Results 51 to 60 of about 1,506,691 (357)
Bst polymerase — a humble relative of Taq polymerase
Computational and Structural Biotechnology Journal, 2023 DNA polymerases are a superfamily of enzymes synthesizing DNA using DNA as a template. They are essential for nucleic acid metabolism and for DNA replication and repair.
Igor Oscorbin, Maxim Filipenko
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An archaeal family-B DNA polymerase variant able to replicate past DNA damage: occurrence of replicative and translesion synthesis polymerases within the B family [PDF]
, 2014 A mutant of the high fidelity family-B DNA polymerase from the archaeon Thermococcus gorgonarius (Tgo-Pol), able to replicate past DNA lesions, is described.
Aidan J. Doherty +68 more
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Brazilian Journal of Medical and Biological Research, 1998 For certain applications of the polymerase chain reaction (PCR), it may be necessary to consider the accuracy of replication. The breakthrough that made PCR user friendly was the commercialization of Thermus aquaticus (Taq) DNA polymerase, an enzyme that
R.S. Diaz, E.C. Sabino
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PrimPol is required for replicative tolerance of G quadruplexes in vertebrate cells [PDF]
, 2016 G quadruplexes (G4s) can present potent blocks to DNA replication. Accurate and timely replication of G4s in vertebrates requires multiple specialized DNA helicases and polymerases to prevent genetic and epigenetic instability.
Bailey, Laura J +7 more
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Bacteriophage T7 DNA polymerase — Sequenase
Frontiers in Microbiology, 2014 An ideal DNA polymerase for chain-terminating DNA sequencing should possess the following features: 1) incorporate dideoxy- and other modified nucleotides at an efficiency similar to that of the cognate deoxynucleotides; 2) high processivity; 3) high ...
Bin eZhu
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A simple method for in-house Pfu DNA polymerase purification for high-fidelity PCR amplification
Iranian Journal of Microbiology, 2019 Background and Objectives: Pfu DNA polymerase is an enzyme that exhibits the lowest error rate in the 3´ to 5´ exonuclease (proofreading) activity during DNA synthesis in Polymerase Chain Reactions (PCRs).
Prabu Siva Sankar +6 more
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Direct Enzyme Engineering of B Family DNA Polymerases for Biotechnological Approaches
Bioengineering, 2023 DNA-dependent DNA polymerases have been intensively studied for more than 60 years and underlie numerous biotechnological and diagnostic applications.
Aleksandra A. Kuznetsova +1 more
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An analysis of the localisation and interactions of human p66 DNA polymerase δ subunit [PDF]
, 2005 DNA polymerase d is essential for eukaryotic DNA replication and also plays a role in DNA repair. The processivity of this polymerase complex is dependent upon its interaction with the sliding clamp PCNA and the polymerase-PCNA interaction is largely ...
J Richard G Pohler +2 more
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Scientific Reports, 2017 Organisms possess multiple DNA polymerases (Pols) and use each for a different purpose. One of the five Pols in Escherichia coli, DNA polymerase IV (Pol IV), encoded by the dinB gene, is known to participate in lesion bypass at certain DNA adducts.
Thanh Thi Le +3 more
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Fidelity of DNA polymerases in the detection of intraindividual variation of mitochondrial DNA
Mitochondrial DNA. Part B. Resources, 2020 Here we investigated the consequences of PCR amplification errors in the identification of intraindividual mtDNA variation. The bumblebee Bombus morio was chosen as model for the COI gene amplification tests with two DNA polymerases (Taq and Q5 ...
Paulo Cseri Ricardo +2 more
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