Results 61 to 70 of about 2,162,763 (370)

Efficient in situ detection of mRNAs using the Chlorella virus DNA ligase for padlock probe ligation

RNA: A publication of the RNA Society, 2017
Padlock probes are single-stranded DNA molecules that are circularized upon hybridization to their target sequence by a DNA ligase. In the following, the circulated padlock probes are amplified and detected with fluorescently labeled probes complementary
Nils Schneider, Matthias Meier
semanticscholar   +1 more source

The Inhibitory Effect of Non-Substrate and Substrate DNA on the Ligation and Self-Adenylylation Reactions Catalyzed by T4 DNA Ligase. [PDF]

PLoS ONE, 2016
DNA ligases are essential both to in vivo replication, repair and recombination processes, and in vitro molecular biology protocols. Prior characterization of DNA ligases through gel shift assays has shown the presence of a nick site to be essential for ...
Robert J Bauer, Thomas C Evans, Gregory J S Lohman   +2 more
doaj   +1 more source

Direct comparison of nick-joining activity of the nucleic acid ligases from bacteriophage T4 [PDF]

, 2006
The genome of bacteriophage T4 encodes three polynucleotide ligases, which seal the backbone of nucleic acids during infection of host bacteria. The T4Dnl (T4 DNA ligase) and two RNA ligases [T4Rnl1 (T4 RNA ligase 1) and T4Rnl2] join a diverse array of ...
Amitsur, Arabshahi, Bebenek, Begley, Bellacosa, Blondal, Blondal, Cherepanov, Cherepanov, Clepet, Della, Desmond R. Bullard, Doherty, Engler, Fareed, Higgins, Ho, Ho, Kaufmann, Keppetipola, Kornberg, Kuhn, Lavesa-Curto, Lehman, Markham, Martins, Martins, Miller, Moore, Nandakumar, Nandakumar, Nandakumar, Nick McElhinny, Omari, Pascal, Pitcher, Pitcher, Richard P. Bowater, Rossi, Ruiz, Runnels, Rydberg, Sambrook, Sekiguchi, Shuman, Shuman, Snopek, Sriskanda, Stuart, Timson, Tomkinson, Wang, Wang, Weiss, Wilkinson, Wilkinson, Wilkinson, Wood, Wood, Worthey, Yin, Yin   +61 more
core   +4 more sources

Mammalian DNA ligases. Biosynthesis and intracellular localization of DNA ligase I.

Journal of Biological Chemistry, 1990
Mammalian DNA ligase I is presumed to act in DNA replication. Rabbit antibodies against the homogeneous enzyme from calf thymus inhibited DNA ligase I activity and consistently recognized a single polypeptide of 125 kDa when cells from an established bovine kidney cell line (MDBK) were lysed rapidly by a variety of procedures and subjected to ...
Alan E. Tomkinson, Tomas Lindahl, Dana D. Lasko   +2 more
openaire   +3 more sources

A high-throughput assay for the comprehensive profiling of DNA ligase fidelity

Nucleic Acids Research, 2015
DNA ligases have broad application in molecular biology, from traditional cloning methods to modern synthetic biology and molecular diagnostics protocols.
G. Lohman, R. Bauer, N. Nichols, Laurie Mazzola, Joanna Bybee, Danielle Rivizzigno, Elizabeth Cantin, T. C. Evans   +7 more
semanticscholar   +1 more source

Saccharomyces cerevisiae DNA ligase IV supports imprecise end joining independently of its catalytic activity. [PDF]

PLoS Genetics, 2013
DNA ligase IV (Dnl4 in budding yeast) is a specialized ligase used in non-homologous end joining (NHEJ) of DNA double-strand breaks (DSBs). Although point and truncation mutations arise in the human ligase IV syndrome, the roles of Dnl4 in DSB repair ...
Kishore K Chiruvella, Zhuobin Liang, Shanda R Birkeland, Venkatesha Basrur, Thomas E Wilson   +4 more
doaj   +1 more source

Alternative Okazaki Fragment Ligation Pathway by DNA Ligase III

Genes, 2015
Higher eukaryotes have three types of DNA ligases: DNA ligase 1 (Lig1), DNA ligase 3 (Lig3) and DNA ligase 4 (Lig4). While Lig1 and Lig4 are present in all eukaryotes from yeast to human, Lig3 appears sporadically in evolution and is uniformly present ...
H. Arakawa, G. Iliakis
semanticscholar   +1 more source

Stealing the spotlight: CUL4-DDB1 ubiquitin ligase docks WD40-repeat proteins to destroy

Cell Division, 2007
Recent investigation of Cullin 4 (CUL4) has ushered this class of multiprotein ubiquitin E3 ligases to center stage as critical regulators of diverse processes including cell cycle regulation, developmental patterning, DNA replication, DNA damage and ...
Zhang Hui, Higa Leigh
doaj   +1 more source

Allogeneic hematopoietic stem cell transplantation in two brothers with DNA ligase IV deficiency: a case report and review of the literature

BMC Pediatrics, 2019
Background DNA ligase IV deficiency is a rare autosomal recessive disorder caused by hypomorphic mutations in the DNA ligase IV (LIG4) gene. DNA ligase IV is an essential protein for the development of a healthy immune system as well as for the ...
Sarah Schober, Karin Schilbach, Michaela Doering, Karin M. Cabanillas Stanchi, Ursula Holzer, Patrick Kasteleiner, Jens Schittenhelm, Juergen F. Schaefer, Ingo Mueller, Peter Lang, Rupert Handgretinger   +10 more
doaj   +1 more source

Effects of DNA-binding drugs on T4 DNA ligase [PDF]

Biochemical Journal, 1990
A number of DNA intercalating and externally binding drugs have been found to inhibit nick sealing, cohesive and blunt end ligation, AMP-dependent DNA topoisomerization and EDTA-induced DNA nicking mediated by bacteriophage T4 DNA ligase. The inhibition seems to arise from drug-substrate interaction so that formation of active DNA-Mg2(+)-AMP-enzyme ...
A Montecucco, G PedraliNoy, S Spadari, M Lestingi, G Ciarrocchi   +4 more
openaire   +4 more sources