Results 301 to 310 of about 81,238 (327)

DNA ligase-AMP adducts: Identification of yeast DNA ligase polypeptides

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1985
Yeast DNA ligase is radioactively labelled in vitro by incubating a crude cell extract with [alpha-32P]ATP. The product of this reaction is the stable covalent ligase-AMP adduct, which can be characterized by its reactivity with either pyrophosphate or nicked DNA and visualized by gel electrophoresis and autoradiography.
G R, Banks, D G, Barker
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A DNA metalloenzyme with DNA ligase activity

Nature, 1995
Single-stranded DNA can fold into well-defined sequence-dependent tertiary structures that specifically bind a variety of target molecules, raising the possibility that some folded single-stranded DNAs might exhibit catalytic activities similar to those of ribozymes and protein enzymes.
B, Cuenoud, J W, Szostak
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DNA Polymerases and DNA Ligases

2013
DNA polymerase and DNA ligase are ubiquitous enzymes that synthesize complementary DNA strands according to the template DNA and ligate breaks in the backbone structure of DNA, respectively, in living organisms. Multiple enzymes have been identified from each organism, and the functional sharing of these enzymes has been investigated for both DNA ...
Sonoko Ishino, Yoshizumi Ishino
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DNA ligases in human leukemia

Leukemia Research, 1985
Following partial purification on sucrose gradient and/or phosphocellulose chromatography, DNA ligase was tested in peripheral white blood and bone marrow cells of nearly 100 patients with various kinds of leukemias, mainly acute leukemias. Terminal deoxynucleotidyl transferase (TdT) was tested in parallel.
J C, David, R, Zittoun, T, Bassez, D, Maniey, R, Rusquet, M, Bonhommet, P Y, Le Prise, D, Thevenin, A M, Suberville, J P, Marie   +9 more
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DNA ligases and ligase-based technologies

Clinical and Applied Immunology Reviews, 2001
Abstract DNA ligases catalyze the strand joining reaction at a nick junction. The requirement of base-pair complementarity at the nick junction has been explored for development of ligase-based technologies for mutation detection. In oligonucleotide ligation assay (OLA), two DNA probes complementary to the target sequence are joined by DNA ligase ...
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Ligation of DNA with T4 DNA Ligase

2003
Since they are involved in such important processes as DNA replication, DNA repair, and DNA recombination, DNA ligases can be found in all living cells. Two prokaryotic DNA ligases have become indispensible tools in the fields of in vitro DNA recombination and DNA synthesis. DNA ligase from E. coli is a polypeptide with a molecular weight of 74,000 and
W, Gaastra, K, Hansen
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1 DNA Ligases

1982
Publisher Summary DNA ligases refer to enzymes that catalyze the formation of a phosphodiester linkage between DNA chains. DNA ligases are essential reagents in studies on nucleic acid structure and metabolism. Their value derives from the specificity of the reaction and their ability to join polynucleotide chains covalently.
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Eukaryotic DNA Ligases and DNA Repair

1998
The correct joining of appropriate DNA ends is a fundamental step in many important reactions within the cell and a number of enzymes have been identified which perform these end-joining reactions. Some enzymes execute a ligation step as an intermediate of their overall reaction mechanism; DNA topoisomerases are a prime example of this type of activity
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DNA Ligases

2005
A Montecucco, R Rossi
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DNA Ligase

RCSB Protein Data Bank, 2004
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