A nuclear family A DNA polymerase from Entamoeba histolytica bypasses thymine glycol. [PDF]
PLoS Neglected Tropical Diseases, 2010 BACKGROUND: Eukaryotic family A DNA polymerases are involved in mitochondrial DNA replication or translesion DNA synthesis. Here, we present evidence that the sole family A DNA polymerase from the parasite protozoan E.
Guillermo Pastor-Palacios +2 more
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Functional mapping of the fission yeast DNA polymerase delta B-subunit Cdc1 by site-directed and random pentapeptide insertion mutagenesis [PDF]
, 2009 JSG was supported by a BBSRC Research Committee studentship, EVK by the Darwin Trust of Edinburgh, and FCG and SAM by a Wellcome Trust Senior Research Fellowship in Basic Biomedical Sciences.
Garcia, JS +5 more
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Engineering processive DNA polymerases with maximum benefit and minimum cost
Frontiers in Microbiology, 2014 DNA polymerases need to be engineered to achieve optimal performance for biotechnological applications, which often require high fidelity replication when using modified nucleotides and when replicating difficult DNA sequences.
Linda J. Reha-Krantz +2 more
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Preparation of Phi29 DNA polymerase free of amplifiable DNA using ethidium monoazide, an ultraviolet-free light-emitting diode lamp and trehalose. [PDF]
PLoS ONE, 2014 We previously reported that multiply-primed rolling circle amplification (MRPCA) using modified random RNA primers can amplify tiny amounts of circular DNA without producing any byproducts.
Hirokazu Takahashi +10 more
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Cyclocytidine hydrochloride inhibits the synthesis of relaxed circular DNA of hepatitis B virus [PDF]
PeerJ, 2022 Background Cyclocytidine hydrochloride (HCl) has been reported to inhibit DNA synthesis by affecting DNA polymerase. Here, we tested the antiviral effect of cyclocytidine on hepatitis B virus (HBV) DNA synthesis, which is reliant on DNA polymerase ...
Xue Wang +7 more
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Dynamics of DNA replication loops reveal temporal control of lagging-strand synthesis [PDF]
, 2009 In all organisms, the protein machinery responsible for the replication of DNA, the replisome, is faced with a directionality problem. The antiparallel nature of duplex DNA permits the leading-strand polymerase to advance in a continuous fashion, but ...
Richardson, Charles C., +15 more
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The UmuC subunit of the E. coli DNA polymerase V shows a unique interaction with the β-clamp processivity factor [PDF]
, 2013 Background Strict regulation of replisome components is essential to ensure the accurate transmission of the genome to the next generation. The sliding clamp processivity factors play a central role in this regulation, interacting with both DNA ...
Patoli, Atif A. +8 more
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Live culture-based qPCR screening of Taq DNA polymerase variants for resistance to PCR inhibitors
Frontiers in Bioengineering and BiotechnologyWe present a live culture PCR (LC-PCR) workflow that enables direct screening of randomly mutagenized Thermus aquaticus (Taq) and Klentaq1 DNA polymerase libraries without enzyme purification.
Milko B. Kermekchiev +3 more
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, 2006 In order to further understand how DNA polymerases discriminate against incorrect dNTPs, we synthesized two sets of dNTP analogues and tested them as substrates for DNA polymerase a (pol alpha) and Klenow fragment (exo-) of DNA polymerase I (Escherichia ...
Beckman, Jeff +5 more
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Brazilian Journal of Medical and Biological Research, 1998 For certain applications of the polymerase chain reaction (PCR), it may be necessary to consider the accuracy of replication. The breakthrough that made PCR user friendly was the commercialization of Thermus aquaticus (Taq) DNA polymerase, an enzyme that
R.S. Diaz, E.C. Sabino
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