Results 51 to 60 of about 94,090 (163)

Elevated 5hmC levels characterize DNA of the cerebellum in Parkinson’s disease

npj Parkinson's Disease, 2017
5-methylcytosine and the oxidation product 5-hydroxymethylcytosine are two prominent epigenetic variants of the cytosine base in nuclear DNA of mammalian brains.
Reinhard Stöger, Paula J. Scaife, Freya Shephard, Lisa Chakrabarti   +3 more
doaj   +1 more source

DNA methylation: a timeline of methods and applications.

Frontiers in Genetics, 2011
DNA methylation is a biochemical process where a DNA base, usually cytosine, is enzymatically methylated at the 5-carbon position. An epigenetic modification associated with gene regulation, DNA methylation is of paramount importance regarding biological
Anne eParle-Mcdermott, Alan eHarrison
doaj   +1 more source

Accurate estimation of 5-methylcytosine in mammalian mitochondrial DNA

Scientific Reports, 2018
Whilst 5-methylcytosine (5mC) is a major epigenetic mark in the nuclear DNA in mammals, whether or not mitochondrial DNA (mtDNA) receives 5mC modification remains controversial.
Shigeru Matsuda, Takehiro Yasukawa, Yuriko Sakaguchi, Kenji Ichiyanagi, Motoko Unoki, Kazuhito Gotoh, Kei Fukuda, Hiroyuki Sasaki, Tsutomu Suzuki, Dongchon Kang   +9 more
doaj   +1 more source

Irradiation and Alterations in Hippocampal DNA Methylation

Epigenomes
The response of the brain to radiation is important for cancer patients receiving whole or partial brain irradiation or total body irradiation, those exposed to irradiation as part of a nuclear accident or a nuclear war or terrorism event, and for ...
Soren Impey, Jacob Raber
doaj   +1 more source

Enhanced C‐To‐T and A‐To‐G Base Editing in Mitochondrial DNA with Engineered DdCBE and TALED

Advanced Science
Mitochondrial base editing with DddA‐derived cytosine base editor (DdCBE) is limited in the accessible target sequences and modest activity. Here, the optimized DdCBE tools is presented with improved editing activity and expanded C‐to‐T targeting scope ...
Yinghui Wei, Ming Jin, Shuhong Huang, Fangyao Yao, Ningxin Ren, Kun Xu, Shangpu Li, Pengfei Gao, Yingsi Zhou, Yulin Chen, Hui Yang, Wen Li, Chunlong Xu, Meiling Zhang, Xiaolong Wang   +14 more
doaj   +1 more source

Molecular Cloning, Expression, and Characterization of Starfish DNA (Cytosine-5)-methyltransferases

Bioscience, Biotechnology, and Biochemistry, 2012
To determine whether and if so how a DNA methylation-dependent epigenetic mechanism for transcriptional gene silencing functions in Echinoderms, we cloned and sequenced dnmt1 and dnmt3 cDNAs of the starfish Asterina pectinifera. Since the Strongylocentrotus purpuratus genome has only two loci of DNA (cytosine-5)-methyltransferase genes encoding Dnmt1 ...
FUJIHARA, Yoshihito, MIYASAKO, Hiroshi, KATO, Kumiko, HAYASHI, Tadahiro, TORAYA, Tetsuo   +4 more
openaire   +2 more sources

Chloroplast DNA methylation in the kelp Saccharina latissima is determined by origin and possibly influenced by cultivation

Evolutionary Applications
DNA cytosine methylation is an important epigenetic mechanism in genomic DNA. In most land plants, it is absent in the chloroplast DNA. We detected methylation in the chloroplast DNA of the kelp Saccharina latissima, a non‐model macroalgal species of ...
Lydia Scheschonk, Anne M. L. Nilsen, Kai Bischof, Alexander Jueterbock   +3 more
doaj   +1 more source

New Insights into 5hmC DNA Modification: Generation, Distribution and Function

Frontiers in Genetics, 2017
Dynamic DNA modifications, such as methylation/demethylation on cytosine, are major epigenetic mechanisms to modulate gene expression in both eukaryotes and prokaryotes.
Dong-Qiao Shi, Iftikhar Ali, Jun Tang, Wei-Cai Yang   +3 more
doaj   +1 more source

Structural basis for double-stranded DNA cytosine deamination by BaDTF3 and its application in mitochondrial genome editing

Nature Communications
Bacterial deaminase toxin family (BaDTF) proteins are weapons used in bacterial warfare, and they are useful tools in base editing, epigenetics analyses, and genomic footprinting applications.
Lulu Yin, Chae Jin Lim, Ke Shi, Jae Hee Yoon, Bong-Kook Ko, Seungmin Ryou, Jin-Soo Kim, Hideki Aihara   +7 more
doaj   +1 more source

Virus-encoded glycosyltransferases hypermodify DNA with diverse glycans

Cell Reports
Summary: Enzymatic modification of DNA nucleobases can coordinate gene expression, nuclease protection, or mutagenesis. We recently discovered a clade of phage-specific cytosine methyltransferase (MT) and 5-methylpyrimidine dioxygenase (5mYOX) enzymes ...
Jesse D. Pyle, Sean R. Lund, Katherine H. O’Toole, Lana Saleh   +3 more
doaj   +1 more source