Results 1 to 10 of about 95,321 (191)
A bacterial defense system targeting modified cytosine of phage genomic DNA [PDF]
Nature CommunicationsThe evolutionary arms race between bacteria and phages drives the development of bacterial antiviral defense systems and phage counter-defense strategies.
Rui Liu +6 more
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The Impact of the HydroxyMethylCytosine epigenetic signature on DNA structure and function
PLoS Computational Biology, 2021 We present a comprehensive, experimental and theoretical study of the impact of 5-hydroxymethylation of DNA cytosine. Using molecular dynamics, biophysical experiments and NMR spectroscopy, we found that Ten-Eleven translocation (TET) dioxygenases ...
Federica Battistini +9 more
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The DNA (cytosine-5) methyltransferases [PDF]
Nucleic Acids Research, 1994 The m5C-MTases form a closely-knit family of enzymes in which common amino acid sequence motifs almost certainly translate into common structural and functional elements. These common elements are located predominantly in a single structural domain that performs the chemistry of the reaction.
S, Kumar +6 more
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The behaviour of 5-hydroxymethylcytosine in bisulfite sequencing. [PDF]
PLoS ONE, 2010 We recently showed that enzymes of the TET family convert 5-mC to 5-hydroxymethylcytosine (5-hmC) in DNA. 5-hmC is present at high levels in embryonic stem cells and Purkinje neurons.
Yun Huang +5 more
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A mutant of uracil DNA glycosylase that distinguishes between cytosine and 5-methylcytosine. [PDF]
PLoS ONE, 2014 We demonstrate that a mutant of uracil DNA glycosylase (N123D:L191A) distinguishes between cytosine and methylcytosine. Uracil DNA glycosylase (UDG) efficiently removes uracil from DNA in a reaction in which the base is flipped into the enzyme's active ...
Scott T Kimber, Tom Brown, Keith R Fox
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Structure of mouse DNA (cytosine-5-)-methyltransferase [PDF]
European Journal of Biochemistry, 1988 DNA (cytosine‐5‐)‐methyltransferase was purified as a single polypeptide (190 kDa by SDS‐PAGE) from mouse P815 mastocytoma cells. This enzyme transfers methyl groups to unmethylated as well as to hemimethylated DNA sites with a strong preference for the hemimethylated substrate.
E, Spiess +3 more
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Acceleration of the Deamination of Cytosine through Photo-Crosslinking
Current Issues in Molecular Biology, 2023 Herein, we report the major factor for deamination reaction rate acceleration, i.e., hydrophilicity, by using various 5-substituted target cytosines and by carrying out deamination at high temperatures.
Siddhant Sethi +5 more
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DNA bending induced by DNA (cytosine-5) methyltransferases [PDF]
Nucleic Acids Research, 2000 DNA bending induced by six DNA (cytosine-5) methyltransferases was studied using circular permutation gel mobility shift assay. The following bend angles were obtained: M.BSP:RI (GG(m5)CC), 46-50 degrees; M.HAE:III (GG(m5)CC), 40-43 degrees; M.SIN:I (GGW(m5)CC), 34-37 degrees; M.SAU:96I (GGN(m5)CC), 52-57 degrees; M.HPA:II (C(m5)CGG), 30 degrees; and M.
T, Raskó, C, Finta, A, Kiss
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Epigenetics, 2021 The presence of 5-hydroxymethyl cytosine in DNA has been previously associated with ageing. Using in silico analysis of normal liver samples we presently observed that in 5-hydroxymethyl cytosine sequences, DNA methylation is dependent on the co-presence
Andigoni Malousi +2 more
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eLife, 2021 Shotgun metagenomic sequencing is a powerful approach to study microbiomes in an unbiased manner and of increasing relevance for identifying novel enzymatic functions.
Weiwei Yang +9 more
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