Results 221 to 230 of about 113,019 (248)
Characterization of an ATP-dependent type I DNA ligase from Arabidopsis thaliana
Plant Molecular Biology, 2001 Here we report the purification and biochemical characterization of recombinant Arabidopsis thaliana DNA ligase I. We show that this ligase requires ATP as a source for adenylation. The calculated Km [ATP] for ligation is 3 microM. This enzyme is able to ligate nicks in oligo(dT)/poly(dA) and oligo(rA)/poly(dT) substrates, but not in oligo(dT)/poly(rA)
You‐Qiang Wu +2 more
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Functional domains of an ATP-dependent DNA ligase 1 1Edited by A. R. Fersht
Journal of Molecular Biology, 1999 Aidan J. Doherty, Dale B. Wigley
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Free-labelled fluorescent method for ATP detection assisted by T4 DNA ligase
Analytical Methods, 2017 A facile, highly selective biosensor was used for ATP quantitative analysis.
Hongyun Huang +5 more
semanticscholar +3 more sources
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The α-thio and/or β-γ-hypophosphate analogs of ATP as cofactors of T4 DNA ligase.
Bioorganic Chemistry, 2016T4 DNA ligase is one of the most commonly used enzymes for in vitro molecular research and a useful model for testing the ligation mechanism of ATP-dependent DNA ligation. To better understand the influence of phosphate group modifications in the ligation process, a series of ATP analogs were tested as cofactors.
Róża Pawłowska +4 more
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Mechanistic and kinetic study of the ATP-dependent DNA ligase of Neisseria meningitidis.
Biochemistry, 2004The gene from Neisseria meningitidis serogroup A, encoding a putative, secreted ATP-dependent DNA ligase was cloned and overexpressed, and the soluble protein was purified. Mass spectrometry indicated that the homogeneous protein was adenylated as isolated, and sedimentation velocity experiments suggested that the enzyme exists as a monomer in solution.
S. Magnet, J. Blanchard
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Bioinformatic analysis of Tetrahymena thermophila ATP dependent DNA ligases
New Biotechnology, 2009 …
H.Açelya Akdamar +2 more
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International Journal of Biological Macromolecules, 2023 The genome sequence of hyperthermophilic archaeon Pyrobaculum calidifontis contains an open reading frame, Pcal_0039, which encodes a putative DNA ligase. Structural analysis disclosed the presence of signature sequences of ATP-dependent DNA ligases. We have heterologously expressed Pcal_0039 gene in Escherichia coli.
Qamar Abbas +4 more
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A sensitive ligase-based ATP electrochemical assay using molecular beacon-like DNA
Biosensors and Bioelectronics, 2010 A sensitive and selective ligase-based signal-on electrochemical sensing method for adenosine-5'-triphosphate (ATP) detection had been developed using molecular beacon (MB)-like DNA. In this method, the biotin-tagged MB-like DNA was self-assembled onto a gold electrode to form a stem-loop structure by means of facile gold-thiol chemistry, which ...
Yonghong Wang +3 more
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mycobacterial DNA ligase D complexed with ATP and MES
, 2018 Stewart Shuman +2 more
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Biochemistry and Cell Biology, 2010
A multiprotein DNA processing complex isolated from Deinococcus radiodurans contains the DNA repair protein PprA, an ATP-type DNA repair ligase (LigB) encoded by the drB0100 gene, and protein kinase activity. An ATP-dependent DNA end-joining activity was detected in the complex.
Swathi Kota +3 more
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A multiprotein DNA processing complex isolated from Deinococcus radiodurans contains the DNA repair protein PprA, an ATP-type DNA repair ligase (LigB) encoded by the drB0100 gene, and protein kinase activity. An ATP-dependent DNA end-joining activity was detected in the complex.
Swathi Kota +3 more
semanticscholar +3 more sources