Results 91 to 100 of about 2,144,804 (323)
Structure and Function of a Mycobacterial NHEJ DNA Repair Polymerase [PDF]
, 2006 Non homologous end-joining (NHEJ)-mediated repair of DNA double-strand breaks in prokaryotes requires Ku and a specific multidomain DNA ligase (LigD).
Aidan J. Doherty +59 more
core +1 more source
SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
FEBS Open Bio, EarlyView.The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source
New Aspects of HECT-E3 Ligases in Cell Senescence and Cell Death of Plants
Plants, 2019 Plant cells undergo massive orderly changes in structure, biochemistry, and gene expression during cell senescence. These changes cannot be distinguished from the hydrolysis/degradation function controlled by the ubiquitination pathway, autophagy, and ...
Wei Lan, Ying Miao
doaj +1 more source
Role of the ubiquitin-selective CDC-48/UFD-1/NPL-4 chaperone in DNA replication [PDF]
, 2012 Faithful transmission of genomic information requires tight spatiotemporal regulation of DNA replication factors. Posttranslational modifications, such as ubiquitylation, constitute a fast and effective mechanism to control such complex protein function.
Franz, André
core
Making ends meet: repairing breaks in bacterial DNA by non-homologous end-joining. [PDF]
, 2006 DNA double-strand breaks (DSBs) are one of the most dangerous forms of DNA lesion that can result in genomic instability and cell death. Therefore cells have developed elaborate DSB-repair pathways to maintain the integrity of genomic DNA.
Bowater, Richard, Doherty, Aidan J
core +5 more sources
Advanced Biology, EarlyView.The study reveals that RRP9 is abnormally highly expressed in ESCC tissues and is closely associated with poor prognosis in patients. Furthermore, it is found that RRP9 promotes ESCC progression through enhancing the E2F1‐mediated transcriptional regulation of CDK1.
Gang He +14 more
wiley +1 more source
Profiling the selectivity of DNA ligases in an array format with mass spectrometry
Nucleic Acids Research, 2009 This article describes a method for the global profiling of the substrate specificities of DNA ligases and illustrates examples using the Taq and T4 DNA ligases.
Joohoon Kim, M. Mrksich
semanticscholar +1 more source
Advanced Healthcare Materials, EarlyView.Membrane fusion‐inspired nanomaterials offer transformative potential in diagnostics by mimicking natural fusion processes to achieve highly sensitive and specific detection of disease biomarkers. This review highlights recent advancements in nanomaterial functionalization strategies, signal amplification systems, and stimuli‐responsive fusion designs,
Sojeong Lee +9 more
wiley +1 more source
The STUbL RNF4 regulates protein group SUMOylation by targeting the SUMO conjugation machinery
Nature Communications, 2017 SUMO and ubiquitin are key signal transducers in several cellular processes including the DNA-damage response. Here the authors describe a method for selective enrichment of ubiquitin substrates for E3 ligases from complex cellular proteomes and identify
Ramesh Kumar +4 more
doaj +1 more source
Subcellular organization of UBE3A in human cerebral cortex. [PDF]
, 2018 BackgroundLoss of UBE3A causes Angelman syndrome, whereas excess UBE3A activity appears to increase the risk for autism. Despite this powerful association with neurodevelopmental disorders, there is still much to be learned about UBE3A, including its ...
Burette, Alain C +6 more
core +3 more sources