Results 41 to 50 of about 94,090 (163)
Scientific Reports, 2018 Streptomyces coelicolor is a Gram-positive microorganism often used as a model of physiological and morphological differentiation in streptomycetes, prolific producers of secondary metabolites with important biological activities.
Annalisa Pisciotta +2 more
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Cell Reports, 2015 Ten eleven translocation (Tet) proteins oxidize 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC). 5fC and 5caC can be further excised by thymine-DNA glycosylase (Tdg).
Francesco Neri +8 more
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Epigenetic machinery is functionally conserved in cephalopods
BMC Biology, 2022 Background Epigenetic regulatory mechanisms are divergent across the animal kingdom, yet these mechanisms are not well studied in non-model organisms.
Filippo Macchi +2 more
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Determination of Methylation Site of DNA-Methyltransferase NlaX by a Hybrid Method
BioTechniques, 2002 Using a new method based on a combination of bisulfite reaction, the repair enzyme uracil-DNA glycosylase, and synthetic oligodeoxyribonucleotides, the methylation site of DNA-methyltransferase NlaX (M·NlaX) from Neisseria lactamica was established to be
E.A. Kubareva +5 more
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Mammalian DNA (Cytosine-5-)-methyltransferase Expressed in Escherichia coli, Purified and Characterized [PDF]
Journal of Biological Chemistry, 1995 Besides modulating specific DNA-protein interactions, methylated cytosine, frequently referred to as the fifth base of the genome, also influences DNA structure, recombination, transposition, repair, transcription, imprinting, and mutagenesis. DNA (cytosine-5-)-methyltransferase catalyzes cytosine methylation in eukaryotes. We have cloned and expressed
T O, Tollefsbol, C A, Hutchison
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Recurrent acral ulcerations associated with DNA methyltransferase 1-complex disorder
Revista da Sociedade Portuguesa de Dermatologia e Venereologia, 2022 DNA methyltransferase 1, encoded by DNMT gene, is a crucial DNA methyltransferase with essential roles in transcription regulation, gene imprinting, and cell differentiation.
Diogo Cerejeira +4 more
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Library Construction for Genome-wide Bisulfite Sequencing in Plants
Bio-Protocol, 2013 DNA methylation is the most studied epigenetic modification, which involves the addition of a methyl group to the carbon-5 position of cytosine residues in DNA. DNA methylation is important for the regulation of gene expression.
David Amador +3 more
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A Modified Protocol for Bisulfite Genomic Sequencing of Difficult Samples
Biological Procedures Online, 2009 The bisulfite genomic sequencing protocol is a widely used method for analyzing DNA methylation. It relies on the deamination of unmethylated cytosine residues to uracil; however, its high rates of DNA degradation and incomplete cytosine to uracil ...
Toulouse André +2 more
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Communications Biology5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) are modified versions of cytosine in DNA with roles in regulating gene expression. Using whole genomic DNA from mouse cerebellum, we benchmark 5mC and 5hmC detection by Oxford Nanopore ...
Dominic Oliver Halliwell +4 more
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Plant cytosine-5 DNA methyltransferases: Structure, function, and molecular evolution
Genomics, 2007 A detailed analysis of the structure and function, along with evolutionary aspects, of the main plant cytosine-5 DNA methyltransferases (C5-MTases) is presented. The evolutionary relationships between the already known and four candidate plant C5-MTases identified in this work were investigated using the distance, maximum-parsimony, and maximum ...
Kossida, Sophia, Pavlopoulou, ATHANASIA
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