Results 251 to 260 of about 145,020 (273)
Characterization of an ATP-dependent type I DNA ligase from Arabidopsis thaliana. [PDF]
Plant Molecular Biology, 2001 Here we report the purification and biochemical characterization of recombinant Arabidopsis thaliana DNA ligase I. We show that this ligase requires ATP as a source for adenylation. The calculated Km [ATP] for ligation is 3 microM. This enzyme is able to ligate nicks in oligo(dT)/poly(dA) and oligo(rA)/poly(dT) substrates, but not in oligo(dT)/poly(rA)
You‐Qiang Wu +2 more
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Biochemical Pharmacology, 2008 T4 DNA ligase and the ubiquitin activating enzyme (E1), catalyze the synthesis of ATP beta,gamma-bisphosphonate derivatives. Concerning T4 DNA ligase: (i) etidronate (pC(OH)(CH(3))p) displaced the AMP moiety of the complex E-AMP in a concentration dependent manner; (ii) the K(m) values and the rate of synthesis k(cat) (s(-1)), determined for the ...
Marı́a A. Günther Sillero +3 more
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International Journal of Biological Macromolecules, 2023 The genome sequence of hyperthermophilic archaeon Pyrobaculum calidifontis contains an open reading frame, Pcal_0039, which encodes a putative DNA ligase. Structural analysis disclosed the presence of signature sequences of ATP-dependent DNA ligases. We have heterologously expressed Pcal_0039 gene in Escherichia coli.
Qamar Abbas +4 more
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Mechanistic and Kinetic Study of the ATP-Dependent DNA Ligase of Neisseria meningitidis
Biochemistry, 2003 The gene from Neisseria meningitidis serogroup A, encoding a putative, secreted ATP-dependent DNA ligase was cloned and overexpressed, and the soluble protein was purified. Mass spectrometry indicated that the homogeneous protein was adenylated as isolated, and sedimentation velocity experiments suggested that the enzyme exists as a monomer in solution.
Sophie Magnet, John S. Blanchard
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Functional domains of an ATP-dependent DNA ligase 1 1Edited by A. R. Fersht
Journal of Molecular Biology, 1999 Aidan J. Doherty, Dale B. Wigley
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Free-labelled fluorescent method for ATP detection assisted by T4 DNA ligase
Analytical Methods, 2017 A facile, highly selective biosensor was used for ATP quantitative analysis.
Hongyun Huang +5 more
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The α-thio and/or β-γ-hypophosphate analogs of ATP as cofactors of T4 DNA ligase
Bioorganic Chemistry, 2016 T4 DNA ligase is one of the most commonly used enzymes for in vitro molecular research and a useful model for testing the ligation mechanism of ATP-dependent DNA ligation. To better understand the influence of phosphate group modifications in the ligation process, a series of ATP analogs were tested as cofactors.
Róża Pawłowska +4 more
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ATP‐ and NAD+‐dependent DNA ligases share an essential function in the halophilic archaeon Haloferax volcanii [PDF]
Molecular Microbiology, 2005 SummaryDNA ligases join the ends of DNA molecules during replication, repair and recombination. ATP‐dependent ligases are found predominantly in the eukarya and archaea whereas NAD+‐dependent DNA ligases are found only in the eubacteria and in entomopoxviruses.
An Zhao +2 more
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A sensitive ligase-based ATP electrochemical assay using molecular beacon-like DNA
Biosensors and Bioelectronics, 2010A sensitive and selective ligase-based signal-on electrochemical sensing method for adenosine-5'-triphosphate (ATP) detection had been developed using molecular beacon (MB)-like DNA. In this method, the biotin-tagged MB-like DNA was self-assembled onto a gold electrode to form a stem-loop structure by means of facile gold-thiol chemistry, which ...
Xiaoqi Ni +3 more
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MedChemComm, 2012
Identification of compounds distinguishing between NAD+ and ATP-dependent ligases is a key factor to discover new antimicrobials. Based on virtual screening experiments a series of hydroxamates distinguishing between the above two classes of enzymes are synthesized.
Vandna Kukshal +8 more
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Identification of compounds distinguishing between NAD+ and ATP-dependent ligases is a key factor to discover new antimicrobials. Based on virtual screening experiments a series of hydroxamates distinguishing between the above two classes of enzymes are synthesized.
Vandna Kukshal +8 more
openaire +2 more sources