu Enomoto, Akiko Asada, Taro Saito, Kanae Ando   +3 more
wiley   +1 more source

Case Report: Wide Spectrum of Manifestations of Ligase IV Deficiency: Report of 3 Cases

Frontiers in Immunology, 2022
DNA ligase IV deficiency is a rare autosomal recessive disorder associated with impaired DNA repair mechanisms. Most patients with DNA repair defects present with neurologic deficits, combined immunodeficiency, bone marrow failure, and/or hematologic ...
Ana Costa e Castro, Raquel Maia, Sara Batalha, João Parente Freixo, Catarina Martins, Catarina Martins, Conceição Neves, Ana Isabel Cordeiro, João Farela Neves, João Farela Neves, João Farela Neves   +10 more
doaj   +1 more source