Results 41 to 50 of about 2,416,649 (374)

The CCTL (Cpf1-assisted Cutting and Taq DNA ligase-assisted Ligation) method for efficient editing of large DNA constructs in vitro

Nucleic Acids Research, 2017
As Cpf1 cleaves double-stranded DNA in a staggered way, it can be used in DNA assembly. However, the Cpf1 cleavage was found to be inaccurate, which may cause errors in DNA assembly.
Chao Lei, Shiyuan Li, Jia-Kun Liu, Xuan Zheng, Guoping Zhao, Jin Wang   +5 more
semanticscholar   +1 more source

Direct comparison of nick-joining activity of the nucleic acid ligases from bacteriophage T4 [PDF]

, 2006
The genome of bacteriophage T4 encodes three polynucleotide ligases, which seal the backbone of nucleic acids during infection of host bacteria. The T4Dnl (T4 DNA ligase) and two RNA ligases [T4Rnl1 (T4 RNA ligase 1) and T4Rnl2] join a diverse array of ...
Amitsur, Arabshahi, Bebenek, Begley, Bellacosa, Blondal, Blondal, Cherepanov, Cherepanov, Clepet, Della, Desmond R. Bullard, Doherty, Engler, Fareed, Higgins, Ho, Ho, Kaufmann, Keppetipola, Kornberg, Kuhn, Lavesa-Curto, Lehman, Markham, Martins, Martins, Miller, Moore, Nandakumar, Nandakumar, Nandakumar, Nick McElhinny, Omari, Pascal, Pitcher, Pitcher, Richard P. Bowater, Rossi, Ruiz, Runnels, Rydberg, Sambrook, Sekiguchi, Shuman, Shuman, Snopek, Sriskanda, Stuart, Timson, Tomkinson, Wang, Wang, Weiss, Wilkinson, Wilkinson, Wilkinson, Wood, Wood, Worthey, Yin, Yin   +61 more
core   +5 more sources

Biallelic mutations in DNA ligase 1 underlie a spectrum of immune deficiencies

Journal of Clinical Investigation, 2018
We report the molecular, cellular, and clinical features of 5 patients from 3 kindreds with biallelic mutations in the autosomal LIG1 gene encoding DNA ligase 1.
Patrick Maffucci, J. Chávez, Thomas J. Jurkiw, P. O'Brien, J. Abbott, P. Reynolds, A. Worth, L. Notarangelo, K. Felgentreff, P. Cortes, B. Boisson, Lin Radigan, A. Cobat, C. Dinakar, Mohammad S. Ehlayel, T. Ben-Omran, E. Gelfand, J. Casanova, C. Cunningham-Rundles   +18 more
semanticscholar   +1 more source

Analysis of ligation and DNA binding by Escherichia coli DNA ligase (LigA). [PDF]

, 2005
NAD+-dependent DNA ligases are essential enzymes in bacteria, with the most widely studied of this class of enzymes being LigA from Escherichia coli. NAD+-dependent DNA ligases comprise several discrete structural domains, including a BRCT domain at the ...
Adam Wilkinson, Alexandra Bonner, Altschul, Andrew Hemmings, Andrew Smith, Baer, Bork, Brotz-Oesterhelt, Callebaut, Derbyshire, Dermody, Desmond Bullard, Doherty, Doherty, Doherty, Feng, Gajiwala, Georlette, Georlette, Glover, Gong, Grawunder, Guex, Gunther, Halligan, Heather Sayer, Herrmann, Huyton, Jeon, Joo, Kaczmarek, Kodama, Krishnan, Lavesa-Curto, Lee, Legrain, Lehman, Manuel Lavesa-Curto, Masson, Odell, Pascal, Richard Bowater, Sambrook, Shuman, Singleton, Sriskanda, Sriskanda, Sriskanda, Sriskanda, Subramanya, Timson, Timson, Wieland, Wilkinson, Wilkinson, Williams, Yamane, Yamane, Zhang, Zimmerman   +59 more
core   +1 more source

A single-molecule sequencing assay for the comprehensive profiling of T4 DNA ligase fidelity and bias during DNA end-joining

Nucleic Acids Research, 2018
DNA ligases are key enzymes in molecular and synthetic biology that catalyze the joining of breaks in duplex DNA and the end-joining of DNA fragments. Ligation fidelity (discrimination against the ligation of substrates containing mismatched base pairs ...
V. Potapov, J. L. Ong, B. Langhorst, Katharina Bilotti, Daniel H. Cahoon, B. Canton, Thomas F. Knight, T. C. Evans, G. Lohman   +8 more
semanticscholar   +1 more source

NAD+-dependent DNA ligases of Mycobacterium tuberculosis and Streptomyces coelicolor [PDF]

, 2003
Sequencing of the genomes of Mycobacterium tuberculosis H37Rv and Streptomyces coelicolor A3(2) identified putative genes for an NAD+-dependent DNA ligase. We have cloned both open reading frames and overexpressed the protein products in Escherichia coli.
Bowater, Richard, Bullard, Desmond, Day, Jonathan, Kieser, Tobias, Sayer, Heather, Smith, Andrew, Wilkinson, Adam   +6 more
core   +1 more source

Bacteriophage origin of some minimal ATP-dependent DNA ligases: a new structure from Burkholderia pseudomallei with striking similarity to Chlorella virus ligase

Scientific Reports, 2021
DNA ligases, the enzymes responsible for joining breaks in the phosphodiester backbone of DNA during replication and repair, vary considerably in size and structure.
Jolyn Pan, Kjersti Lian, Aili Sarre, Hanna-Kirsti S. Leiros, Adele Williamson   +4 more
doaj   +1 more source

Defective Artemis causes mild telomere dysfunction [PDF]

, 2010
This article has been made available through the Brunel Open Access Publishing Fund.Background: Repair of DNA double strand breaks by non-homologous end joining (NHEJ) requires several proteins including Ku, DNA-PKcs, Artemis, XRCC4, Ligase IV and XLF ...
Slijepcevic, P, Yasaei, H
core   +3 more sources

The phage T4 DNA ligase mediates bacterial chromosome DSBs repair as single component non-homologous end joining

Synthetic and Systems Biotechnology, 2019
DNA double-strand breaks (DSBs) are one of the most lethal forms of DNA damage that is not efficiently repaired in prokaryotes. Certain microorganisms can handle chromosomal DSBs using the error-prone non-homologous end joining (NHEJ) system and ...
Tianyuan Su, Fapeng Liu, Yizhao Chang, Qi Guo, Junshu Wang, Qian Wang, Qingsheng Qi   +6 more
doaj   +1 more source

Ribonucleolytic resection is required for repair of strand displaced nonhomologous end-joining intermediates [PDF]

, 2013
Nonhomologous end-joining (NHEJ) pathways repair DNA double-strand breaks (DSBs) in eukaryotes and many prokaryotes, although it is not reported to operate in the third domain of life, archaea. Here, we describe a complete NHEJ complex, consisting of DNA
Bartlett, Edward, Brissett, Nigel C, Doherty, Aidan J   +2 more
core   +1 more source