A simple method for in-house Pfu DNA polymerase purification for high-fidelity PCR amplification
Iranian Journal of Microbiology, 2019 Background and Objectives: Pfu DNA polymerase is an enzyme that exhibits the lowest error rate in the 3´ to 5´ exonuclease (proofreading) activity during DNA synthesis in Polymerase Chain Reactions (PCRs).
Prabu Siva Sankar +6 more
doaj +1 more source
Direct Enzyme Engineering of B Family DNA Polymerases for Biotechnological Approaches
Bioengineering, 2023 DNA-dependent DNA polymerases have been intensively studied for more than 60 years and underlie numerous biotechnological and diagnostic applications.
Aleksandra A. Kuznetsova +1 more
doaj +1 more source
Simultaneous Disruption of Two DNA Polymerases, Pol eta and Pol zeta, in Avian DT40 Cells Unmasks the Role of Pol eta in Cellular Response to Various DNA Lesions [PDF]
, 2010 Replicative DNA polymerases are frequently stalled by DNA lesions. The resulting replication blockage is released by homologous recombination (HR) and translesion DNA synthesis (TLS). TLS employs specialized TLS polymerases to bypass DNA lesions.
Sonoda, E +44 more
core +1 more source
Proofreading deficiency of Pol I increases the levels of spontaneous rpoB mutations in E. coli [PDF]
, 2011 The fidelity role of DNA polymerase I in chromosomal DNA replication in E. coli was investigated using the rpoB forward target. These experiments indicated that in a strain carrying a proofreading-exonuclease-defective form of Pol I (polAexo mutant) the ...
Jonczyk, Piotr +7 more
core +2 more sources
Scientific Reports, 2017 Organisms possess multiple DNA polymerases (Pols) and use each for a different purpose. One of the five Pols in Escherichia coli, DNA polymerase IV (Pol IV), encoded by the dinB gene, is known to participate in lesion bypass at certain DNA adducts.
Thanh Thi Le +3 more
doaj +1 more source
Bacteriophage T7 DNA polymerase — Sequenase
Frontiers in Microbiology, 2014 An ideal DNA polymerase for chain-terminating DNA sequencing should possess the following features: 1) incorporate dideoxy- and other modified nucleotides at an efficiency similar to that of the cognate deoxynucleotides; 2) high processivity; 3) high ...
Bin eZhu
doaj +1 more source
Fidelity of DNA polymerases in the detection of intraindividual variation of mitochondrial DNA
Mitochondrial DNA. Part B. Resources, 2020 Here we investigated the consequences of PCR amplification errors in the identification of intraindividual mtDNA variation. The bumblebee Bombus morio was chosen as model for the COI gene amplification tests with two DNA polymerases (Taq and Q5 ...
Paulo Cseri Ricardo +2 more
doaj +1 more source
Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation
FEBS Letters, EarlyView.Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz +11 more
wiley +1 more source
Functional mapping of the fission yeast DNA polymerase delta B-subunit Cdc1 by site-directed and random pentapeptide insertion mutagenesis [PDF]
, 2009 JSG was supported by a BBSRC Research Committee studentship, EVK by the Darwin Trust of Edinburgh, and FCG and SAM by a Wellcome Trust Senior Research Fellowship in Basic Biomedical Sciences.
Garcia, JS +5 more
core +1 more source
An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane
FEBS Letters, EarlyView.Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa +3 more
wiley +1 more source