Results 1 to 10 of about 94,067 (257)
MeDReaders 2.0: an updated database for modified DNA readers [PDF]
BMC GenomicsHere, we present a significant update to the MeDReaders database and webserver. The new MeDReaders 2.0 covers interactions between transcription factors (TFs) and DNA cytosine modifications that arise during DNA methylation and active demethylation ...
Hongfei Li +3 more
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A bacterial defense system targeting modified cytosine of phage genomic DNA [PDF]
Nature CommunicationsThe evolutionary arms race between bacteria and phages drives the development of bacterial antiviral defense systems and phage counter-defense strategies.
Rui Liu +6 more
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The Impact of the HydroxyMethylCytosine epigenetic signature on DNA structure and function
PLoS Computational Biology, 2021 We present a comprehensive, experimental and theoretical study of the impact of 5-hydroxymethylation of DNA cytosine. Using molecular dynamics, biophysical experiments and NMR spectroscopy, we found that Ten-Eleven translocation (TET) dioxygenases ...
Federica Battistini +9 more
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Structure of mouse DNA (cytosine-5-)-methyltransferase [PDF]
European Journal of Biochemistry, 1988 DNA (cytosine‐5‐)‐methyltransferase was purified as a single polypeptide (190 kDa by SDS‐PAGE) from mouse P815 mastocytoma cells. This enzyme transfers methyl groups to unmethylated as well as to hemimethylated DNA sites with a strong preference for the hemimethylated substrate.
E, Spiess +3 more
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The behaviour of 5-hydroxymethylcytosine in bisulfite sequencing. [PDF]
PLoS ONE, 2010 We recently showed that enzymes of the TET family convert 5-mC to 5-hydroxymethylcytosine (5-hmC) in DNA. 5-hmC is present at high levels in embryonic stem cells and Purkinje neurons.
Yun Huang +5 more
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A mutant of uracil DNA glycosylase that distinguishes between cytosine and 5-methylcytosine. [PDF]
PLoS ONE, 2014 We demonstrate that a mutant of uracil DNA glycosylase (N123D:L191A) distinguishes between cytosine and methylcytosine. Uracil DNA glycosylase (UDG) efficiently removes uracil from DNA in a reaction in which the base is flipped into the enzyme's active ...
Scott T Kimber, Tom Brown, Keith R Fox
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DNA bending induced by DNA (cytosine-5) methyltransferases [PDF]
Nucleic Acids Research, 2000 DNA bending induced by six DNA (cytosine-5) methyltransferases was studied using circular permutation gel mobility shift assay. The following bend angles were obtained: M.BSP:RI (GG(m5)CC), 46-50 degrees; M.HAE:III (GG(m5)CC), 40-43 degrees; M.SIN:I (GGW(m5)CC), 34-37 degrees; M.SAU:96I (GGN(m5)CC), 52-57 degrees; M.HPA:II (C(m5)CGG), 30 degrees; and M.
T, Raskó, C, Finta, A, Kiss
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Epigenetics, 2021 The presence of 5-hydroxymethyl cytosine in DNA has been previously associated with ageing. Using in silico analysis of normal liver samples we presently observed that in 5-hydroxymethyl cytosine sequences, DNA methylation is dependent on the co-presence
Andigoni Malousi +2 more
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Acceleration of the Deamination of Cytosine through Photo-Crosslinking
Current Issues in Molecular Biology, 2023 Herein, we report the major factor for deamination reaction rate acceleration, i.e., hydrophilicity, by using various 5-substituted target cytosines and by carrying out deamination at high temperatures.
Siddhant Sethi +5 more
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An orphan DNA (cytosine-5-)-methyltransferase in Vibrio cholerae [PDF]
Microbiology, 2006 5-Methyl cytosine (m5C) was detected in genomic DNA of the enteric pathogen Vibrio cholerae by HPLC analysis and immunoblotting with m5C-specific antibody. Although cleavage with the restriction endonuclease EcoRII revealed the absence of a Dcm homologue in V.
Sanjib, Banerjee, Rukhsana, Chowdhury
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