Results 61 to 70 of about 77,898 (323)

Label-free electrochemical monitoring of DNA ligase activity [PDF]

, 2008
This study presents a simple, label-free electrochemical technique for the monitoring of DNA ligase activity. DNA ligases are enzymes that catalyze joining of breaks in the backbone of DNA and are of significant scientific interest due to their essential
Ahel I., Bellamy S. R., Borodina T. A., Bullard D. R., Cahova-Kucharikova K., Cao W. G., Doherty A. J., Fadrna R., Fojta M., Fojta M., Fojta M., Fojta M., Fojta M., Fojta M., Friedberg E. C., Gowers D. M., Heiter D. F., Kucharikova K., Liu L. F., Palecek E., Pascal J. M., Rass U., Schrock R. D., Scott B. O. S., Shuman S., Tomkinson A. E., Vacek J., Wilkinson A., Wilkinson A., Zauner G.   +29 more
core   +1 more source

TRAF2 binds to TIFA via a novel motif and contributes to its autophagic degradation

FEBS Letters, EarlyView.
TRAF family members couple receptor signalling complexes to downstream outputs, but how they interact with these complexes is not always clear. Here, we show that during ADP‐heptose signalling, TRAF2 binding to TIFA requires two short sequence motifs in the C‐terminal tail of TIFA, which are distinct from the TRAF6 binding motif.
Tom Snelling, Kodi Hunter, Celest W. S. Tay, Nicola T. Wood, Philip Cohen   +4 more
wiley   +1 more source

Real estate in the DNA damage response: Ubiquitin and SUMO ligases home in on DNA double-strand breaks

Frontiers in Genetics, 2016
Ubiquitin and the ubiquitin-like modifier SUMO are intimately connected with the cellular response to various types of DNA damage. A striking feature is the local accumulation of these proteinaceous post-translational modifications in the direct vicinity
Nico P. Dantuma, Annika ePfeiffer
doaj   +1 more source

The DNA binding domain and the C-terminal region of DNA Ligase IV specify its role in V(D)J recombination.

PLoS ONE, 2023
DNA Ligase IV is responsible for the repair of DNA double-strand breaks (DSB), including DSBs that are generated during V(D)J recombination. Like other DNA ligases, Ligase IV contains a catalytic core with three subdomains-the DNA binding (DBD), the ...
Vidyasagar Malashetty, Audrey Au, Jose Chavez, Mary Hanna, Jennifer Chu, Jesse Penna, Patricia Cortes   +6 more
doaj   +1 more source

From omics to AI—mapping the pathogenic pathways in type 2 diabetes

FEBS Letters, EarlyView.
Integrating multi‐omics data with AI‐based modelling (unsupervised and supervised machine learning) identify optimal patient clusters, informing AI‐driven accurate risk stratification. Digital twins simulate individual trajectories in real time, guiding precision medicine by matching patients to targeted therapies.
Siobhán O'Sullivan, Lu Qi, Pierre Zalloua   +2 more
wiley   +1 more source

RNAi-Based Suppressor Screens Reveal Genetic Interactions Between the CRL2LRR-1 E3-Ligase and the DNA Replication Machinery in Caenorhabditis elegans

G3: Genes, Genomes, Genetics, 2016
Cullin-RING E3-Ligases (CRLs), the largest family of E3 ubiquitin-Ligases, regulate diverse cellular processes by promoting ubiquitination of target proteins.
Batool Ossareh-Nazari, Anthi Katsiarimpa, Jorge Merlet, Lionel Pintard   +3 more
doaj   +1 more source

Structural insights into Cullin4-RING ubiquitin ligase remodelling by Vpr from simian immunodeficiency viruses.

PLoS Pathogens, 2021
Viruses have evolved means to manipulate the host's ubiquitin-proteasome system, in order to down-regulate antiviral host factors. The Vpx/Vpr family of lentiviral accessory proteins usurp the substrate receptor DCAF1 of host Cullin4-RING ligases (CRL4),
Sofia Banchenko, Ferdinand Krupp, Christine Gotthold, Jörg Bürger, Andrea Graziadei, Francis J O'Reilly, Ludwig Sinn, Olga Ruda, Juri Rappsilber, Christian M T Spahn, Thorsten Mielke, Ian A Taylor, David Schwefel   +12 more
doaj   +1 more source

An improved protocol for small RNA library construction using High Definition adapters [PDF]

, 2015
Next generation sequencing of small RNA (sRNA) libraries is widely used for studying sRNAs in various biological systems. However, cDNA libraries of sRNAs are biased for molecules that are ligated to adapters more or less efficiently than other molecules.
Aravin, Baran, Bushati, Edgar, Git, Hafner, He, Jagadeeswaran, Jayaprakash, Kawano, Mallory, Mortazavi, Munafó, Pease, Prüfer, Raabe, Reddy, Song, Sorefan, Stocks, Sun, Szittya, Szittya, Tian, Vigneault, Viollet, Willenbrock, Zhang, Zhuang   +28 more
core   +1 more source

Exploring lipid diversity and minimalism to define membrane requirements for synthetic cells

FEBS Letters, EarlyView.
Designing the lipid membrane of synthetic cells is a complex task, in which its various roles (among them solute transport, membrane protein support, and self‐replication) should all be integrated. In this review, we report the latest top‐down and bottom‐up advances and discuss compatibility and complexity issues of current engineering approaches ...
Sergiy Gan, Victoria Scarpelli, Marten Exterkate   +2 more
wiley   +1 more source

Cullin 3 as a novel target in diverse pathologies

Redox Biology, 2013
Recent evidence suggests that the malfunctioning disposal system of cell protein called ubiquitin–proteasome system (UPS) plays an important role in the development of disorders, including cancer and neurodegenerative diseases.
Ana Cristina Andérica-Romero, Irma Gabriela González-Herrera, Abel Santamaría, José Pedraza-Chaverri   +3 more
doaj   +1 more source