Results 51 to 60 of about 113,019 (248)

A cost-effective method for Illumina small RNA-Seq library preparation using T4 RNA ligase 1 adenylated adapters

Plant Methods, 2012
Background Deep sequencing is a powerful tool for novel small RNA discovery. Illumina small RNA sequencing library preparation requires a pre-adenylated 3’ end adapter containing a 5’,5’-adenyl pyrophosphoryl moiety.
Chen Yun-Ru, Zheng Yi, Liu Bao, Zhong Silin, Giovannoni Jim, Fei Zhangjun   +5 more
doaj   +1 more source

Baicalein resensitizes tamoxifen‐resistant breast cancer cells by reducing aerobic glycolysis and reversing mitochondrial dysfunction via inhibition of hypoxia‐inducible factor‐1α

Clinical and Translational Medicine, 2021
Drug resistance is a major hurdle for the effectiveness of tamoxifen (TAM) to provide clinical benefit. Therefore, it is essential to identify a sensitizer that could be used to improve TAM efficacy in treating TAM‐resistant breast cancer.
Yan Chen, Jingyu Zhang, Minqin Zhang, Yuxuan Song, Yue Zhang, Shuangqin Fan, Shuang Ren, Lingyun Fu, Nenling Zhang, Hui Hui, Xiangchun Shen   +10 more
doaj   +1 more source

Ataxia-telangiectasia mutated interacts with Parkin and induces mitophagy independent of kinase activity. Evidence from mantle cell lymphoma

Haematologica, 2020
Ataxia telangiectasia mutated (ATM), a critical DNA damage sensor with protein kinase activity,is frequently altered in human cancers including mantle cell lymphoma (MCL).
Aloke Sarkar, Christine M. Stellrecht, Hima V. Vangapandu, Mary Ayres, Benny A. Kaipparettu, Jun Hyoung Park, Kumudha Balakrishnan, Jared K. Burks, Tej K. Pandita, Walter N. Hittelman, Sattva S. Neelapu, Varsha Gandhi   +11 more
doaj   +1 more source

DNA methylation modifier LSH inhibits p53 ubiquitination and transactivates p53 to promote lipid metabolism

Epigenetics & Chromatin, 2019
Background The stability of p53 is mainly controlled by ubiquitin-dependent degradation, which is triggered by the E3 ubiquitin ligase MDM2. The chromatin modifier lymphoid-specific helicase (LSH) is essential for DNA methylation and cancer progression ...
Ling Chen, Ying Shi, Na Liu, Zuli Wang, Rui Yang, Bin Yan, Xiaoli Liu, Weiwei Lai, Yating Liu, Desheng Xiao, Hu Zhou, Yan Cheng, Ya Cao, Shuang Liu, Zanxian Xia, Yongguang Tao   +15 more
doaj   +1 more source

Reciprocal control of viral infection and phosphoinositide dynamics

FEBS Letters, EarlyView.
Phosphoinositides, although scarce, regulate key cellular processes, including membrane dynamics and signaling. Viruses exploit these lipids to support their entry, replication, assembly, and egress. The central role of phosphoinositides in infection highlights phosphoinositide metabolism as a promising antiviral target.
Marie Déborah Bancilhon, Bruno Mesmin
wiley   +1 more source

Ginsenoside Re Treatment Attenuates Myocardial Hypoxia/Reoxygenation Injury by Inhibiting HIF-1α Ubiquitination

Frontiers in Pharmacology, 2020
Previous studies have shown an attenuating effect of ginsenoside Re on myocardial injury induced by hypoxia/reoxygenation (H/R). However, the underlying mechanism remains unclear.
Huiyuan Sun, Huiyuan Sun, Huiyuan Sun, Shukuan Ling, Dingsheng Zhao, Jianwei Li, Yang Li, Hua Qu, Ruikai Du, Ying Zhang, Feng Xu, Yuheng Li, Caizhi Liu, Guohui Zhong, Shuai Liang, Zizhong Liu, Xingcheng Gao, Xiaoyan Jin, Yingxian Li, Dazhuo Shi   +19 more
doaj   +1 more source

Crosstalk between the ribosome quality control‐associated E3 ubiquitin ligases LTN1 and RNF10

FEBS Letters, EarlyView.
Loss of the E3 ligase LTN1, the ubiquitin‐like modifier UFM1, or the deubiquitinating enzyme UFSP2 disrupts endoplasmic reticulum–ribosome quality control (ER‐RQC), a pathway that removes stalled ribosomes and faulty proteins. This disruption may trigger a compensatory response to ER‐RQC defects, including increased expression of the E3 ligase RNF10 ...
Yuxi Huang, Satoshi Hashimoto, Sota Ito, Chisato Kikuguchi, Miho Hoshi, Kiyoshi Yamaguchi, Yoichi Furukawa, Toru Suzuki, Toshifumi Inada   +8 more
wiley   +1 more source

R2D ligase: Unveiling a novel DNA ligase with surprising DNA‐to‐RNA ligation activity

Biotechnology Journal
DNA ligases catalyze bond formation in the backbone of nucleic acids via the formation of a phosphodiester bond between adjacent 5′ phosphates and 3′ hydroxyl groups on one strand of the duplex.
S. Gundesø, U. Rothweiler, Elise Heimland, Yvonne Piotrowski, Inger Kristine Rødum, Jenny Johansson Söderberg, Ildikó Moustaka Gábor, Terese Solstad, Adele Williamson, O. Lanes, Bernd Ketelsen Striberny   +10 more
semanticscholar   +1 more source

Disordered but rhythmic—the role of intrinsic protein disorder in eukaryotic circadian timing

FEBS Letters, EarlyView.
Unstructured domains known as intrinsically disordered regions (IDRs) are present in nearly every part of the eukaryotic core circadian oscillator. IDRs enable many diverse inter‐ and intramolecular interactions that support clock function. IDR conformations are highly tunable by post‐translational modifications and environmental conditions, which ...
Emery T. Usher, Jacqueline F. Pelham
wiley   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source