Mismatch repair in recombination of bacteriophage T4
Biomolecular Concepts, 2012 The review focuses on the mechanism of mismatch repair in bacteriophage T4. It was first observed in T4 as an extra recombination mechanism, which contributed to the general recombination only when particular rII mutations were used as genetic markers ...
Shcherbakov Victor P.
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A novel role of DNA polymerase λ in translesion synthesis in conjunction with DNA polymerase ζ
Life Science Alliance, 2021 As an integral scaffolding component of DNA polymerase (Pol) zeta, Pol lambda adapts Pol zeta–dependent translesion synthesis to operate in a predominantly error-free manner in human cells.
Jung-Hoon Yoon +5 more
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Promutagenicity of 8-Chloroguanine, A Major Inflammation-Induced Halogenated DNA Lesion
Molecules, 2019 Chronic inflammation is closely associated with cancer development. One possible mechanism for inflammation-induced carcinogenesis is DNA damage caused by reactive halogen species, such as hypochlorous acid, which is released by myeloperoxidase to kill ...
Yi Kou, Myong-Chul Koag, Seongmin Lee
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A nuclear family A DNA polymerase from Entamoeba histolytica bypasses thymine glycol. [PDF]
PLoS Neglected Tropical Diseases, 2010 BACKGROUND: Eukaryotic family A DNA polymerases are involved in mitochondrial DNA replication or translesion DNA synthesis. Here, we present evidence that the sole family A DNA polymerase from the parasite protozoan E.
Guillermo Pastor-Palacios +2 more
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DNA Polymerase Profiling [PDF]
, 2008 We report a simple homogeneous fluorescence assay for quantification of DNA polymerase function in high throughput. The fluorescence signal is generated by the DNA polymerase triggering opening of a molecular beacon extension of the template strand. A resulting distance alteration is reported by fluorescence resonance energy transfer between two dyes ...
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Engineering processive DNA polymerases with maximum benefit and minimum cost
Frontiers in Microbiology, 2014 DNA polymerases need to be engineered to achieve optimal performance for biotechnological applications, which often require high fidelity replication when using modified nucleotides and when replicating difficult DNA sequences.
Linda J. Reha-Krantz +2 more
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Preparation of Phi29 DNA polymerase free of amplifiable DNA using ethidium monoazide, an ultraviolet-free light-emitting diode lamp and trehalose. [PDF]
PLoS ONE, 2014 We previously reported that multiply-primed rolling circle amplification (MRPCA) using modified random RNA primers can amplify tiny amounts of circular DNA without producing any byproducts.
Hirokazu Takahashi +10 more
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Cyclocytidine hydrochloride inhibits the synthesis of relaxed circular DNA of hepatitis B virus [PDF]
PeerJ, 2022 Background Cyclocytidine hydrochloride (HCl) has been reported to inhibit DNA synthesis by affecting DNA polymerase. Here, we tested the antiviral effect of cyclocytidine on hepatitis B virus (HBV) DNA synthesis, which is reliant on DNA polymerase ...
Xue Wang +7 more
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Live culture-based qPCR screening of Taq DNA polymerase variants for resistance to PCR inhibitors
Frontiers in Bioengineering and BiotechnologyWe present a live culture PCR (LC-PCR) workflow that enables direct screening of randomly mutagenized Thermus aquaticus (Taq) and Klentaq1 DNA polymerase libraries without enzyme purification.
Milko B. Kermekchiev +3 more
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Bst polymerase — a humble relative of Taq polymerase
Computational and Structural Biotechnology Journal, 2023 DNA polymerases are a superfamily of enzymes synthesizing DNA using DNA as a template. They are essential for nucleic acid metabolism and for DNA replication and repair.
Igor Oscorbin, Maxim Filipenko
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