Results 51 to 60 of about 2,162,763 (370)
Scientific Reports, 2021 DNA ligases, the enzymes responsible for joining breaks in the phosphodiester backbone of DNA during replication and repair, vary considerably in size and structure.
Jolyn Pan +4 more
doaj +1 more source
Pol I DNA polymerases stimulate DNA end-joining by Escherichia coli DNA ligase [PDF]
, 2018 © 2018 Klenow and Klentaq are the large fragment domains of the Pol I DNA polymerases from Escherichia coli and Thermus aquaticus, respectively. Herein, we show that both polymerases can significantly stimulate complementary intermolecular end-joining ...
LiCata, Vince J., Yang, Yanling
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DNA Ligase 1 is an essential mediator of sister chromatid telomere fusions in G2 cell cycle phase
Nucleic Acids Research, 2018 Fusion of critically short or damaged telomeres is associated with the genomic rearrangements that support malignant transformation. We have demonstrated the fundamental contribution of DNA ligase 4-dependent classical non-homologous end-joining to long ...
K. Liddiard +6 more
semanticscholar +1 more source
ATM mediates oxidative stress-induced dephosphorylation of DNA ligase IIIα [PDF]
, 2006 Among the three mammalian genes encoding DNA ligases, only the LIG3 gene does not have a homolog in lower eukaryotes. In somatic mammalian cells, the nuclear form of DNA ligase IIIα forms a stable complex with the DNA repair protein XRCC1 that is also ...
Alan E. Tomkinson +46 more
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European Journal of Biochemistry, 1992 A novel form of rat liver DNA ligase (molecular mass 100 kDa) can be differentiated from DNA ligase I by several biochemical parameters. It is a more heat‐labile enzyme and unable to join bluntended DNA, even in the presence of poly(ethylene glycol) concentrations which stimulate such joining by DNA ligase I and T4 DNA ligase.
R H Elder +3 more
openaire +4 more sources
Nucleic Acids Research, 2017 Ligation-based nucleic acid detection methods are primarily limited to DNA, since they exhibit poor performance on RNA. This is attributed to reduced end-joining efficiency and/or fidelity of ligases. Interestingly, chlorella virus DNA ligase (PBCV-1 DNA
T. Krzywkowski, M. Nilsson
semanticscholar +1 more source
Targeted disruption of the gene encoding DNA ligase IV leads to lethality in embryonic mice [PDF]
, 1998 DNA ligase IV is the most recently identified member of a family of enzymes joining DNA strand breaks in mammalian cell nuclei [1,2]. The enzyme occurs in a complex with the XRCC4 gene product [3], an interaction mediated via its unique carboxyl terminus
Barnes, Deborah E. +4 more
core +1 more source
The Action of DNA Ligase at Abasic Sites in DNA [PDF]
Journal of Biological Chemistry, 1998 Apurinic/apyrimidinic (AP) sites occur frequently in DNA as a result of spontaneous base loss or following removal of a damaged base by a DNA glycosylase. The action of many AP endonuclease enzymes at abasic sites in DNA leaves a 5'-deoxyribose phosphate (dRP) residue that must be removed during the base excision repair process.
Daniel F. Bogenhagen, Kevin G. Pinz
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Specific recognition of a multiply phosphorylated motif in the DNA repair scaffold XRCC1 by the FHA domain of human PNK. [PDF]
, 2009 Short-patch repair of DNA single-strand breaks and gaps (SSB) is coordinated by XRCC1, a scaffold protein that recruits the DNA polymerase and DNA ligase required for filling and sealing the damaged strand.
Ali, Ammar A. E. +3 more
core +3 more sources
Closing the gap on DNA ligase [PDF]
Structure, 1996 The crystal structure of T7 DNA ligase complexed with ATP illuminates the mechanism of covalent catalysis by a superfamily of nucleotidyl transferases that includes the ATP-dependent polynucleotide ligases and the GTP-dependent mRNA capping enzymes.
openaire +3 more sources