Results 31 to 40 of about 95,440 (308)
An orphan DNA (cytosine-5-)-methyltransferase in Vibrio cholerae [PDF]
Microbiology, 2006 5-Methyl cytosine (m5C) was detected in genomic DNA of the enteric pathogen Vibrio cholerae by HPLC analysis and immunoblotting with m5C-specific antibody. Although cleavage with the restriction endonuclease EcoRII revealed the absence of a Dcm homologue in V.
Sanjib, Banerjee, Rukhsana, Chowdhury
openaire +2 more sources
Fluorescent Method for the Detection of Biothiols Using an Ag+-Mediated Conformational Switch
Sensors, 2019 In this work, a novel, simple, and time-saving fluorescence approach for the detection of biothiols (glutathione and cysteine) was developed by employing a DNA probe labeled with 2-aminopurine.
Han Zhao, Mingjian Chen, Changbei Ma
doaj +1 more source
5-hydroxymethyl-cytosine enrichment of non-committed cells is not a universal feature of vertebrate development [PDF]
, 2012 5-hydroxymethyl-cytosine (5-hmc) is a cytosine modification that is relatively abundant in mammalian pre-implantation embryos and embryonic stem cells (Esc) derived from mammalian blastocysts.
Alexey Ruzov +41 more
core +2 more sources
PLoS ONE, 2014 The epigenetic DNA modifications 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) in eukaryotes are recognized either in the context of double-stranded DNA (e.g., by the methyl-CpG binding domain of MeCP2), or in the flipped-out state (e.g., by ...
Evelina Zagorskaitė +1 more
doaj +1 more source
Analysis of early changes in DNA methylation in synovial fibroblasts of RA patients before diagnosis [PDF]
, 2018 DNA methylation is an important epigenetic modification that is known to be altered in rheumatoid arthritis synovial fibroblasts (RASF). Here, we compared the status of promoter DNA methylation of SF from patients with very early RA with SF from patients
Buckley, Christopher D +6 more
core +4 more sources
15N NMR study of a mixture of uniformly labeled tRNAs [PDF]
, 1982 15N NMR spectra were taken of 15N-enriched tRNA extracted from bakers yeast; ammonium sulfate was used as a nitrogen source. The increase in the degree of denaturation of tRNA, which occurs with increase in temperature from 30 degrees C to 70 degrees C ...
Birdseye, Theresa R. +3 more
core +1 more source
Protein-facilitated base flipping in DNA by cytosine-5-methyltransferase [PDF]
Proceedings of the National Academy of Sciences, 2002 DNA methylation, various DNA repair mechanisms, and possibly early events in the opening of DNA as required for transcription and replication are initiated by flipping of a DNA base out of the DNA double helix. The energetics and structural mechanism of base flipping in the presence of the DNA-processing enzyme, cytosine 5 ...
Niu, Huang +2 more
openaire +2 more sources
ComputationMetal-mediated base pairs in DNA double helix molecules open up broad opportunities for biosensors based on DNA clusters with silver due to their low toxicity and applicability in drug design.
Elena B. Gusarova, Natalya A. Kovaleva
doaj +1 more source
Environmentally sensitive molecular probes reveal mutations and epigenetic 5-methyl cytosine in human oncogenes [PDF]
, 2017 There is currently an unmet need for reliable tools that allow for direct detection and quantification of modifications in genomic DNA.
Astakhova, K. +2 more
core +2 more sources
Human Amniocytes Are Receptive to Chemically Induced Reprogramming to Pluripotency [PDF]
, 2017 Restoring pluripotency using chemical compounds alone would be a major step forward in developing clinical-grade pluripotent stem cells, but this has not yet been reported in human cells.
Adjaye, J +15 more
core +4 more sources