Results 81 to 90 of about 2,162,763 (370)

USP48 restrains resection by site-specific cleavage of the BRCA1 ubiquitin mark from H2A

Nature Communications, 2018
BRCA1 ligase activity is tightly regulated to maintain genome stability and confer DNA double strand repair. Here the authors identify USP48 as a H2A deubiquitinating enzyme that acts as a BRCA1 E3 ligase antagonist and characterize its role during DNA ...
Michael Uckelmann, Ruth M. Densham, Roy Baas, Herrie H. K. Winterwerp, Alexander Fish, Titia K. Sixma, Joanna R. Morris   +6 more
doaj   +1 more source

Defects Can Increase the Melting Temperature of DNA-Nanoparticle Assemblies [PDF]

J. Phys. Chem. B, 110 (2006) 16393-16396, 2007
DNA-gold nanoparticle assemblies have shown promise as an alternative technology to DNA microarrays for DNA detection and RNA profiling. Understanding the effect of DNA sequences on the melting temperature of the system is central to developing reliable detection technology.
arxiv   +1 more source

Ligase-mediated construction of branched DNA strands: a novel DNA joining activity catalyzed by T4 DNA ligase [PDF]

Nucleic Acids Research, 2004
Branched nucleic acid strands exist as intermediates in certain biological reactions, and bifurcating DNA also presents interesting opportunities in biotechnological applications. We describe here how T4 DNA ligase can be used for efficient construction of DNA molecules having one 5' end but two distinct 3' ends that extend from the 2' and 3' carbons ...
Maritha Mendel-Hartvig, Ulf Landegren, Anil Kumar   +2 more
openaire   +3 more sources

Efficient DNA ligation in DNA–RNA hybrid helices by Chlorella virus DNA ligase

Nucleic Acids Research, 2013
Single-stranded DNA molecules (ssDNA) annealed to an RNA splint are notoriously poor substrates for DNA ligases. Herein we report the unexpectedly efficient ligation of RNA-splinted DNA by Chlorella virus DNA ligase (PBCV-1 DNA ligase). PBCV-1 DNA ligase
G. Lohman, Yinhua Zhang, A. Zhelkovsky, E. Cantor, T. C. Evans   +4 more
semanticscholar   +1 more source

Targeting the MDM2‐MDM4 interaction interface reveals an otherwise therapeutically active wild‐type p53 in colorectal cancer

Molecular Oncology, EarlyView.
This study investigates an alternative approach to reactivating the oncosuppressor p53 in cancer. A short peptide targeting the association of the two p53 inhibitors, MDM2 and MDM4, induces an otherwise therapeutically active p53 with unique features that promote cell death and potentially reduce toxicity towards proliferating nontumor cells.
Sonia Valentini, Giada Mele, Marika Attili, Maria Rita Assenza, Fulvio Saccoccia, Francesca Sardina, Cinzia Rinaldo, Roberto Massari, Nicola Tirelli, Alfredo Pontecorvi, Fabiola Moretti   +10 more
wiley   +1 more source

Defective Artemis causes mild telomere dysfunction [PDF]

, 2010
This article has been made available through the Brunel Open Access Publishing Fund.Background: Repair of DNA double strand breaks by non-homologous end joining (NHEJ) requires several proteins including Ku, DNA-PKcs, Artemis, XRCC4, Ligase IV and XLF ...
Slijepcevic, P, Yasaei, H
core   +3 more sources

Mitochondrial DNA Ligase Is Dispensable for the Viability of Cultured Cells but Essential for mtDNA Maintenance*

Journal of Biological Chemistry, 2013
Background: Nonviability of cells lacking mitochondrial DNA ligase suggests essential function of this enzyme. Results: We report the isolation of viable Lig3−/− cells, which lack mtDNA.
I. Shokolenko, Rafik Z. Fayzulin, S. Katyal, P. Mckinnon, G. Wilson, M. Alexeyev   +5 more
semanticscholar   +1 more source

ShcD adaptor protein drives invasion of triple negative breast cancer cells by aberrant activation of EGFR signaling

Molecular Oncology, EarlyView.
We identified adaptor protein ShcD as upregulated in triple‐negative breast cancer and found its expression to be correlated with reduced patient survival and increased invasion in cell models. Using a proteomic screen, we identified novel ShcD binding partners involved in EGFR signaling pathways.
Hayley R. Lau, Hayley S. Smith, Begüm Alural, Claire E. Martin, Laura A. New, Manali Tilak, Sara L. Banerjee, Hannah N. Robeson, Nicolas Bisson, Anne‐Claude Gingras, Jasmin Lalonde, Nina Jones   +11 more
wiley   +1 more source

Case Report: Wide Spectrum of Manifestations of Ligase IV Deficiency: Report of 3 Cases

Frontiers in Immunology, 2022
DNA ligase IV deficiency is a rare autosomal recessive disorder associated with impaired DNA repair mechanisms. Most patients with DNA repair defects present with neurologic deficits, combined immunodeficiency, bone marrow failure, and/or hematologic ...
Ana Costa e Castro, Raquel Maia, Sara Batalha, João Parente Freixo, Catarina Martins, Catarina Martins, Conceição Neves, Ana Isabel Cordeiro, João Farela Neves, João Farela Neves, João Farela Neves   +10 more
doaj   +1 more source

Making ends meet: repairing breaks in bacterial DNA by non-homologous end-joining. [PDF]

, 2006
DNA double-strand breaks (DSBs) are one of the most dangerous forms of DNA lesion that can result in genomic instability and cell death. Therefore cells have developed elaborate DSB-repair pathways to maintain the integrity of genomic DNA.
Bowater, Richard, Doherty, Aidan J
core   +5 more sources