Changes in the nuclear distribution of DNA polymerase alpha and PCNA/cyclin during the progress of the cell cycle, in a cell-free extract of Xenopus eggs [PDF]
, 1989 The nuclear distribution of DNA polymerase alpha and PCNA/cyclin in embryonic nuclei has been investigated, in a cell-free extract of Xenopus eggs that recapitulates a basic cell-cycle in vitro, by indirect immunofluorescence microscopy.
Kill, IR, Hutchison, C
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DNA polymerases in Biotechnology [PDF]
Frontiers in Microbiology, 2014 DNA polymerases are core tools for molecular biology including PCR, whole genome amplification, DNA sequencing and genotyping. Research has focused on discovery of novel DNA polymerases, characterization of DNA polymerase biochemistry and development of new replication assays. These studies have accelerated DNA polymerase engineering for biotechnology.
Gardner, Andrew F., Kelman, Zvi
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International Journal of Molecular Sciences, 2019 Maintenance of genome integrity is a key process in all organisms. DNA polymerases (Pols) are central players in this process as they are in charge of the faithful reproduction of the genetic information, as well as of DNA repair. Interestingly, all eukaryotes possess a large repertoire of polymerases.
Pedroza-Garcia, Jose-Antonio +2 more
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Accessibility of DNA in Chromatin to DNA Polymerase and RNA Polymerase [PDF]
Proceedings of the National Academy of Sciences, 1973 The accessibility of DNA in chromatin to both exogenous DNA polymerase and RNA polymerase is slight when compared to isolated DNA. DNA in extracted chromatin is somewhat more accessible to these enzymes than is DNA in the chromatin of isolated nuclei; and the DNA template of chromatin is more accessible to DNA polymerase than to RNA polymerase.
B, Silverman, A E, Mirsky
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dnaX36 mutator of Escherichia coli: effects of the tau subunit of the DNA polymerase III holoenzyme on chromosomal DNA replication fidelity. [PDF]
, 2011 The Escherichia coli dnaX36 mutant displays a mutator effect, reflecting a fidelity function of the dnaX-encoded τ subunit of the DNA polymerase III (Pol III) holoenzyme.
Jonczyk, Piotr +7 more
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Polymerase Mu Is a DNA-Directed DNA/RNA Polymerase [PDF]
Molecular and Cellular Biology, 2003 DNA polymerases are defined as such because they use deoxynucleotides instead of ribonucleotides with high specificity. We show here that polymerase mu (pol μ), implicated in the nonhomologous end-joining pathway for repair of DNA double-strand breaks, incorporates both ribonucleotides and deoxynucleotides in a template-directed manner.
Stephanie A, Nick McElhinny +1 more
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Discovery of cyanophage genomes which contain mitochondrial DNA polymerase [PDF]
, 2011 DNA polymerase γ is a family A DNA polymerase responsible for the replication of mitochondrial DNA in eukaryotes. The origins of DNA polymerase γ have remained elusive because it is not present in any known bacterium, though it has been hypothesized that
Whitworth, Anna L. +19 more
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Exploration of factors driving incorporation of unnatural dNTPS into DNA by Klenow fragment (DNA polymerase I) and DNA polymerase {alpha} [PDF]
, 2006 In order to further understand how DNA polymerases discriminate against incorrect dNTPs, we synthesized two sets of dNTP analogues and tested them as substrates for DNA polymerase a (pol alpha) and Klenow fragment (exo-) of DNA polymerase I (Escherichia ...
Beckman, Jeff +5 more
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DNA Helicase–Polymerase Coupling in Bacteriophage DNA Replication
Viruses, 2021 Bacteriophages have long been model systems to study the molecular mechanisms of DNA replication. During DNA replication, a DNA helicase and a DNA polymerase cooperatively unwind the parental DNA.
Chen-Yu Lo, Yang Gao
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REV1 restrains DNA polymerase zeta to ensure frame fidelity during translesion synthesis of UV photoproducts in vivo [PDF]
, 2008 Exposure to ultraviolet light induces a number of forms of damage in DNA, of which (6-4) photoproducts present the most formidable challenge to DNA replication.
Marcus, AP +4 more
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