Results 251 to 260 of about 259,719 (301)

Plant DNA methyltransferases

Plant Molecular Biology, 2000
DNA methylation is an important modification of DNA that plays a role in genome management and in regulating gene expression during development. Methylation is carried out by DNA methyltransferases which catalyse the transfer of a methyl group to bases within the DNA helix.
E J, Finnegan, K A, Kovac
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EcoRI DNA methyltransferase-DNA interactions

Biochemistry, 1992
We present a novel strategy with synthetic hemimethylated DNA substrates containing uracil for thymine and inosine for guanosine replacements and EcoRI DNA methyltransferase to characterize the importance of major groove hydrophobic groups to the sequence-specific modification of DNA.
N O, Reich, M J, Danzitz
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Sea urchin DNA methyltransferases

Cell Biophysics, 1989
DNA methyltransferase activities have been partially purified from unfertilized eggs and blastula nuclei of sea urchin embryos. Comparative studies, using different DNAs as substrates, show that the two preparations are most active on hemimethylated and single-strand DNA, but they methylate, though at a lower rate, also on double-strand DNA.
Tosi L, Tomei L, Branno M, FUGGI, Amodio, Aniello F, Geraci G.   +5 more
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DNA Methyltransferases and DNA Damage

2022
Ever since the discovery of depletion of CG sites in mammalian genomes it has been clear that cytosine DNA methyltransferases (DNMTs) are linked to the rate at which mutations accumulate in DNA. Research in the intervening decades has shown that DNMTs influence mutation rates through the indirect consequences of methylation on the mechanism of mutation
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DNA Methyltransferase Assays

2011
DNA methyltransferases are important enzymes and their inhibition has many potential applications. The investigation of DNA methyltransferases as well as screening for potential inhibitors requires specialized enzyme assays. In this chapter, we describe three DNA methyltransferase assays, each of them based on a different method: (1) An assay using ...
Renata Z, Jurkowska, Alexandre, Ceccaldi, Yingying, Zhang, Paola B, Arimondo, Albert, Jeltsch   +4 more
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Microassay for DNA methyltransferase

Journal of Biochemical and Biophysical Methods, 1991
A microassay for DNA methylase is described which can detect activity in as few as 50 tissue culture cells. The cells are lysed and incubated for 2 h at 37 degrees C with 3 microCi high specific activity [3H]AdoMet and 0.5 microgram poly[d(I-C).d(I-C)] in a volume of 23 microliters.
R L, Adams, A, Rinaldi, C, Seivwright
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DNA Labeling Using DNA Methyltransferases

2016
DNA methyltransferases (MTases) uniquely combine the ability to recognize and covalently modify specific target sequences in DNA using the ubiquitous cofactor S-adenosyl-L-methionine (AdoMet). Although DNA methylation plays important roles in biological signaling, the transferred methyl group is a poor reporter and is highly inert to further ...
Tomkuvienė, Miglė, Kriukienė, Edita, Klimašauskas, Saulius   +2 more
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DNA modification by methyltransferases

Current Opinion in Structural Biology, 1995
Enzymatic methylation of DNA plays important roles in both prokaryotes and eukaryotes. Structural study of the HhaI DNA methyltransferase has provided considerable insight into the chemistry of C5-cytosine methylation. The DNA-protein complex reveals a substrate cytosine flipped out of the double helix during the reaction, and a novel two-loop DNA ...
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DNA Methyltransferase Probing of DNA-Protein Interactions

2006
Effective methods of probing chromatin structure without disrupting DNA-protein interactions and associations are necessary for creating an accurate picture of chromatin and its processes in vivo. Expression of cytidine-5 DNA methyltransferases (C5 DMTases) in Saccharomyces cerevisiae provides a powerful noninvasive method of assaying relative DNA ...
Scott A, Hoose, Michael P, Kladde
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DNA Methyltransferase-Moderated Click Chemistry

Organic Letters, 2005
[reaction: see text]. Biological methylation plays a vital role in regulatory mechanisms of gene transcription. Methylation of both promoter sequences within the genome, as well as protein substrates, has a profound impact upon gene transcription. Yet, few tools exist by which to identify sites of biological methylation in complex biological mixtures ...
Rachel L, Weller, Scott R, Rajski
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