Results 51 to 60 of about 79,069 (319)

Label-free electrochemical monitoring of DNA ligase activity [PDF]

, 2008
This study presents a simple, label-free electrochemical technique for the monitoring of DNA ligase activity. DNA ligases are enzymes that catalyze joining of breaks in the backbone of DNA and are of significant scientific interest due to their essential
Ahel I., Bellamy S. R., Borodina T. A., Bullard D. R., Cahova-Kucharikova K., Cao W. G., Doherty A. J., Fadrna R., Fojta M., Fojta M., Fojta M., Fojta M., Fojta M., Fojta M., Friedberg E. C., Gowers D. M., Heiter D. F., Kucharikova K., Liu L. F., Palecek E., Pascal J. M., Rass U., Schrock R. D., Scott B. O. S., Shuman S., Tomkinson A. E., Vacek J., Wilkinson A., Wilkinson A., Zauner G.   +29 more
core   +1 more source

SUMOylation inhibits FOXM1 activity and delays mitotic transition [PDF]

, 2014
The forkhead box transcription factor FOXM1 is an essential effector of G2/M-phase transition, mitosis and the DNA damage response. As such, it is frequently deregulated during tumorigenesis. Here we report that FOXM1 is dynamically modified by SUMO1 but
Brosens, Jan J., Coombes, R. C., Dunsby, C. W., French, P. M., Gomes, Ana R., Karunarathna, U., Kelly, D. J., Khongkow, P., Kongsema, M., Lam, Eric W.-F., Langer, J. K., Man, Cornelia W-Y., Myatt, Stephen S., Zona, Stefania   +13 more
core   +1 more source

Rat liver DNA ligases [PDF]

European Journal of Biochemistry, 1992
A novel form of rat liver DNA ligase (molecular mass 100 kDa) can be differentiated from DNA ligase I by several biochemical parameters. It is a more heat‐labile enzyme and unable to join bluntended DNA, even in the presence of poly(ethylene glycol) concentrations which stimulate such joining by DNA ligase I and T4 DNA ligase.
R H Elder, A Montecucco, G Ciarrocchi, J M Rossignol   +3 more
openaire   +4 more sources

The Action of DNA Ligase at Abasic Sites in DNA [PDF]

Journal of Biological Chemistry, 1998
Apurinic/apyrimidinic (AP) sites occur frequently in DNA as a result of spontaneous base loss or following removal of a damaged base by a DNA glycosylase. The action of many AP endonuclease enzymes at abasic sites in DNA leaves a 5'-deoxyribose phosphate (dRP) residue that must be removed during the base excision repair process.
Daniel F. Bogenhagen, Kevin G. Pinz
openaire   +2 more sources

BioE3 identifies specific substrates of ubiquitin E3 ligases

Nature Communications, 2023
Hundreds of E3 ligases play a critical role in recognizing specific substrates for modification by ubiquitin (Ub). Separating genuine targets of E3s from E3-interactors remains a challenge. We present BioE3, a powerful approach for matching substrates to
Orhi Barroso-Gomila, Laura Merino-Cacho, Veronica Muratore, Coralia Perez, Vincenzo Taibi, Elena Maspero, Mikel Azkargorta, Ibon Iloro, Fredrik Trulsson, Alfred C. O. Vertegaal, Ugo Mayor, Felix Elortza, Simona Polo, Rosa Barrio, James D. Sutherland   +14 more
doaj   +1 more source

Closing the gap on DNA ligase [PDF]

Structure, 1996
The crystal structure of T7 DNA ligase complexed with ATP illuminates the mechanism of covalent catalysis by a superfamily of nucleotidyl transferases that includes the ATP-dependent polynucleotide ligases and the GTP-dependent mRNA capping enzymes.
openaire   +3 more sources

Parkin-independent mitophagy controls chemotherapeutic response in cancer cells [PDF]

, 2017
Mitophagy is an evolutionarily conserved process that selectively targets impaired mitochondria for degradation. Defects in mitophagy are often associated with diverse pathologies, including cancer.
Bossowski, Jozef P., Chiche, Johanna, Marchetti, Sandrine, Mondragón, Laura, Obba, Sandrine, Proïcs, Emma, Ricci, Jean-Ehrland, Riley, Joel S., Rozier, Romain M., Rubio-Patiño, Camila, Tait, Stephen W.G., Verhoeyen, Els, Villa, Elodie, Zunino, Barbara   +13 more
core   +2 more sources

Two-tiered enforcement of high-fidelity DNA ligation

Nature Communications, 2019
DNA ligases catalyze the joining of DNA strands to complete DNA replication, recombination and repair transactions. Here the authors present X-ray structures and kinetic analyses of LIG1 complexes with undamaged and oxidatively damaged DNA that unveil ...
Percy P. Tumbale, Thomas J. Jurkiw, Matthew J. Schellenberg, Amanda A. Riccio, Patrick J O’Brien, R. Scott Williams   +5 more
doaj   +1 more source

Identification of novel components of Trypanosoma brucei editosomes [PDF]

, 2003
The editosome is a multiprotein complex that catalyzes the insertion and deletion of uridylates that occurs during RNA editing in trypanosomatids. We report the identification of nine novel editosome proteins in Trypanosoma brucei.
Carmean, Nicole, Ernst, Nancy L, Panigrahi, Aswini K, Salavati, Reza, Schnaufer, Achim, Stuart, Kenneth, Wang, Bingbing   +6 more
core   +2 more sources

Global-genome Nucleotide Excision Repair Controlled by Ubiquitin/Sumo Modifiers

Frontiers in Genetics, 2016
Global-genome nucleotide excision repair (GG-NER) prevents genome instability by excising a wide range of structurally unrelated DNA base adducts and crosslinks induced by chemical carcinogens, ultraviolet (UV) radiation or intracellular metabolic by ...
Peter eRuethemann, Chiara eBalbo Pogliano, Hanspeter eNaegeli   +2 more
doaj   +1 more source