Results 51 to 60 of about 95,440 (308)

Bypass of a protein roadblock by a replicative DNA helicase [PDF]

, 2014
Replicative DNA helicases generally unwind DNA as a single hexamer that encircles and translocates along one strand of the duplex while excluding the complementary strand (“steric exclusion”).
Hurwitz, Jerard, Loveland, Anna B., Rudner, David Z., Tappin, Inger, van Oijen, Antoine M., Walter, Johannes C., Wang, Xindan, Yardimci, Hasan   +7 more
core   +1 more source

Crucial parameters for precise copy number variation detection in formalin‐fixed paraffin‐embedded solid cancer samples

Molecular Oncology, EarlyView.
This study shows that copy number variations (CNVs) can be reliably detected in formalin‐fixed paraffin‐embedded (FFPE) solid cancer samples using ultra‐low‐pass whole‐genome sequencing, provided that key (pre)‐analytical parameters are optimized.
Hanne Goris, Vasiliki Siozopoulou, Léon C van Kempen, Anne Sieben, Ella Roelant, Stig Hellemans, Elyne Backx, Laure Sorber, Koen De Winne, Senada Koljenović, Karen Zwaenepoel   +10 more
wiley   +1 more source

The Effect of Sulphur Atom on the Structure of Biomolecule 2-Thiocytosine in the Gas-Phase, Solid-State, and Hydrated Forms and in DNA–DNA Microhelices as Compared to Canonical Ones

Molecules
This study is focused on the effects of the sulphur atom in position 2 of the cytosine molecule, 2-thiocytosine (2TC), on the molecular structural parameters in the isolated state, as well as in the hydration, solid state arrangement, Watson–Crick pairs,
Mauricio Alcolea Palafox, Valentin Alba Aparicio, Sergio Toninelli Rodriguez, Josefa Isasi Marín, Jitendra Kumar Vats, Vinod Kumar Rastogi   +5 more
doaj   +1 more source

MiR‐513a promotes human erythroid differentiation by modulating c‐Jun

FEBS Open Bio, EarlyView.
During early human erythropoiesis, miR‐513a promoted erythroid differentiation in primary human CD34+ hematopoietic stem‐progenitor cells and human TF‐1 erythroleukemic cells by indirectly decreasing c‐Jun and phospho‐c‐Jun expression, which are associated with increased GATA1 expression.
MinJung Kim, Brittany Taylor, Shannon Bolten, Christian L. Eberly, Mahliya Abdurahman, T. Michael Creed, Acong Yang, Taylor L. Hatchet, Tristan Dyson, Shuo Gu, Curt I. Civin, Tami J. Kingsbury   +11 more
wiley   +1 more source

Mutation of a major CG methylase alters genome-wide lncRNA expression in rice

G3: Genes, Genomes, Genetics, 2021
Plant long non-coding RNAs (lncRNAs) function in diverse biological processes, and lncRNA expression is under epigenetic regulation, including by cytosine DNA methylation. However, it remains unclear whether 5-methylcytosine (5mOsMET1-2OsMET1-2−
Juzuo Li, Ning Li, Ling Zhu, Zhibin Zhang, Xiaochong Li, Jinbin Wang, Hongwei Xun, Jing Zhao, Xiaofei Wang, Tianya Wang, Hongyan Wang, Bao Liu, Yu Li, Lei Gong   +13 more
doaj   +1 more source

Engineering the DNA cytosine-5 methyltransferase reaction for sequence-specific labeling of DNA [PDF]

Nucleic Acids Research, 2012
DNA methyltransferases catalyse the transfer of a methyl group from the ubiquitous cofactor S-adenosyl-L-methionine (AdoMet) onto specific target sites on DNA and play important roles in organisms from bacteria to humans. AdoMet analogs with extended propargylic side chains have been chemically produced for methyltransferase-directed transfer of ...
G. Lukinavicius, A. Lapinaite, G. Urbanaviciute, R. Gerasimaite, S. Klimasauskas   +4 more
openaire   +4 more sources

RNA‐Micelles as Self‐Assembling Structures for Efficient Co‐Delivery of Synergistic siRNA and Nucleoside Analogues to Treat CRC Lung Metastasis

Advanced Functional Materials, EarlyView.
Two kinds of self‐assembled RNA micelles were used to co‐deliver synergistic siRNA and nucleoside analogues for the treatment of colorectal cancer lung metastases. Near‐complete elimination of lung cancer metastases was confirmed in an orthotopic lung metastasis model constructed using human colorectal cancer lung metastases patient surgical samples to
Kai Jin, Piotr Rychahou, Daniel W. Binzel, B. Mark Evers, Peixuan Guo   +4 more
wiley   +1 more source

A plant-based diet differentially affects the global hepatic methylome in rainbow trout depending on genetic background

Epigenetics, 2022
Replacing fish meal and oil in trout diets with plant-derived ingredients is a contemporary challenge to move towards more sustainable aquaculture practices.
Marandel L, Heraud C, Véron V, Laithier J, Marchand M, Quillet E, Callet T, Dupont-Nivet M, Médale F   +8 more
doaj   +1 more source

Use of a molecular beacon based fluorescent method for assaying uracil DNA glycosylase (Ung) activity and inhibitor screening

Biochemistry and Biophysics Reports, 2021
Uracil DNA glycosylases are an important class of enzymes that hydrolyze the N-glycosidic bond between the uracil base and the deoxyribose sugar to initiate uracil excision repair.
Avani Mehta, Prateek Raj, Sandeep Sundriyal, Balasubramanian Gopal, Umesh Varshney   +4 more
doaj   +1 more source

Mammalian DNA (Cytosine-5-)-methyltransferase Expressed in Escherichia coli, Purified and Characterized [PDF]

Journal of Biological Chemistry, 1995
Besides modulating specific DNA-protein interactions, methylated cytosine, frequently referred to as the fifth base of the genome, also influences DNA structure, recombination, transposition, repair, transcription, imprinting, and mutagenesis. DNA (cytosine-5-)-methyltransferase catalyzes cytosine methylation in eukaryotes. We have cloned and expressed
T O, Tollefsbol, C A, Hutchison
openaire   +2 more sources