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DNA metabarcode choice and design
2018Abstract Chapter “DNA metabarcode choice and design” develops the properties of the ideal metabarcode in a given context, including conservation of the primer annealing regions and resolution power across the target taxonomic group of interest.
Pierre Taberlet +3 more
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A DNA Metabarcoding Workflow to Identify Species in Spices and Herbs
Journal of AOAC INTERNATIONAL, 2022Abstract Background Spices and herbs are food categories regularly cited as highly susceptible to be adulterated. To detect potential adulteration with undeclared species, DNA-based methods are considered the most suitable tools. Objective
Geoffrey Cottenet +7 more
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Environmental DNA and RNA Metabarcoding
Environmental DNA (eDNA) and RNA (eRNA) technologies, when integrated with metabarcoding, have emerged as significant instruments in cancer research particularly in exposomics and environmental carcinogenesis. These approaches facilitate the identification and monitoring of genetic material from many environmental sources, yielding insights into the ...Odangowei Inetiminebi Ogidi +2 more
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DNA Barcoding and Metabarcoding Protocols for Species Identification
The article presents the several steps to be performed on a plant, fungal, insect, or soil sample to obtain DNA sequences for DNA barcode analysis. The chapter begins with a description of sample preparation including procedures for cleaning and proceeds to DNA extraction with methods adapted for the specific type of sample. Next, DNA quantification isKyleen E, Elwick +2 more
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Testing the applicability of environmental DNA metabarcoding to landscape genetics
Molecular Ecology ResourcesAbstractLandscape genetics is a field dealing with local genetic differences and contributes to strategic conservation planning. Recently, environmental DNA (eDNA) metabarcoding has proven useful not only for detecting species but also for assessing genetic diversity and genetic structure on a large scale such as in phylogeography.
Souta Nakajima, Kenji Tsuri
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