Results 221 to 230 of about 145,020 (273)

High‐Throughput Engineering and Modification of Non‐Ribosomal Peptide Synthetases Based on Golden Gate Assembly

Angewandte Chemie, EarlyView.
A Golden Gate Assembly (GGA)‐based method was developed for the efficient assembly of natural and engineered non‐ribosomal peptide synthetases (NRPS). This method has enabled the creation of NRPS libraries to generate novel peptides in high‐throughput as well as the targeted derivatisation of natural products (NPs).
Adrian Podolski, Timon A. Lindeboom, Leonard Präve, Maryam Dehghan, Hannah A. Minas, Janik Kranz, Daniel Schindler, Helge B. Bode   +7 more
wiley   +2 more sources

Large scale identification of pellicle and cell-free liquid phase associated proteins in <i>Bacillus amyloliquefaciens</i> L-17. [PDF]

Curr Res Microb Sci
Ouidir T, Hardouin J, Marcato-Romain CE, Girbal-Neuhauser E, Nait Chabane Y.   +4 more
europepmc   +1 more source

Construction of pathogenic Sec16a mutation mouse model using CRISPR/Cas9

Animal Models and Experimental Medicine, EarlyView.
Yaqiang Hu et al. engineered a pathogenic Sec16a mutant mouse model using CRISPR/Cas9 technology. They observed that the Sec16a mutant mice displayed diminished learning and memory capabilities, along with a limb‐clasping phenotype upon tail suspension.
Yaqiang Hu, Zhiyang Zeng, Xinyu Ming, Shuming Yin, Yuting Guan, Liangcai Gao, Dali Li   +6 more
wiley   +1 more source

Precision tuning of ABA signaling by ubiquitination of ABA receptors: modulating protein activity and localization. [PDF]

J Exp Bot
Du C, Zhang Z.
europepmc   +1 more source

Nucleic Acid‐Based Molecular Machines for Biological Applications

Advanced NanoBiomed Research, EarlyView.
Molecular machines are devices assembled from molecules with specific functions. In this review, the development of DNA nanostructures, which are then assembled into molecular machines, is summarized. Their classification and biological applications, such as biosensing, targeted therapy, and molecular circuits are introduced.
Yirong Guo, Xiaolei Zuo, Fangfei Yin
wiley   +1 more source

Biomedical Applications of Nanozymes: An Enzymology Perspective

Angewandte Chemie, EarlyView.
With a lens of enzymology, this review compares nanozymes with enzymes using the Michaelis‐Menten model and differences in KM and kcat along with catalysis in complex biological environment. The implications of these differences in biomedical applications of nanozymes are then described with a statistical analysis of the trend of therapeutic use of ...
Vasily G. Panferov, Xiaohan Zhang, Ka‐Ying Wong, Jung Heon Lee, Juewen Liu   +4 more
wiley   +2 more sources

Alzheimer's Disease Pathogenic Mechanisms: Linking Redox Homeostasis and Mitochondria-Associated Metabolic Pathways Through Nuclear Factor Erythroid 2-Related Factor 2 (Nrf2). [PDF]

Antioxidants (Basel)
Rostagno A, Ghiso J.
europepmc   +1 more source

Phosphoproteomic profiling of feline mammary carcinoma: Insights into tumor grading and potential therapeutic targets. [PDF]

PLoS One
Aruvornlop P, Ploypetch S, Sakcamduang W, Sirivisoot S, Kasantikul T, Roytrakul S, Phaonakrop N, Arya N.   +7 more
europepmc   +1 more source

Reduced Pressure Ionization Enhances Native Mass Spectrometry of Proteins and Protein Complexes

Angewandte Chemie, EarlyView.
Reduced pressure for electrospray ionization of native proteins narrows peak widths and increases signal. This straightforward add‐on to existing native MS platforms improves performance in challenging buffers and enables detection of protein interactions at concentrations as low as 50 nM, without significantly disrupting structure as confirmed by ...
Jake P. Violi, Chong Zhang, William A. Donald   +2 more
wiley   +1 more source

Ubiquitination in macrophage plasticity: from inflammatory to immunometabolic pathways. [PDF]

Front Immunol
Liu J, Paolino M.
europepmc   +1 more source