Results 71 to 80 of about 1,473,978 (184)

Inhibition of RNA polymerase II transcription in human cells by synthetic DNA-binding ligands [PDF]

, 1998
Sequence-specific DNA-binding small molecules that can permeate human cells potentially could regulate transcription of specific genes. Multiple cellular DNA-binding transcription factors are required by HIV type 1 for RNA synthesis.
Baird, Eldon E., Dervan, Peter B., Dickinson, Liliane A., Gottesfeld, Joel M., Gulizia, Richard J., Mosier, Donald E., Trauger, John W.   +6 more
core   +1 more source

Replicative bypass studies of l-deoxyribonucleosides in Vitro and in E. coli cell

Scientific Reports, 2022
l-nucleosides were the most important antiviral lead compounds because they can inhibit viral DNA polymerase and DNA synthesis of many viruses, whereas they may lead to mutations in DNA replication and cause genomic instability.
Yuhe Kan, Zhaoyang Jin, Yongqi Ke, Dao Lin, Liang Yan, Li Wu, Yujian He   +6 more
doaj   +1 more source

DNA Polymerase Gamma in Mitochondrial DNA Replication and Repair

The Scientific World Journal, 2003
Mutations in mitochondrial DNA (mtDNA) are associated with aging, and they can cause tissue degeneration and neuromuscular pathologies known as mitochondrial diseases.
William C. Copeland, Matthew J. Longley
doaj   +1 more source

Fusion of Taq DNA polymerase with single-stranded DNA binding-like protein of Nanoarchaeum equitans-Expression and characterization.

PLoS ONE, 2017
DNA polymerases are present in all organisms and are important enzymes that synthesise DNA molecules. They are used in various fields of science, predominantly as essential components for in vitro DNA syntheses, known as PCR.
Marcin Olszewski, Marta Śpibida, Maciej Bilek, Beata Krawczyk   +3 more
doaj   +1 more source

Capturing the ‘ome’ : the expanding molecular toolbox for RNA and DNA library construction [PDF]

, 2018
All sequencing experiments and most functional genomics screens rely on the generation of libraries to comprehensively capture pools of targeted sequences.
Boone, Morgane, Callewaert, Nico, De Koker, Andries   +2 more
core   +2 more sources

The trans-activation domain of the sporulation response regulator Spo0A revealed by X-ray crystallography [PDF]

, 2000
Sporulation in Bacillus involves the induction of scores of genes in a temporally and spatially co-ordinated programme of cell development. Its initiation is under the control of an expanded two-component signal transduction system termed a phosphorelay.
Baldus J.M., Brown D.P., Buckner C.M., Buckner C.M., Busby S., Chung J.D., Cowtan K., Ferrari F.A., Fogh R.H., Green B.D., Grimsley J.K., Hatt J.K., Igarashi K., Ireton K., Kenney T.J., Kondo H., Kuldell N., Makino K., Martinez-Hackert E., Muchova K., Murshudov G.N., Olmedo G., Perego M., Satola S.W., Schyns G., Spiegelman G.B., Spiegelman G.B., Strauch M.A., Strauch M.A., Terwilliger T.C., Weir J., Wu H.-M., York K.   +32 more
core   +1 more source

Cloning of Thermostable DNA Polymerase Gene from a Thermophilic Brevibacillus sp. Isolated from Sikidang Crater, Dieng Plateu, Central Java

Indonesian Journal of Biotechnology, 2010
Thermostable DNA polymerase has an important role for amplifying small amount of DNA through polymerase chain reaction (PCR). Thermophillic bacteria Brevibacillus sp. was isolated from Sikidang Crater, Dieng Plateu, Central Java.
Lucia Dhiantika Witasari, Irfan Dwidya Prijambada, Jaka Widada, Dionysius Andang Arif Wibawa   +3 more
doaj   +1 more source

New in vitro dna polymerase activity and fidelity assay reveals age-dependent changes in Arabidopsis Thaliana

International Journal of Plant Biology, 2011
DNA polymerase is an enzyme that adds nucleotides to the growing DNA chain during replication and DNA repair. DNA polymerase activity and fidelity are important characteristics that reflect the ability of DNA polymerase to add nucleotides and then ...
Andrey Golubov, Priti Maheshwari, Andriy Bilichak, Igor Kovalchuk   +3 more
doaj   +1 more source

Label-free optical detection of single enzyme-reactant reactions and associated conformational changes

, 2017
Monitoring the kinetics and conformational dynamics of single enzymes is crucial in order to better understand their biological functions as these motions and structural dynamics are usually unsynchronized among the molecules.
Baaske, Martin D., Kim, Eugene, Schuldes, Isabel, Vollmer, Frank, Wilsch, Peter S.   +4 more
core   +1 more source

Evaluation of Different Amplification Protocols for Use in Primer-Extension Preamplification

BioTechniques, 1996
Different amplification protocols were evaluated for use with primer-extension preamplification (PEP). We hypothesized that a protocol known to improve amplification of long DNA fragments would improve efficacy of PEP. Eight DNA samples were preamplified
Eduardo Casas, Brian W. Kirkpatrick
doaj   +1 more source