Results 261 to 270 of about 145,020 (273)

A new group of plant-specific ATP-dependent DNA ligases identified by protein phylogeny, hydrophobic cluster analysis and 3-dimensional modelling

Functional Plant Biology, 2005
The eukaryotic ATP-dependent DNA ligases comprise a group of orthologous proteins that have distinct roles in DNA metabolism. In contrast with the well-known DNA ligases of animal cells, the DNA ligases of plant cells are poorly described. Until now, only two DNA ligases (I and IV) genes of Arabidopsis thaliana (L.) Heynh were isolated and ...
Martin Brendel, Martin Brendel, Diego Bonatto, João Antonio Pêgas Henriques, João Antonio Pêgas Henriques   +4 more
openaire   +3 more sources

A functional and structural study of three bacterial nucleic acid-interacting proteins. The story of a Ferric Uptake Regulator, an Oligoribonuclease and an ATP-dependent DNA ligase

, 2020
Nucleic acid-interacting proteins are essential players in cellular processes of all living organisms. In the present study, we explore three psychrophilic-derived proteins involved in gene regulation, RNA degradation and DNA ligation in bacterial cells, with one common feature; they perform their activity by interacting with nucleic acids.
Kristel Berg
openalex   +2 more sources

Interaction of β-L-Adenosine-5′-triphosphate (L-ATP) with Human Deoxycytidine Kinase, Human DNA Primase and T4 DNA Ligase: Does the Chance Direct Enzymatic Enantioselectivity?

Nucleosides and Nucleotides, 1999
We demonstrate that L-ATP: 1) as well as its natural D-enantiomer, acts as a phosphate donor in the reaction catalysed by human deoxycytidine kinase; 2) inhibits human DNA-primase and the ATP-dependent T4 DNA ligase. Thus, the lack of enantioselectivity of the enzymes is more frequent than it was believed a few years ago and we suggest that it would ...
A. Montecucco, Gilles Gosselin, Federico Focher, Annalisa Verri, Jean Louis Imbach, Silvio Spadari, V. Boudou   +6 more
openaire   +3 more sources

ATP-type DNA ligase requires other proteins for its activity in vitro and its operon components for radiation resistance in Deinococcus radiodurans in vivo

Biochemistry and Cell Biology, 2010
A multiprotein DNA processing complex isolated from Deinococcus radiodurans contains the DNA repair protein PprA, an ATP-type DNA repair ligase (LigB) encoded by the drB0100 gene, and protein kinase activity. An ATP-dependent DNA end-joining activity was detected in the complex.
Yogendra S. Rajpurohit, Swathi Kota, Hari S. Misra, Vidya A. Kamble   +3 more
openaire   +3 more sources

Functional domains of an ATP-dependent DNA ligase.

Journal of molecular biology, 1999
The crystal structure of an ATP-dependent DNA ligase from bacteriophage T7 revealed that the protein comprised two structural domains. In order to investigate the biochemical activities of these domains, we have overexpressed them separately and purified them to homogeneity. The larger N-terminal domain retains adenylation and ligase activities, though
A J, Doherty, D B, Wigley
openaire   +1 more source

Interaction of beta-L-adenosine-5 '-triphosphate (L-ATP) with human deoxycytidine kinase, human DNA primase and T4 DNA ligase: Does the chance direct enzymatic enantioselectivity?

1999
We demonstrate that L-ATP: 1) as well as its natural D-enantiomer, acts as a phosphate donor in the reaction catalysed by human deoxycytidine kinase; 2) inhibits human DNA-primase and the ATP-dependent T4 DNA ligase. Thus, the lack of enantioselectivity of the enzymes is more frequent than it was believed a few years ago and we suggest that it would ...
Verri A, Montecucco A, Gosselin G, Boudou V, Spadari S, Focher F   +5 more
openaire   +1 more source

ATP-DEPENDENT DNA LIGASE FROM BACTERIOPHAGE T7 COMPLEX WITH ATP

, 1998
H. S. Subramanya, Aidan J. Doherty, Stephen R. Ashford, Dale B. Wigley   +3 more
openalex   +1 more source

Probing and Enhancing DNA Ligase Specificity via Se‐Atom‐Mutagenesis of ATP for Accurate Gene Synthesis and Detection

Dejin Xu, Bei Hu, Xiaoling Ding, Qin Tong, Ting Li, Jiaxin Li, Danyan Luo, Lu Chen, Yunfan Xu, Jun Zhang, Han Chang Kang, Yang Zhang, Zhen Huang   +12 more
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ATP-DEPENDENT DNA LIGASE FROM PYROCOCCUS FURIOSUS

, 2006
H Nishida, Yoshizumi Ishino, Kosuke Morikawa   +2 more
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ATP/ADP結合型RecAによるE.coli/T4DNA LigaseのDNA末端結合活性促進の生化学的特性

, 2016
此村直人, 新井直人, 古久保哲朗, 柴田武彦   +3 more
openalex   +1 more source