Results 51 to 60 of about 337,826 (349)

Saccharomyces cerevisiae DNA ligase IV supports imprecise end joining independently of its catalytic activity. [PDF]

PLoS Genetics, 2013
DNA ligase IV (Dnl4 in budding yeast) is a specialized ligase used in non-homologous end joining (NHEJ) of DNA double-strand breaks (DSBs). Although point and truncation mutations arise in the human ligase IV syndrome, the roles of Dnl4 in DSB repair ...
Kishore K Chiruvella, Zhuobin Liang, Shanda R Birkeland, Venkatesha Basrur, Thomas E Wilson   +4 more
doaj   +1 more source

Spatial and temporal cellular responses to single-strand breaks in human cells [PDF]

, 2003
DNA single-strand breaks (SSB) are one of the most frequent DNA lesions produced by reactive oxygen species and during DNA metabolism, but the analysis of cellular responses to SSB remains difficult due to the lack of an experimental method to produce ...
Caldecott, Keith W, Lan, Li, Mori, Toshio, Okano, Satoshi, Yasui, Akira   +4 more
core   +4 more sources

Allogeneic hematopoietic stem cell transplantation in two brothers with DNA ligase IV deficiency: a case report and review of the literature

BMC Pediatrics, 2019
Background DNA ligase IV deficiency is a rare autosomal recessive disorder caused by hypomorphic mutations in the DNA ligase IV (LIG4) gene. DNA ligase IV is an essential protein for the development of a healthy immune system as well as for the ...
Sarah Schober, Karin Schilbach, Michaela Doering, Karin M. Cabanillas Stanchi, Ursula Holzer, Patrick Kasteleiner, Jens Schittenhelm, Juergen F. Schaefer, Ingo Mueller, Peter Lang, Rupert Handgretinger   +10 more
doaj   +1 more source

DNA repair: Disorders [PDF]

, 2010
No description ...
Bose, Burg, Caldecott, Chu, Digweed, Fernandez-Capetillo, Fousteri, Fousteri, Friedberg, Gudmundsdottir, Hashimoto, Hoeijmakers, Jiricny, Kraemer, Lavin, Lehmann, Lieber, Ma, Masutani, McCulloch, Meindl, Moldovan, Moshous, Nance, Nouspikel, O'Driscoll, O'Driscoll, O'Driscoll, O'Driscoll, Rass, Stefanini, Stewart, Stracker, Taylor, Vaz, Wu, Zlatanou   +36 more
core   +1 more source

Effects of DNA-binding drugs on T4 DNA ligase [PDF]

Biochemical Journal, 1990
A number of DNA intercalating and externally binding drugs have been found to inhibit nick sealing, cohesive and blunt end ligation, AMP-dependent DNA topoisomerization and EDTA-induced DNA nicking mediated by bacteriophage T4 DNA ligase. The inhibition seems to arise from drug-substrate interaction so that formation of active DNA-Mg2(+)-AMP-enzyme ...
A Montecucco, G PedraliNoy, S Spadari, M Lestingi, G Ciarrocchi   +4 more
openaire   +4 more sources

A stepwise emergence of evolution in the RNA world

FEBS Letters, EarlyView.
How did biological evolution emerge from chemical reactions? This perspective proposes a gradual scenario of self‐organization among RNA molecules, where catalytic feedback on random mixtures plays the central role. Short oligomers cross‐ligate, and self‐assembly enables heritable variations. An event of template‐externalization marks the transition to
Philippe Nghe
wiley   +1 more source

Purification and Characterization of DNA Ligase III from Bovine Testes [PDF]

Journal of Biological Chemistry, 1995
Mammalian cell nuclei contain three biochemically distinct DNA ligases. In the present study we have found high levels of DNA ligase I and DNA ligase III activity in bovine testes and have purified DNA ligase III to near homogeneity. The high level of DNA ligase III suggests a role for this enzyme in meiotic recombination.
Zachary B. Mackey, Intisar Husain, William Ramos, Alan E. Tomkinson, William Burkhart, Mary B. Moyer, Jeffrey M. Besterman, Jingwen Chen   +7 more
openaire   +3 more sources

Insights into pegRNA design from editing of the cardiomyopathy‐associated phospholamban R14del mutation

FEBS Letters, EarlyView.
This study reveals how prime editing guide RNA (pegRNA) secondary structure and reverse transcriptase template length affect prime editing efficiency in correcting the phospholamban R14del cardiomyopathy‐associated mutation. Insights support the design of structurally optimized enhanced pegRNAs for precise gene therapy.
Bing Yao, Qiangbing Yang, Christian J.B. Snijders Blok, Mark A. Daniels, Pieter A. Doevendans, Raymond Schiffelers, Joost P.G. Sluijter, Zhiyong Lei   +7 more
wiley   +1 more source

Four Parallel Pathways in T4 Ligase‐Catalyzed Repair of Nicked DNA with Diverse Bending Angles

Advanced Science
The structural diversity of biological macromolecules in different environments contributes complexity to enzymological processes vital for cellular functions.
Na Li, Jianbing Ma, Hang Fu, Zhiwei Yang, Chunhua Xu, Haihong Li, Yimin Zhao, Yizhen Zhao, Shuyu Chen, Lu Gou, Xinghua Zhang, Shengli Zhang, Ming Li, Ximiao Hou, Lei Zhang, Ying Lu   +15 more
doaj   +1 more source

DNA Ligases: Progress and Prospects [PDF]

Journal of Biological Chemistry, 2009
DNA ligases seal 5'-PO4 and 3'-OH polynucleotide ends via three nucleotidyl transfer steps involving ligase-adenylate and DNA-adenylate intermediates. DNA ligases are essential guardians of genomic integrity, and ligase dysfunction underlies human genetic disease syndromes.
openaire   +3 more sources